Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4512 and Pf1N1B4_4513 are separated by 45 nucleotides Pf1N1B4_4513 and Pf1N1B4_4514 are separated by 252 nucleotides Pf1N1B4_4514 and Pf1N1B4_4515 are separated by 112 nucleotides
Pf1N1B4_4512 - PhaK-like protein, at 4,974,467 to 4,975,654
_4512
Pf1N1B4_4513 - hypothetical protein, at 4,975,700 to 4,975,846
_4513
Pf1N1B4_4514 - Transcriptional regulator RutR of pyrimidine catabolism (TetR family), at 4,976,099 to 4,976,719
_4514
Pf1N1B4_4515 - Dihydropyrimidine dehydrogenase [NADP+] (EC 1.3.1.2), at 4,976,832 to 4,978,106
_4515
Position (kb)
4976
4977 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4975.455 kb on + strand, within Pf1N1B4_4512 at 4975.456 kb on - strand, within Pf1N1B4_4512 at 4975.460 kb on - strand, within Pf1N1B4_4512 at 4975.588 kb on + strand at 4976.020 kb on + strand at 4976.246 kb on + strand, within Pf1N1B4_4514 at 4976.249 kb on + strand, within Pf1N1B4_4514 at 4976.249 kb on + strand, within Pf1N1B4_4514 at 4976.250 kb on - strand, within Pf1N1B4_4514 at 4976.250 kb on - strand, within Pf1N1B4_4514 at 4976.274 kb on - strand, within Pf1N1B4_4514 at 4976.274 kb on - strand, within Pf1N1B4_4514 at 4976.565 kb on + strand at 4976.567 kb on + strand, within Pf1N1B4_4514 at 4976.567 kb on + strand, within Pf1N1B4_4514 at 4976.567 kb on + strand, within Pf1N1B4_4514 at 4976.567 kb on + strand, within Pf1N1B4_4514 at 4976.567 kb on + strand, within Pf1N1B4_4514 at 4976.567 kb on + strand, within Pf1N1B4_4514 at 4976.568 kb on - strand at 4976.568 kb on - strand, within Pf1N1B4_4514 at 4976.568 kb on - strand, within Pf1N1B4_4514 at 4976.620 kb on + strand, within Pf1N1B4_4514 at 4976.621 kb on - strand, within Pf1N1B4_4514 at 4976.984 kb on - strand at 4976.984 kb on - strand, within Pf1N1B4_4515 at 4976.984 kb on - strand, within Pf1N1B4_4515 at 4976.988 kb on - strand, within Pf1N1B4_4515 at 4976.988 kb on - strand, within Pf1N1B4_4515 at 4977.036 kb on - strand, within Pf1N1B4_4515 at 4977.106 kb on + strand, within Pf1N1B4_4515 at 4977.107 kb on - strand, within Pf1N1B4_4515 at 4977.122 kb on - strand at 4977.262 kb on - strand, within Pf1N1B4_4515 at 4977.607 kb on + strand at 4977.607 kb on + strand, within Pf1N1B4_4515 at 4977.608 kb on - strand at 4977.608 kb on - strand, within Pf1N1B4_4515 at 4977.641 kb on - strand, within Pf1N1B4_4515
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 4,975,455 + Pf1N1B4_4512 0.83 -3.2 4,975,456 - Pf1N1B4_4512 0.83 -0.9 4,975,460 - Pf1N1B4_4512 0.84 -2.9 4,975,588 + -1.8 4,976,020 + +0.8 4,976,246 + Pf1N1B4_4514 0.24 +0.8 4,976,249 + Pf1N1B4_4514 0.24 -1.9 4,976,249 + Pf1N1B4_4514 0.24 -1.4 4,976,250 - Pf1N1B4_4514 0.24 -1.9 4,976,250 - Pf1N1B4_4514 0.24 -0.4 4,976,274 - Pf1N1B4_4514 0.28 -0.1 4,976,274 - Pf1N1B4_4514 0.28 -0.1 4,976,565 + -1.1 4,976,567 + Pf1N1B4_4514 0.75 +1.4 4,976,567 + Pf1N1B4_4514 0.75 -0.5 4,976,567 + Pf1N1B4_4514 0.75 -0.7 4,976,567 + Pf1N1B4_4514 0.75 -0.7 4,976,567 + Pf1N1B4_4514 0.75 +1.3 4,976,567 + Pf1N1B4_4514 0.75 +0.1 4,976,568 - -1.4 4,976,568 - Pf1N1B4_4514 0.76 +0.9 4,976,568 - Pf1N1B4_4514 0.76 -1.2 4,976,620 + Pf1N1B4_4514 0.84 -0.7 4,976,621 - Pf1N1B4_4514 0.84 -0.6 4,976,984 - +0.6 4,976,984 - Pf1N1B4_4515 0.12 -1.3 4,976,984 - Pf1N1B4_4515 0.12 -0.4 4,976,988 - Pf1N1B4_4515 0.12 +1.0 4,976,988 - Pf1N1B4_4515 0.12 +2.5 4,977,036 - Pf1N1B4_4515 0.16 +0.0 4,977,106 + Pf1N1B4_4515 0.21 +0.8 4,977,107 - Pf1N1B4_4515 0.22 -1.3 4,977,122 - -1.4 4,977,262 - Pf1N1B4_4515 0.34 +0.4 4,977,607 + +2.1 4,977,607 + Pf1N1B4_4515 0.61 -0.1 4,977,608 - -1.8 4,977,608 - Pf1N1B4_4515 0.61 -1.6 4,977,641 - Pf1N1B4_4515 0.63 +1.0
Or see this region's nucleotide sequence