Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4458 and Pf1N1B4_4459 overlap by 11 nucleotides Pf1N1B4_4459 and Pf1N1B4_4460 are separated by 187 nucleotides Pf1N1B4_4460 and Pf1N1B4_4461 are separated by 142 nucleotides
Pf1N1B4_4458 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 4,918,049 to 4,919,530
_4458
Pf1N1B4_4459 - Thermostable hemolysin delta-VPH, at 4,919,520 to 4,920,197
_4459
Pf1N1B4_4460 - Cytochrome b561, at 4,920,385 to 4,920,957
_4460
Pf1N1B4_4461 - Glutathione-regulated potassium-efflux system protein KefB, at 4,921,100 to 4,922,908
_4461
Position (kb)
4919
4920
4921 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4918.662 kb on + strand, within Pf1N1B4_4458 at 4918.662 kb on + strand at 4918.663 kb on - strand, within Pf1N1B4_4458 at 4919.095 kb on + strand, within Pf1N1B4_4458 at 4919.430 kb on + strand at 4919.430 kb on + strand at 4919.431 kb on - strand at 4919.648 kb on - strand, within Pf1N1B4_4459 at 4919.797 kb on + strand, within Pf1N1B4_4459 at 4919.797 kb on + strand at 4919.797 kb on + strand at 4919.798 kb on - strand, within Pf1N1B4_4459 at 4920.235 kb on - strand at 4920.285 kb on - strand at 4920.384 kb on + strand at 4920.384 kb on + strand at 4920.385 kb on - strand at 4920.404 kb on + strand at 4920.404 kb on + strand at 4920.469 kb on + strand, within Pf1N1B4_4460 at 4920.673 kb on + strand, within Pf1N1B4_4460 at 4920.743 kb on - strand, within Pf1N1B4_4460 at 4921.049 kb on + strand at 4921.050 kb on - strand at 4921.050 kb on - strand at 4921.050 kb on - strand at 4921.050 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 4,918,662 + Pf1N1B4_4458 0.41 +0.5 4,918,662 + -1.7 4,918,663 - Pf1N1B4_4458 0.41 -1.1 4,919,095 + Pf1N1B4_4458 0.71 -3.1 4,919,430 + -2.7 4,919,430 + -1.7 4,919,431 - -2.3 4,919,648 - Pf1N1B4_4459 0.19 +1.3 4,919,797 + Pf1N1B4_4459 0.41 +1.1 4,919,797 + -0.9 4,919,797 + +0.3 4,919,798 - Pf1N1B4_4459 0.41 +0.8 4,920,235 - +1.7 4,920,285 - -1.8 4,920,384 + +0.6 4,920,384 + +1.8 4,920,385 - +1.9 4,920,404 + +0.6 4,920,404 + -2.5 4,920,469 + Pf1N1B4_4460 0.15 -2.3 4,920,673 + Pf1N1B4_4460 0.50 -1.8 4,920,743 - Pf1N1B4_4460 0.62 -0.1 4,921,049 + -4.0 4,921,050 - +2.1 4,921,050 - -2.6 4,921,050 - +0.9 4,921,050 - -0.7
Or see this region's nucleotide sequence