Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2824 and Pf1N1B4_5202 are separated by 300 nucleotides Pf1N1B4_5202 and Pf1N1B4_5203 are separated by 12 nucleotides Pf1N1B4_5203 and Pf1N1B4_2825 are separated by 109 nucleotides Pf1N1B4_2825 and Pf1N1B4_2826 are separated by 340 nucleotides
Pf1N1B4_2824 - ClpB protein, at 3,167,042 to 3,169,606
_2824
Pf1N1B4_5202 - tRNA-Asn-GTT, at 3,169,907 to 3,169,979
_5202
Pf1N1B4_5203 - tRNA-Pro-TGG, at 3,169,992 to 3,170,065
_5203
Pf1N1B4_2825 - EF hand domain protein, at 3,170,175 to 3,170,576
_2825
Pf1N1B4_2826 - NADH dehydrogenase (EC 1.6.99.3), at 3,170,917 to 3,172,215
_2826
Position (kb)
3170
3171 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3169.175 kb on - strand, within Pf1N1B4_2824 at 3169.438 kb on + strand at 3169.457 kb on + strand at 3169.547 kb on + strand at 3169.547 kb on + strand at 3169.691 kb on + strand at 3169.753 kb on + strand at 3169.753 kb on + strand at 3170.069 kb on + strand at 3170.072 kb on + strand at 3170.072 kb on + strand at 3170.072 kb on + strand at 3170.176 kb on + strand at 3170.176 kb on + strand at 3170.176 kb on + strand at 3170.176 kb on + strand at 3170.177 kb on - strand at 3170.177 kb on - strand at 3170.177 kb on - strand at 3170.177 kb on - strand at 3170.180 kb on + strand at 3170.180 kb on + strand at 3170.180 kb on + strand at 3170.180 kb on + strand at 3170.181 kb on - strand at 3170.181 kb on - strand at 3170.181 kb on - strand at 3170.181 kb on - strand at 3170.181 kb on - strand at 3170.236 kb on + strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.237 kb on - strand, within Pf1N1B4_2825 at 3170.418 kb on - strand, within Pf1N1B4_2825 at 3170.418 kb on - strand, within Pf1N1B4_2825 at 3170.418 kb on - strand, within Pf1N1B4_2825 at 3170.461 kb on + strand, within Pf1N1B4_2825 at 3170.462 kb on - strand, within Pf1N1B4_2825 at 3170.462 kb on - strand, within Pf1N1B4_2825 at 3170.562 kb on - strand at 3170.577 kb on + strand at 3170.578 kb on - strand at 3170.578 kb on - strand at 3170.578 kb on - strand at 3170.699 kb on - strand at 3170.724 kb on + strand at 3170.724 kb on + strand at 3170.724 kb on + strand at 3170.724 kb on + strand at 3170.753 kb on + strand at 3170.753 kb on + strand at 3170.753 kb on + strand at 3170.754 kb on - strand at 3170.869 kb on + strand at 3170.899 kb on + strand at 3170.903 kb on + strand at 3170.904 kb on - strand at 3170.904 kb on - strand at 3170.919 kb on - strand at 3170.919 kb on - strand at 3170.996 kb on + strand at 3170.996 kb on + strand at 3170.997 kb on - strand at 3170.998 kb on + strand at 3170.998 kb on + strand at 3170.998 kb on + strand at 3170.998 kb on + strand at 3170.999 kb on - strand at 3170.999 kb on - strand at 3170.999 kb on - strand at 3170.999 kb on - strand at 3170.999 kb on - strand at 3171.020 kb on + strand at 3171.028 kb on + strand at 3171.029 kb on - strand at 3171.033 kb on - strand at 3171.033 kb on - strand at 3171.033 kb on - strand at 3171.134 kb on + strand, within Pf1N1B4_2826 at 3171.135 kb on - strand, within Pf1N1B4_2826 at 3171.135 kb on - strand, within Pf1N1B4_2826 at 3171.143 kb on + strand, within Pf1N1B4_2826 at 3171.144 kb on - strand, within Pf1N1B4_2826 at 3171.144 kb on - strand, within Pf1N1B4_2826 at 3171.144 kb on - strand, within Pf1N1B4_2826 at 3171.149 kb on + strand, within Pf1N1B4_2826 at 3171.149 kb on + strand, within Pf1N1B4_2826 at 3171.150 kb on - strand, within Pf1N1B4_2826 at 3171.150 kb on - strand, within Pf1N1B4_2826 at 3171.150 kb on - strand, within Pf1N1B4_2826 at 3171.150 kb on - strand, within Pf1N1B4_2826 at 3171.329 kb on - strand, within Pf1N1B4_2826 at 3171.329 kb on - strand, within Pf1N1B4_2826 at 3171.522 kb on + strand, within Pf1N1B4_2826 at 3171.522 kb on + strand, within Pf1N1B4_2826 at 3171.523 kb on - strand, within Pf1N1B4_2826 at 3171.523 kb on - strand, within Pf1N1B4_2826 at 3171.523 kb on - strand, within Pf1N1B4_2826 at 3171.523 kb on - strand, within Pf1N1B4_2826
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 3,169,175 - Pf1N1B4_2824 0.83 -1.6 3,169,438 + -2.8 3,169,457 + -1.3 3,169,547 + -0.1 3,169,547 + -1.8 3,169,691 + -1.5 3,169,753 + -0.3 3,169,753 + +2.5 3,170,069 + -0.5 3,170,072 + -1.1 3,170,072 + -1.1 3,170,072 + +1.2 3,170,176 + -2.0 3,170,176 + +0.8 3,170,176 + -1.6 3,170,176 + -1.1 3,170,177 - -1.2 3,170,177 - +1.6 3,170,177 - -1.5 3,170,177 - +1.8 3,170,180 + +0.2 3,170,180 + +1.1 3,170,180 + +0.5 3,170,180 + -1.1 3,170,181 - -1.9 3,170,181 - +0.7 3,170,181 - -2.0 3,170,181 - -0.3 3,170,181 - +1.5 3,170,236 + Pf1N1B4_2825 0.15 -2.2 3,170,237 - Pf1N1B4_2825 0.15 -0.9 3,170,237 - Pf1N1B4_2825 0.15 +1.6 3,170,237 - Pf1N1B4_2825 0.15 +0.5 3,170,237 - Pf1N1B4_2825 0.15 +1.7 3,170,237 - Pf1N1B4_2825 0.15 -0.2 3,170,237 - Pf1N1B4_2825 0.15 +1.1 3,170,237 - Pf1N1B4_2825 0.15 -1.9 3,170,418 - Pf1N1B4_2825 0.60 -0.6 3,170,418 - Pf1N1B4_2825 0.60 +2.5 3,170,418 - Pf1N1B4_2825 0.60 -1.4 3,170,461 + Pf1N1B4_2825 0.71 +3.9 3,170,462 - Pf1N1B4_2825 0.71 +1.5 3,170,462 - Pf1N1B4_2825 0.71 +0.6 3,170,562 - -0.1 3,170,577 + -0.6 3,170,578 - +1.0 3,170,578 - +0.2 3,170,578 - +2.1 3,170,699 - -0.3 3,170,724 + +1.4 3,170,724 + -0.5 3,170,724 + +1.1 3,170,724 + -1.8 3,170,753 + +1.2 3,170,753 + -1.1 3,170,753 + +0.5 3,170,754 - +0.3 3,170,869 + +1.1 3,170,899 + -0.6 3,170,903 + +0.7 3,170,904 - +1.3 3,170,904 - +0.6 3,170,919 - +0.5 3,170,919 - -2.1 3,170,996 + +0.2 3,170,996 + -1.9 3,170,997 - -0.3 3,170,998 + -1.5 3,170,998 + +0.8 3,170,998 + -1.2 3,170,998 + +0.2 3,170,999 - -1.7 3,170,999 - +1.4 3,170,999 - +0.2 3,170,999 - +0.0 3,170,999 - -0.5 3,171,020 + -0.2 3,171,028 + -0.4 3,171,029 - -0.7 3,171,033 - -1.8 3,171,033 - +0.9 3,171,033 - -0.9 3,171,134 + Pf1N1B4_2826 0.17 -1.5 3,171,135 - Pf1N1B4_2826 0.17 +2.3 3,171,135 - Pf1N1B4_2826 0.17 -0.1 3,171,143 + Pf1N1B4_2826 0.17 -0.1 3,171,144 - Pf1N1B4_2826 0.17 -1.4 3,171,144 - Pf1N1B4_2826 0.17 -2.0 3,171,144 - Pf1N1B4_2826 0.17 -2.1 3,171,149 + Pf1N1B4_2826 0.18 +0.6 3,171,149 + Pf1N1B4_2826 0.18 -1.7 3,171,150 - Pf1N1B4_2826 0.18 -1.3 3,171,150 - Pf1N1B4_2826 0.18 -0.6 3,171,150 - Pf1N1B4_2826 0.18 -1.7 3,171,150 - Pf1N1B4_2826 0.18 +0.9 3,171,329 - Pf1N1B4_2826 0.32 +0.8 3,171,329 - Pf1N1B4_2826 0.32 -1.5 3,171,522 + Pf1N1B4_2826 0.47 +1.6 3,171,522 + Pf1N1B4_2826 0.47 +0.6 3,171,523 - Pf1N1B4_2826 0.47 -2.8 3,171,523 - Pf1N1B4_2826 0.47 +1.8 3,171,523 - Pf1N1B4_2826 0.47 -1.3 3,171,523 - Pf1N1B4_2826 0.47 -1.8
Or see this region's nucleotide sequence