Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3354 and Pf1N1B4_3355 are separated by 96 nucleotides Pf1N1B4_3355 and Pf1N1B4_3356 overlap by 4 nucleotides Pf1N1B4_3356 and Pf1N1B4_3357 are separated by 165 nucleotides
Pf1N1B4_3354 - FIG00954793: hypothetical protein, at 3,740,471 to 3,741,157
_3354
Pf1N1B4_3355 - FIG00955239: hypothetical protein, at 3,741,254 to 3,741,544
_3355
Pf1N1B4_3356 - Cyclic nucleotide-binding protein, at 3,741,541 to 3,742,065
_3356
Pf1N1B4_3357 - Protease II (EC 3.4.21.83), at 3,742,231 to 3,744,285
_3357
Position (kb)
3741
3742
3743 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3740.671 kb on - strand, within Pf1N1B4_3354 at 3740.671 kb on - strand at 3740.783 kb on - strand, within Pf1N1B4_3354 at 3740.783 kb on - strand, within Pf1N1B4_3354 at 3741.088 kb on - strand, within Pf1N1B4_3354 at 3741.184 kb on + strand at 3741.184 kb on + strand at 3741.185 kb on - strand at 3741.185 kb on - strand at 3741.998 kb on - strand, within Pf1N1B4_3356 at 3741.998 kb on - strand, within Pf1N1B4_3356 at 3742.204 kb on + strand at 3742.204 kb on + strand at 3742.204 kb on + strand at 3742.204 kb on + strand at 3742.205 kb on - strand at 3742.276 kb on + strand at 3742.277 kb on - strand at 3742.286 kb on - strand at 3742.286 kb on - strand at 3742.286 kb on - strand at 3742.286 kb on - strand at 3742.407 kb on - strand at 3742.407 kb on - strand at 3742.488 kb on - strand, within Pf1N1B4_3357 at 3742.488 kb on - strand, within Pf1N1B4_3357 at 3742.505 kb on + strand, within Pf1N1B4_3357 at 3742.505 kb on + strand, within Pf1N1B4_3357 at 3742.505 kb on + strand, within Pf1N1B4_3357 at 3742.506 kb on - strand, within Pf1N1B4_3357 at 3742.506 kb on - strand, within Pf1N1B4_3357 at 3742.541 kb on + strand, within Pf1N1B4_3357 at 3742.542 kb on - strand, within Pf1N1B4_3357 at 3742.825 kb on + strand, within Pf1N1B4_3357 at 3742.825 kb on + strand, within Pf1N1B4_3357 at 3742.826 kb on - strand, within Pf1N1B4_3357 at 3742.928 kb on + strand, within Pf1N1B4_3357 at 3742.928 kb on + strand, within Pf1N1B4_3357 at 3743.027 kb on + strand, within Pf1N1B4_3357 at 3743.027 kb on + strand, within Pf1N1B4_3357 at 3743.027 kb on + strand, within Pf1N1B4_3357 at 3743.028 kb on - strand, within Pf1N1B4_3357 at 3743.028 kb on - strand, within Pf1N1B4_3357 at 3743.028 kb on - strand, within Pf1N1B4_3357
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 3,740,671 - Pf1N1B4_3354 0.29 +0.1 3,740,671 - -1.9 3,740,783 - Pf1N1B4_3354 0.45 +1.6 3,740,783 - Pf1N1B4_3354 0.45 +0.0 3,741,088 - Pf1N1B4_3354 0.90 +0.8 3,741,184 + +1.2 3,741,184 + +1.0 3,741,185 - -0.3 3,741,185 - -1.3 3,741,998 - Pf1N1B4_3356 0.87 -2.4 3,741,998 - Pf1N1B4_3356 0.87 -1.2 3,742,204 + +0.2 3,742,204 + -0.6 3,742,204 + +2.9 3,742,204 + +1.0 3,742,205 - -0.3 3,742,276 + +1.7 3,742,277 - +1.2 3,742,286 - -1.5 3,742,286 - +0.0 3,742,286 - +0.5 3,742,286 - +0.9 3,742,407 - +1.8 3,742,407 - +0.2 3,742,488 - Pf1N1B4_3357 0.13 -0.3 3,742,488 - Pf1N1B4_3357 0.13 -2.5 3,742,505 + Pf1N1B4_3357 0.13 -0.3 3,742,505 + Pf1N1B4_3357 0.13 -2.8 3,742,505 + Pf1N1B4_3357 0.13 -1.5 3,742,506 - Pf1N1B4_3357 0.13 -0.9 3,742,506 - Pf1N1B4_3357 0.13 -0.7 3,742,541 + Pf1N1B4_3357 0.15 -2.2 3,742,542 - Pf1N1B4_3357 0.15 +0.8 3,742,825 + Pf1N1B4_3357 0.29 +0.2 3,742,825 + Pf1N1B4_3357 0.29 -1.8 3,742,826 - Pf1N1B4_3357 0.29 -2.5 3,742,928 + Pf1N1B4_3357 0.34 +0.7 3,742,928 + Pf1N1B4_3357 0.34 +0.1 3,743,027 + Pf1N1B4_3357 0.39 -1.6 3,743,027 + Pf1N1B4_3357 0.39 +1.1 3,743,027 + Pf1N1B4_3357 0.39 -0.7 3,743,028 - Pf1N1B4_3357 0.39 -0.6 3,743,028 - Pf1N1B4_3357 0.39 -0.6 3,743,028 - Pf1N1B4_3357 0.39 -3.4
Or see this region's nucleotide sequence