Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2362

Experiment: carbon source Sucrose 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2361 and Pf1N1B4_2362 overlap by 1 nucleotidesPf1N1B4_2362 and Pf1N1B4_2363 overlap by 1 nucleotidesPf1N1B4_2363 and Pf1N1B4_2364 overlap by 8 nucleotides Pf1N1B4_2361 - Malonate decarboxylase alpha subunit, at 2,678,613 to 2,680,211 _2361 Pf1N1B4_2362 - Triphosphoribosyl-dephospho-CoA synthetase (EC 2.7.8.25), at 2,680,211 to 2,681,086 _2362 Pf1N1B4_2363 - Malonate decarboxylase delta subunit, at 2,681,086 to 2,681,385 _2363 Pf1N1B4_2364 - Malonate decarboxylase beta subunit, at 2,681,378 to 2,682,229 _2364 Position (kb) 2680 2681 2682Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2679.286 kb on - strand, within Pf1N1B4_2361at 2679.286 kb on - strand, within Pf1N1B4_2361at 2679.286 kb on - strand, within Pf1N1B4_2361at 2679.363 kb on + strand, within Pf1N1B4_2361at 2679.363 kb on + strand, within Pf1N1B4_2361at 2679.364 kb on - strand, within Pf1N1B4_2361at 2679.364 kb on - strand, within Pf1N1B4_2361at 2679.364 kb on - strand, within Pf1N1B4_2361at 2679.364 kb on - strand, within Pf1N1B4_2361at 2679.501 kb on + strand, within Pf1N1B4_2361at 2679.501 kb on + strand, within Pf1N1B4_2361at 2679.502 kb on - strand, within Pf1N1B4_2361at 2679.502 kb on - strand, within Pf1N1B4_2361at 2679.910 kb on - strandat 2679.954 kb on + strandat 2679.955 kb on - strand, within Pf1N1B4_2361at 2679.960 kb on + strand, within Pf1N1B4_2361at 2679.960 kb on + strand, within Pf1N1B4_2361at 2679.960 kb on + strand, within Pf1N1B4_2361at 2679.960 kb on + strand, within Pf1N1B4_2361at 2679.960 kb on + strand, within Pf1N1B4_2361at 2679.960 kb on + strand, within Pf1N1B4_2361at 2679.961 kb on - strand, within Pf1N1B4_2361at 2680.038 kb on - strand, within Pf1N1B4_2361at 2680.179 kb on + strandat 2680.179 kb on + strandat 2680.179 kb on + strandat 2680.179 kb on + strandat 2680.180 kb on - strandat 2680.180 kb on - strandat 2680.180 kb on - strandat 2680.180 kb on - strandat 2680.180 kb on - strandat 2680.180 kb on - strandat 2680.180 kb on - strandat 2680.356 kb on + strand, within Pf1N1B4_2362at 2680.356 kb on + strand, within Pf1N1B4_2362at 2680.356 kb on + strand, within Pf1N1B4_2362at 2680.357 kb on - strand, within Pf1N1B4_2362at 2680.357 kb on - strand, within Pf1N1B4_2362at 2680.357 kb on - strand, within Pf1N1B4_2362at 2680.449 kb on + strand, within Pf1N1B4_2362at 2680.526 kb on - strand, within Pf1N1B4_2362at 2680.526 kb on - strand, within Pf1N1B4_2362at 2680.591 kb on + strand, within Pf1N1B4_2362at 2680.592 kb on - strand, within Pf1N1B4_2362at 2680.592 kb on - strand, within Pf1N1B4_2362at 2680.665 kb on + strand, within Pf1N1B4_2362at 2680.666 kb on - strand, within Pf1N1B4_2362at 2680.981 kb on - strand, within Pf1N1B4_2362at 2681.410 kb on + strandat 2681.410 kb on + strandat 2681.410 kb on + strandat 2681.410 kb on + strandat 2681.410 kb on + strandat 2681.411 kb on - strandat 2681.411 kb on - strandat 2681.411 kb on - strandat 2681.411 kb on - strandat 2681.411 kb on - strandat 2681.411 kb on - strandat 2681.411 kb on - strandat 2681.411 kb on - strandat 2681.891 kb on + strand, within Pf1N1B4_2364at 2681.891 kb on + strand, within Pf1N1B4_2364at 2681.891 kb on + strand, within Pf1N1B4_2364at 2681.892 kb on - strand, within Pf1N1B4_2364at 2681.892 kb on - strand, within Pf1N1B4_2364at 2681.969 kb on + strand, within Pf1N1B4_2364at 2681.969 kb on + strand, within Pf1N1B4_2364at 2681.970 kb on - strand, within Pf1N1B4_2364at 2681.970 kb on - strand, within Pf1N1B4_2364at 2682.047 kb on + strand, within Pf1N1B4_2364at 2682.047 kb on + strand, within Pf1N1B4_2364

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM
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2,679,286 - Pf1N1B4_2361 0.42 +1.3
2,679,286 - Pf1N1B4_2361 0.42 -0.4
2,679,286 - Pf1N1B4_2361 0.42 -1.1
2,679,363 + Pf1N1B4_2361 0.47 +0.8
2,679,363 + Pf1N1B4_2361 0.47 +0.9
2,679,364 - Pf1N1B4_2361 0.47 +1.1
2,679,364 - Pf1N1B4_2361 0.47 -0.7
2,679,364 - Pf1N1B4_2361 0.47 +1.2
2,679,364 - Pf1N1B4_2361 0.47 -1.2
2,679,501 + Pf1N1B4_2361 0.56 -1.5
2,679,501 + Pf1N1B4_2361 0.56 +1.4
2,679,502 - Pf1N1B4_2361 0.56 -0.8
2,679,502 - Pf1N1B4_2361 0.56 -1.3
2,679,910 - -0.0
2,679,954 + -0.7
2,679,955 - Pf1N1B4_2361 0.84 +0.7
2,679,960 + Pf1N1B4_2361 0.84 -0.7
2,679,960 + Pf1N1B4_2361 0.84 -2.7
2,679,960 + Pf1N1B4_2361 0.84 -2.3
2,679,960 + Pf1N1B4_2361 0.84 +0.8
2,679,960 + Pf1N1B4_2361 0.84 +1.2
2,679,960 + Pf1N1B4_2361 0.84 -0.2
2,679,961 - Pf1N1B4_2361 0.84 -2.2
2,680,038 - Pf1N1B4_2361 0.89 +0.7
2,680,179 + +2.6
2,680,179 + -0.1
2,680,179 + +0.5
2,680,179 + -2.2
2,680,180 - -1.7
2,680,180 - -1.7
2,680,180 - -0.7
2,680,180 - +0.6
2,680,180 - +0.8
2,680,180 - +2.7
2,680,180 - -2.3
2,680,356 + Pf1N1B4_2362 0.17 +0.2
2,680,356 + Pf1N1B4_2362 0.17 -0.4
2,680,356 + Pf1N1B4_2362 0.17 -0.6
2,680,357 - Pf1N1B4_2362 0.17 +2.0
2,680,357 - Pf1N1B4_2362 0.17 +1.0
2,680,357 - Pf1N1B4_2362 0.17 -2.8
2,680,449 + Pf1N1B4_2362 0.27 +0.9
2,680,526 - Pf1N1B4_2362 0.36 +0.5
2,680,526 - Pf1N1B4_2362 0.36 -1.4
2,680,591 + Pf1N1B4_2362 0.43 +3.3
2,680,592 - Pf1N1B4_2362 0.43 +1.8
2,680,592 - Pf1N1B4_2362 0.43 +0.8
2,680,665 + Pf1N1B4_2362 0.52 -1.6
2,680,666 - Pf1N1B4_2362 0.52 +0.1
2,680,981 - Pf1N1B4_2362 0.88 +1.6
2,681,410 + +1.7
2,681,410 + +0.1
2,681,410 + -1.7
2,681,410 + -0.2
2,681,410 + +0.7
2,681,411 - +1.1
2,681,411 - +1.3
2,681,411 - -0.7
2,681,411 - -3.0
2,681,411 - +0.9
2,681,411 - +1.2
2,681,411 - +1.4
2,681,411 - +1.3
2,681,891 + Pf1N1B4_2364 0.60 -1.8
2,681,891 + Pf1N1B4_2364 0.60 +2.0
2,681,891 + Pf1N1B4_2364 0.60 -3.1
2,681,892 - Pf1N1B4_2364 0.60 -1.9
2,681,892 - Pf1N1B4_2364 0.60 +0.4
2,681,969 + Pf1N1B4_2364 0.69 +0.9
2,681,969 + Pf1N1B4_2364 0.69 -2.9
2,681,970 - Pf1N1B4_2364 0.69 +0.3
2,681,970 - Pf1N1B4_2364 0.69 -2.5
2,682,047 + Pf1N1B4_2364 0.79 +0.8
2,682,047 + Pf1N1B4_2364 0.79 +2.7

Or see this region's nucleotide sequence