Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3364 and Pf1N1B4_3365 are separated by 147 nucleotides Pf1N1B4_3365 and Pf1N1B4_3366 are separated by 78 nucleotides Pf1N1B4_3366 and Pf1N1B4_3367 overlap by 4 nucleotides
Pf1N1B4_3364 - Cyn operon transcriptional activator, at 3,751,948 to 3,752,865
_3364
Pf1N1B4_3365 - Sialic acid transporter (permease) NanT, at 3,753,013 to 3,754,296
_3365
Pf1N1B4_3366 - Lactam utilization protein LamB, at 3,754,375 to 3,755,127
_3366
Pf1N1B4_3367 - Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54), at 3,755,124 to 3,755,828
_3367
Position (kb)
3753
3754
3755 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3752.182 kb on + strand, within Pf1N1B4_3364 at 3752.183 kb on - strand, within Pf1N1B4_3364 at 3752.295 kb on + strand, within Pf1N1B4_3364 at 3752.295 kb on + strand, within Pf1N1B4_3364 at 3752.334 kb on - strand, within Pf1N1B4_3364 at 3752.334 kb on - strand, within Pf1N1B4_3364 at 3752.345 kb on + strand, within Pf1N1B4_3364 at 3752.882 kb on + strand at 3752.882 kb on + strand at 3752.904 kb on + strand at 3752.904 kb on + strand at 3752.984 kb on + strand at 3752.984 kb on + strand at 3752.984 kb on + strand at 3753.074 kb on - strand at 3753.102 kb on + strand at 3753.102 kb on + strand at 3753.364 kb on + strand, within Pf1N1B4_3365 at 3753.364 kb on + strand, within Pf1N1B4_3365 at 3753.364 kb on + strand at 3753.365 kb on - strand, within Pf1N1B4_3365 at 3753.365 kb on - strand, within Pf1N1B4_3365 at 3753.669 kb on + strand, within Pf1N1B4_3365 at 3753.669 kb on + strand, within Pf1N1B4_3365 at 3753.778 kb on + strand, within Pf1N1B4_3365 at 3753.778 kb on + strand, within Pf1N1B4_3365 at 3753.833 kb on + strand, within Pf1N1B4_3365 at 3753.834 kb on - strand, within Pf1N1B4_3365 at 3753.877 kb on + strand, within Pf1N1B4_3365 at 3753.877 kb on + strand, within Pf1N1B4_3365 at 3753.877 kb on + strand, within Pf1N1B4_3365 at 3753.977 kb on - strand, within Pf1N1B4_3365 at 3754.123 kb on + strand, within Pf1N1B4_3365 at 3754.123 kb on + strand, within Pf1N1B4_3365 at 3754.123 kb on + strand, within Pf1N1B4_3365 at 3754.316 kb on + strand at 3754.330 kb on + strand at 3754.330 kb on + strand at 3754.330 kb on + strand at 3754.330 kb on + strand at 3754.330 kb on + strand at 3754.331 kb on - strand at 3754.331 kb on - strand at 3754.331 kb on - strand at 3754.360 kb on - strand at 3754.389 kb on - strand at 3754.571 kb on - strand, within Pf1N1B4_3366 at 3754.609 kb on + strand, within Pf1N1B4_3366 at 3754.609 kb on + strand, within Pf1N1B4_3366 at 3754.696 kb on + strand, within Pf1N1B4_3366 at 3754.696 kb on + strand, within Pf1N1B4_3366 at 3754.696 kb on + strand, within Pf1N1B4_3366 at 3754.697 kb on - strand, within Pf1N1B4_3366 at 3754.697 kb on - strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand, within Pf1N1B4_3366 at 3754.720 kb on + strand at 3754.721 kb on - strand, within Pf1N1B4_3366 at 3754.852 kb on + strand, within Pf1N1B4_3366 at 3754.853 kb on - strand, within Pf1N1B4_3366 at 3754.853 kb on - strand, within Pf1N1B4_3366 at 3754.894 kb on + strand, within Pf1N1B4_3366 at 3754.894 kb on + strand, within Pf1N1B4_3366 at 3754.895 kb on - strand, within Pf1N1B4_3366 at 3755.040 kb on + strand, within Pf1N1B4_3366 at 3755.040 kb on + strand, within Pf1N1B4_3366 at 3755.040 kb on + strand, within Pf1N1B4_3366 at 3755.041 kb on - strand, within Pf1N1B4_3366 at 3755.041 kb on - strand, within Pf1N1B4_3366 at 3755.051 kb on + strand, within Pf1N1B4_3366 at 3755.051 kb on + strand, within Pf1N1B4_3366 at 3755.051 kb on + strand at 3755.052 kb on - strand at 3755.137 kb on + strand at 3755.198 kb on + strand, within Pf1N1B4_3367 at 3755.198 kb on + strand, within Pf1N1B4_3367 at 3755.274 kb on + strand, within Pf1N1B4_3367 at 3755.295 kb on + strand, within Pf1N1B4_3367
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 3,752,182 + Pf1N1B4_3364 0.25 -1.0 3,752,183 - Pf1N1B4_3364 0.26 +0.8 3,752,295 + Pf1N1B4_3364 0.38 -0.5 3,752,295 + Pf1N1B4_3364 0.38 +1.9 3,752,334 - Pf1N1B4_3364 0.42 -1.1 3,752,334 - Pf1N1B4_3364 0.42 -1.8 3,752,345 + Pf1N1B4_3364 0.43 -1.2 3,752,882 + -0.6 3,752,882 + +0.3 3,752,904 + +1.0 3,752,904 + +2.5 3,752,984 + -1.4 3,752,984 + +1.3 3,752,984 + -0.5 3,753,074 - +1.5 3,753,102 + -1.6 3,753,102 + -1.3 3,753,364 + Pf1N1B4_3365 0.27 -2.1 3,753,364 + Pf1N1B4_3365 0.27 +1.0 3,753,364 + -1.6 3,753,365 - Pf1N1B4_3365 0.27 -1.1 3,753,365 - Pf1N1B4_3365 0.27 -1.2 3,753,669 + Pf1N1B4_3365 0.51 -2.7 3,753,669 + Pf1N1B4_3365 0.51 +0.4 3,753,778 + Pf1N1B4_3365 0.60 +1.2 3,753,778 + Pf1N1B4_3365 0.60 +0.9 3,753,833 + Pf1N1B4_3365 0.64 +2.6 3,753,834 - Pf1N1B4_3365 0.64 +2.5 3,753,877 + Pf1N1B4_3365 0.67 -1.6 3,753,877 + Pf1N1B4_3365 0.67 +0.9 3,753,877 + Pf1N1B4_3365 0.67 -2.1 3,753,977 - Pf1N1B4_3365 0.75 +2.2 3,754,123 + Pf1N1B4_3365 0.86 +1.1 3,754,123 + Pf1N1B4_3365 0.86 +1.2 3,754,123 + Pf1N1B4_3365 0.86 -0.5 3,754,316 + +0.6 3,754,330 + +1.8 3,754,330 + -0.8 3,754,330 + -0.4 3,754,330 + +1.7 3,754,330 + -0.7 3,754,331 - -2.4 3,754,331 - -1.8 3,754,331 - -2.3 3,754,360 - +0.3 3,754,389 - -1.9 3,754,571 - Pf1N1B4_3366 0.26 +1.5 3,754,609 + Pf1N1B4_3366 0.31 +1.8 3,754,609 + Pf1N1B4_3366 0.31 -2.0 3,754,696 + Pf1N1B4_3366 0.43 +2.1 3,754,696 + Pf1N1B4_3366 0.43 +0.7 3,754,696 + Pf1N1B4_3366 0.43 -0.6 3,754,697 - Pf1N1B4_3366 0.43 +1.7 3,754,697 - Pf1N1B4_3366 0.43 +1.1 3,754,720 + Pf1N1B4_3366 0.46 -0.0 3,754,720 + Pf1N1B4_3366 0.46 +2.2 3,754,720 + Pf1N1B4_3366 0.46 +0.2 3,754,720 + Pf1N1B4_3366 0.46 -0.6 3,754,720 + Pf1N1B4_3366 0.46 -2.5 3,754,720 + +0.7 3,754,721 - Pf1N1B4_3366 0.46 -1.0 3,754,852 + Pf1N1B4_3366 0.63 -0.5 3,754,853 - Pf1N1B4_3366 0.63 -2.1 3,754,853 - Pf1N1B4_3366 0.63 -0.4 3,754,894 + Pf1N1B4_3366 0.69 -0.0 3,754,894 + Pf1N1B4_3366 0.69 -0.1 3,754,895 - Pf1N1B4_3366 0.69 +0.6 3,755,040 + Pf1N1B4_3366 0.88 -0.4 3,755,040 + Pf1N1B4_3366 0.88 +1.2 3,755,040 + Pf1N1B4_3366 0.88 -2.3 3,755,041 - Pf1N1B4_3366 0.88 -2.6 3,755,041 - Pf1N1B4_3366 0.88 +0.5 3,755,051 + Pf1N1B4_3366 0.90 -1.4 3,755,051 + Pf1N1B4_3366 0.90 -1.3 3,755,051 + +1.7 3,755,052 - +1.2 3,755,137 + -1.6 3,755,198 + Pf1N1B4_3367 0.10 -1.4 3,755,198 + Pf1N1B4_3367 0.10 +0.5 3,755,274 + Pf1N1B4_3367 0.21 +0.5 3,755,295 + Pf1N1B4_3367 0.24 +1.7
Or see this region's nucleotide sequence