Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2425 and Pf1N1B4_2426 are separated by 114 nucleotides Pf1N1B4_2426 and Pf1N1B4_2427 are separated by 372 nucleotides Pf1N1B4_2427 and Pf1N1B4_2428 are separated by 13 nucleotides Pf1N1B4_2428 and Pf1N1B4_2429 are separated by 142 nucleotides
Pf1N1B4_2425 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 2,750,518 to 2,751,621
_2425
Pf1N1B4_2426 - FIG00953780: hypothetical protein, at 2,751,736 to 2,751,999
_2426
Pf1N1B4_2427 - Gamma-butyrobetaine,2-oxoglutarate dioxygenase (EC 1.14.11.1), at 2,752,372 to 2,753,535
_2427
Pf1N1B4_2428 - FIG143042: Thioesterase-like protein, at 2,753,549 to 2,754,025
_2428
Pf1N1B4_2429 - Carnitine 3-dehydrogenase (EC 1.1.1.108) (from data) , at 2,754,168 to 2,755,133
_2429
Position (kb)
2752
2753
2754 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2751.410 kb on + strand, within Pf1N1B4_2425 at 2751.458 kb on - strand, within Pf1N1B4_2425 at 2751.611 kb on + strand at 2751.686 kb on + strand at 2751.686 kb on + strand at 2751.686 kb on + strand at 2751.687 kb on - strand at 2751.687 kb on - strand at 2751.689 kb on - strand at 2751.734 kb on + strand at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.833 kb on + strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.834 kb on - strand, within Pf1N1B4_2426 at 2751.849 kb on + strand, within Pf1N1B4_2426 at 2751.849 kb on + strand, within Pf1N1B4_2426 at 2751.850 kb on - strand, within Pf1N1B4_2426 at 2751.953 kb on - strand, within Pf1N1B4_2426 at 2752.092 kb on - strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.095 kb on + strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.096 kb on - strand at 2752.373 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.421 kb on + strand at 2752.422 kb on - strand at 2752.422 kb on - strand at 2752.422 kb on - strand at 2752.422 kb on - strand at 2752.422 kb on - strand at 2752.923 kb on - strand, within Pf1N1B4_2427 at 2753.143 kb on + strand, within Pf1N1B4_2427 at 2753.144 kb on - strand, within Pf1N1B4_2427 at 2753.210 kb on + strand, within Pf1N1B4_2427 at 2753.210 kb on + strand, within Pf1N1B4_2427 at 2753.211 kb on - strand, within Pf1N1B4_2427 at 2753.366 kb on + strand, within Pf1N1B4_2427 at 2753.550 kb on + strand at 2753.684 kb on + strand, within Pf1N1B4_2428 at 2753.684 kb on + strand, within Pf1N1B4_2428 at 2753.684 kb on + strand at 2753.684 kb on + strand, within Pf1N1B4_2428 at 2753.685 kb on - strand, within Pf1N1B4_2428 at 2753.751 kb on + strand, within Pf1N1B4_2428 at 2753.832 kb on + strand, within Pf1N1B4_2428 at 2753.832 kb on + strand, within Pf1N1B4_2428 at 2753.832 kb on + strand, within Pf1N1B4_2428 at 2753.832 kb on + strand, within Pf1N1B4_2428 at 2753.833 kb on - strand, within Pf1N1B4_2428 at 2753.928 kb on + strand, within Pf1N1B4_2428 at 2753.946 kb on + strand, within Pf1N1B4_2428 at 2753.946 kb on + strand, within Pf1N1B4_2428 at 2753.946 kb on + strand, within Pf1N1B4_2428 at 2753.946 kb on + strand, within Pf1N1B4_2428 at 2753.946 kb on + strand, within Pf1N1B4_2428 at 2753.947 kb on - strand, within Pf1N1B4_2428 at 2753.947 kb on - strand, within Pf1N1B4_2428 at 2753.947 kb on - strand at 2754.006 kb on + strand at 2754.006 kb on + strand at 2754.007 kb on - strand at 2754.007 kb on - strand at 2754.007 kb on - strand at 2754.159 kb on + strand at 2754.159 kb on + strand at 2754.346 kb on + strand, within Pf1N1B4_2429 at 2754.346 kb on + strand, within Pf1N1B4_2429 at 2754.346 kb on + strand, within Pf1N1B4_2429 at 2754.346 kb on + strand, within Pf1N1B4_2429 at 2754.347 kb on - strand, within Pf1N1B4_2429 at 2754.347 kb on - strand, within Pf1N1B4_2429 at 2754.424 kb on + strand, within Pf1N1B4_2429 at 2754.424 kb on + strand, within Pf1N1B4_2429 at 2754.424 kb on + strand, within Pf1N1B4_2429 at 2754.425 kb on - strand, within Pf1N1B4_2429 at 2754.425 kb on - strand, within Pf1N1B4_2429 at 2754.425 kb on - strand, within Pf1N1B4_2429 at 2754.425 kb on - strand, within Pf1N1B4_2429 at 2754.438 kb on - strand, within Pf1N1B4_2429
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 2,751,410 + Pf1N1B4_2425 0.81 +0.0 2,751,458 - Pf1N1B4_2425 0.85 -1.0 2,751,611 + +0.3 2,751,686 + -0.9 2,751,686 + +4.4 2,751,686 + +1.8 2,751,687 - -0.4 2,751,687 - +2.6 2,751,689 - +1.2 2,751,734 + +0.2 2,751,833 + Pf1N1B4_2426 0.37 +0.3 2,751,833 + Pf1N1B4_2426 0.37 -0.1 2,751,833 + Pf1N1B4_2426 0.37 +0.4 2,751,833 + Pf1N1B4_2426 0.37 +0.8 2,751,833 + Pf1N1B4_2426 0.37 +1.0 2,751,834 - Pf1N1B4_2426 0.37 -0.5 2,751,834 - Pf1N1B4_2426 0.37 -1.3 2,751,834 - Pf1N1B4_2426 0.37 -0.4 2,751,834 - Pf1N1B4_2426 0.37 +2.4 2,751,834 - Pf1N1B4_2426 0.37 +2.5 2,751,834 - Pf1N1B4_2426 0.37 +1.5 2,751,849 + Pf1N1B4_2426 0.43 +0.8 2,751,849 + Pf1N1B4_2426 0.43 +2.1 2,751,850 - Pf1N1B4_2426 0.43 +2.0 2,751,953 - Pf1N1B4_2426 0.82 +1.2 2,752,092 - +1.2 2,752,095 + +2.4 2,752,095 + -2.0 2,752,095 + +2.2 2,752,095 + -0.7 2,752,095 + -0.5 2,752,095 + +1.9 2,752,095 + -0.2 2,752,096 - +1.3 2,752,096 - -2.3 2,752,096 - +1.0 2,752,096 - -1.6 2,752,096 - +1.0 2,752,096 - +1.1 2,752,096 - +2.1 2,752,096 - +0.9 2,752,096 - -1.3 2,752,373 + -2.7 2,752,421 + +0.6 2,752,421 + +0.5 2,752,421 + -1.0 2,752,421 + +0.3 2,752,421 + +0.1 2,752,421 + -0.9 2,752,421 + +1.0 2,752,422 - -0.6 2,752,422 - -1.5 2,752,422 - +3.2 2,752,422 - +1.1 2,752,422 - +0.3 2,752,923 - Pf1N1B4_2427 0.47 +2.7 2,753,143 + Pf1N1B4_2427 0.66 -0.3 2,753,144 - Pf1N1B4_2427 0.66 +0.6 2,753,210 + Pf1N1B4_2427 0.72 +0.9 2,753,210 + Pf1N1B4_2427 0.72 +1.6 2,753,211 - Pf1N1B4_2427 0.72 +0.3 2,753,366 + Pf1N1B4_2427 0.85 +0.9 2,753,550 + -0.1 2,753,684 + Pf1N1B4_2428 0.28 +0.4 2,753,684 + Pf1N1B4_2428 0.28 +1.7 2,753,684 + +1.0 2,753,684 + Pf1N1B4_2428 0.28 -1.4 2,753,685 - Pf1N1B4_2428 0.29 +0.0 2,753,751 + Pf1N1B4_2428 0.42 -1.0 2,753,832 + Pf1N1B4_2428 0.59 -2.7 2,753,832 + Pf1N1B4_2428 0.59 -1.7 2,753,832 + Pf1N1B4_2428 0.59 +0.2 2,753,832 + Pf1N1B4_2428 0.59 +0.6 2,753,833 - Pf1N1B4_2428 0.60 +0.8 2,753,928 + Pf1N1B4_2428 0.79 -0.6 2,753,946 + Pf1N1B4_2428 0.83 +0.9 2,753,946 + Pf1N1B4_2428 0.83 +0.6 2,753,946 + Pf1N1B4_2428 0.83 -2.2 2,753,946 + Pf1N1B4_2428 0.83 -1.9 2,753,946 + Pf1N1B4_2428 0.83 +4.6 2,753,947 - Pf1N1B4_2428 0.83 -1.0 2,753,947 - Pf1N1B4_2428 0.83 -0.6 2,753,947 - +1.5 2,754,006 + +0.2 2,754,006 + -0.7 2,754,007 - -1.5 2,754,007 - -0.6 2,754,007 - +1.6 2,754,159 + +0.1 2,754,159 + +0.2 2,754,346 + Pf1N1B4_2429 0.18 +1.3 2,754,346 + Pf1N1B4_2429 0.18 +1.2 2,754,346 + Pf1N1B4_2429 0.18 +0.5 2,754,346 + Pf1N1B4_2429 0.18 +2.5 2,754,347 - Pf1N1B4_2429 0.19 -0.7 2,754,347 - Pf1N1B4_2429 0.19 -1.7 2,754,424 + Pf1N1B4_2429 0.27 -1.0 2,754,424 + Pf1N1B4_2429 0.27 +3.0 2,754,424 + Pf1N1B4_2429 0.27 +2.2 2,754,425 - Pf1N1B4_2429 0.27 -1.4 2,754,425 - Pf1N1B4_2429 0.27 +0.1 2,754,425 - Pf1N1B4_2429 0.27 +1.3 2,754,425 - Pf1N1B4_2429 0.27 -0.2 2,754,438 - Pf1N1B4_2429 0.28 +0.6
Or see this region's nucleotide sequence