Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5115 and Pf1N1B4_5116 are separated by 29 nucleotides Pf1N1B4_5116 and Pf1N1B4_5117 are separated by 84 nucleotides
Pf1N1B4_5115 - sucrose ABC transporter, ATPase component <small>(from data)</small>, at 5,674,741 to 5,675,886
_5115
Pf1N1B4_5116 - Sucrose alpha-glucosidase (EC 3.2.1.48) <small>(from data)</small>, at 5,675,916 to 5,677,385
_5116
Pf1N1B4_5117 - Sucrose specific transcriptional regulator CscR, LacI family, at 5,677,470 to 5,678,453
_5117
Position (kb) 5675
5676
5677
5678 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 5674.999 kb on + strand, within Pf1N1B4_5115 at 5675.305 kb on + strand, within Pf1N1B4_5115 at 5675.306 kb on - strand, within Pf1N1B4_5115 at 5675.404 kb on + strand, within Pf1N1B4_5115 at 5675.404 kb on + strand at 5675.405 kb on - strand, within Pf1N1B4_5115 at 5675.654 kb on - strand, within Pf1N1B4_5115 at 5675.671 kb on + strand, within Pf1N1B4_5115 at 5675.671 kb on + strand, within Pf1N1B4_5115 at 5675.671 kb on + strand at 5675.672 kb on - strand, within Pf1N1B4_5115 at 5675.883 kb on - strand at 5676.046 kb on - strand at 5676.073 kb on - strand, within Pf1N1B4_5116 at 5676.073 kb on - strand at 5676.111 kb on + strand, within Pf1N1B4_5116 at 5676.249 kb on + strand, within Pf1N1B4_5116 at 5676.250 kb on - strand, within Pf1N1B4_5116 at 5676.409 kb on + strand, within Pf1N1B4_5116 at 5676.433 kb on - strand, within Pf1N1B4_5116 at 5676.501 kb on - strand, within Pf1N1B4_5116 at 5676.501 kb on - strand, within Pf1N1B4_5116 at 5676.519 kb on + strand, within Pf1N1B4_5116 at 5676.549 kb on + strand at 5676.550 kb on - strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.551 kb on + strand, within Pf1N1B4_5116 at 5676.552 kb on - strand, within Pf1N1B4_5116 at 5676.595 kb on + strand, within Pf1N1B4_5116 at 5676.596 kb on - strand, within Pf1N1B4_5116 at 5676.596 kb on - strand at 5676.596 kb on - strand, within Pf1N1B4_5116 at 5676.596 kb on - strand, within Pf1N1B4_5116 at 5676.603 kb on + strand, within Pf1N1B4_5116 at 5676.604 kb on - strand, within Pf1N1B4_5116 at 5676.652 kb on - strand at 5677.104 kb on + strand, within Pf1N1B4_5116 at 5677.104 kb on + strand, within Pf1N1B4_5116 at 5677.529 kb on + strand at 5677.744 kb on + strand, within Pf1N1B4_5117 at 5677.930 kb on - strand at 5678.352 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 5,674,999 + Pf1N1B4_5115 0.23 -3.1 5,675,305 + Pf1N1B4_5115 0.49 -1.7 5,675,306 - Pf1N1B4_5115 0.49 -2.4 5,675,404 + Pf1N1B4_5115 0.58 -2.5 5,675,404 + -1.5 5,675,405 - Pf1N1B4_5115 0.58 -3.6 5,675,654 - Pf1N1B4_5115 0.80 -4.4 5,675,671 + Pf1N1B4_5115 0.81 -2.1 5,675,671 + Pf1N1B4_5115 0.81 -1.2 5,675,671 + -2.3 5,675,672 - Pf1N1B4_5115 0.81 -1.6 5,675,883 - -4.0 5,676,046 - -1.5 5,676,073 - Pf1N1B4_5116 0.11 -2.2 5,676,073 - -1.0 5,676,111 + Pf1N1B4_5116 0.13 -1.9 5,676,249 + Pf1N1B4_5116 0.23 -2.7 5,676,250 - Pf1N1B4_5116 0.23 +0.5 5,676,409 + Pf1N1B4_5116 0.34 -3.5 5,676,433 - Pf1N1B4_5116 0.35 -0.2 5,676,501 - Pf1N1B4_5116 0.40 +0.4 5,676,501 - Pf1N1B4_5116 0.40 -2.2 5,676,519 + Pf1N1B4_5116 0.41 -6.1 5,676,549 + -1.3 5,676,550 - Pf1N1B4_5116 0.43 -3.1 5,676,551 + Pf1N1B4_5116 0.43 -3.1 5,676,551 + Pf1N1B4_5116 0.43 -0.7 5,676,551 + Pf1N1B4_5116 0.43 -2.0 5,676,551 + Pf1N1B4_5116 0.43 -1.5 5,676,551 + Pf1N1B4_5116 0.43 -2.6 5,676,551 + Pf1N1B4_5116 0.43 -3.8 5,676,551 + Pf1N1B4_5116 0.43 -2.2 5,676,552 - Pf1N1B4_5116 0.43 -3.5 5,676,595 + Pf1N1B4_5116 0.46 -2.8 5,676,596 - Pf1N1B4_5116 0.46 -1.6 5,676,596 - +0.8 5,676,596 - Pf1N1B4_5116 0.46 -2.2 5,676,596 - Pf1N1B4_5116 0.46 -6.2 5,676,603 + Pf1N1B4_5116 0.47 -2.7 5,676,604 - Pf1N1B4_5116 0.47 -3.3 5,676,652 - -1.0 5,677,104 + Pf1N1B4_5116 0.81 -4.6 5,677,104 + Pf1N1B4_5116 0.81 -1.4 5,677,529 + +0.4 5,677,744 + Pf1N1B4_5117 0.28 +1.7 5,677,930 - +1.2 5,678,352 + -1.0
Or see this region's nucleotide sequence