Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1500 and Pf1N1B4_1501 are separated by 470 nucleotides Pf1N1B4_1501 and Pf1N1B4_1502 overlap by 25 nucleotides Pf1N1B4_1502 and Pf1N1B4_1503 overlap by 20 nucleotides
Pf1N1B4_1500 - Chromosome (plasmid) partitioning protein ParA, at 1,734,188 to 1,734,958
_1500
Pf1N1B4_1501 - 3-oxoacyl-[ACP] synthase, at 1,735,429 to 1,736,043
_1501
Pf1N1B4_1502 - FIG018329: 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 1,736,019 to 1,736,828
_1502
Pf1N1B4_1503 - Acyl carrier protein (ACP1), at 1,736,809 to 1,737,069
_1503
Position (kb)
1735
1736
1737 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1734.576 kb on + strand, within Pf1N1B4_1500 at 1734.576 kb on + strand, within Pf1N1B4_1500 at 1734.588 kb on + strand, within Pf1N1B4_1500 at 1734.717 kb on + strand, within Pf1N1B4_1500 at 1734.717 kb on + strand, within Pf1N1B4_1500 at 1734.717 kb on + strand, within Pf1N1B4_1500 at 1734.718 kb on - strand, within Pf1N1B4_1500 at 1734.718 kb on - strand, within Pf1N1B4_1500 at 1734.822 kb on + strand, within Pf1N1B4_1500 at 1734.975 kb on - strand at 1734.994 kb on + strand at 1734.994 kb on + strand at 1734.994 kb on + strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.995 kb on - strand at 1734.998 kb on - strand at 1734.998 kb on - strand at 1734.998 kb on - strand at 1735.014 kb on + strand at 1735.015 kb on - strand at 1735.015 kb on - strand at 1735.015 kb on - strand at 1735.019 kb on - strand at 1735.019 kb on - strand at 1735.019 kb on - strand at 1735.019 kb on - strand at 1735.039 kb on - strand at 1735.053 kb on - strand at 1735.053 kb on - strand at 1735.189 kb on - strand at 1735.255 kb on - strand at 1735.255 kb on - strand at 1735.255 kb on - strand at 1735.255 kb on - strand at 1735.541 kb on + strand, within Pf1N1B4_1501 at 1735.541 kb on + strand, within Pf1N1B4_1501 at 1735.554 kb on + strand, within Pf1N1B4_1501 at 1735.554 kb on + strand, within Pf1N1B4_1501 at 1735.554 kb on + strand, within Pf1N1B4_1501 at 1735.555 kb on - strand, within Pf1N1B4_1501 at 1735.555 kb on - strand, within Pf1N1B4_1501 at 1735.748 kb on + strand, within Pf1N1B4_1501 at 1735.748 kb on + strand, within Pf1N1B4_1501 at 1735.749 kb on - strand, within Pf1N1B4_1501 at 1735.749 kb on - strand, within Pf1N1B4_1501 at 1735.749 kb on - strand, within Pf1N1B4_1501 at 1735.787 kb on + strand, within Pf1N1B4_1501 at 1735.797 kb on - strand, within Pf1N1B4_1501 at 1735.826 kb on - strand, within Pf1N1B4_1501 at 1735.826 kb on - strand, within Pf1N1B4_1501 at 1735.969 kb on - strand, within Pf1N1B4_1501 at 1736.018 kb on + strand at 1736.019 kb on - strand at 1736.019 kb on - strand at 1736.068 kb on - strand at 1736.122 kb on + strand, within Pf1N1B4_1502 at 1736.262 kb on + strand, within Pf1N1B4_1502 at 1736.262 kb on + strand, within Pf1N1B4_1502 at 1736.262 kb on + strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.263 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.437 kb on - strand, within Pf1N1B4_1502 at 1736.462 kb on + strand, within Pf1N1B4_1502 at 1736.710 kb on + strand, within Pf1N1B4_1502 at 1736.710 kb on + strand, within Pf1N1B4_1502 at 1736.710 kb on + strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.711 kb on - strand, within Pf1N1B4_1502 at 1736.772 kb on + strand at 1736.772 kb on + strand at 1736.773 kb on - strand at 1736.773 kb on - strand at 1736.773 kb on - strand at 1736.773 kb on - strand at 1736.784 kb on + strand at 1736.784 kb on + strand at 1736.784 kb on + strand at 1736.784 kb on + strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.785 kb on - strand at 1736.789 kb on + strand at 1736.789 kb on + strand at 1736.790 kb on - strand at 1736.790 kb on - strand at 1736.790 kb on - strand at 1736.790 kb on - strand at 1736.793 kb on - strand at 1736.972 kb on - strand, within Pf1N1B4_1503 at 1737.010 kb on - strand, within Pf1N1B4_1503
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 1,734,576 + Pf1N1B4_1500 0.50 -2.1 1,734,576 + Pf1N1B4_1500 0.50 +1.5 1,734,588 + Pf1N1B4_1500 0.52 +0.0 1,734,717 + Pf1N1B4_1500 0.69 -1.4 1,734,717 + Pf1N1B4_1500 0.69 -2.2 1,734,717 + Pf1N1B4_1500 0.69 +0.3 1,734,718 - Pf1N1B4_1500 0.69 -1.5 1,734,718 - Pf1N1B4_1500 0.69 -0.7 1,734,822 + Pf1N1B4_1500 0.82 +1.5 1,734,975 - +1.3 1,734,994 + +0.5 1,734,994 + -1.7 1,734,994 + +0.5 1,734,995 - +2.1 1,734,995 - -1.9 1,734,995 - +0.5 1,734,995 - -1.8 1,734,995 - +1.0 1,734,995 - +2.6 1,734,995 - -1.5 1,734,995 - -2.0 1,734,998 - +3.3 1,734,998 - +0.6 1,734,998 - -1.2 1,735,014 + +0.7 1,735,015 - -0.5 1,735,015 - +1.2 1,735,015 - +1.5 1,735,019 - -3.0 1,735,019 - -0.1 1,735,019 - +0.1 1,735,019 - +1.6 1,735,039 - +2.1 1,735,053 - +1.0 1,735,053 - -0.1 1,735,189 - +2.2 1,735,255 - -1.3 1,735,255 - -0.3 1,735,255 - +1.0 1,735,255 - -1.6 1,735,541 + Pf1N1B4_1501 0.18 +1.7 1,735,541 + Pf1N1B4_1501 0.18 -1.0 1,735,554 + Pf1N1B4_1501 0.20 -0.3 1,735,554 + Pf1N1B4_1501 0.20 +0.3 1,735,554 + Pf1N1B4_1501 0.20 +2.1 1,735,555 - Pf1N1B4_1501 0.20 +0.7 1,735,555 - Pf1N1B4_1501 0.20 -1.1 1,735,748 + Pf1N1B4_1501 0.52 -1.8 1,735,748 + Pf1N1B4_1501 0.52 -2.2 1,735,749 - Pf1N1B4_1501 0.52 -0.9 1,735,749 - Pf1N1B4_1501 0.52 +1.0 1,735,749 - Pf1N1B4_1501 0.52 +1.0 1,735,787 + Pf1N1B4_1501 0.58 +1.2 1,735,797 - Pf1N1B4_1501 0.60 +1.1 1,735,826 - Pf1N1B4_1501 0.65 +0.9 1,735,826 - Pf1N1B4_1501 0.65 +0.9 1,735,969 - Pf1N1B4_1501 0.88 +2.2 1,736,018 + +1.2 1,736,019 - +2.5 1,736,019 - -0.8 1,736,068 - -2.3 1,736,122 + Pf1N1B4_1502 0.13 -1.5 1,736,262 + Pf1N1B4_1502 0.30 -0.9 1,736,262 + Pf1N1B4_1502 0.30 -1.3 1,736,262 + Pf1N1B4_1502 0.30 -0.1 1,736,263 - Pf1N1B4_1502 0.30 +0.8 1,736,263 - Pf1N1B4_1502 0.30 +1.2 1,736,263 - Pf1N1B4_1502 0.30 +0.1 1,736,263 - Pf1N1B4_1502 0.30 -0.5 1,736,263 - Pf1N1B4_1502 0.30 +0.8 1,736,263 - Pf1N1B4_1502 0.30 +2.1 1,736,437 - Pf1N1B4_1502 0.52 +1.5 1,736,437 - Pf1N1B4_1502 0.52 -3.2 1,736,437 - Pf1N1B4_1502 0.52 +1.6 1,736,437 - Pf1N1B4_1502 0.52 +2.2 1,736,437 - Pf1N1B4_1502 0.52 -2.5 1,736,462 + Pf1N1B4_1502 0.55 -1.8 1,736,710 + Pf1N1B4_1502 0.85 +1.3 1,736,710 + Pf1N1B4_1502 0.85 -0.3 1,736,710 + Pf1N1B4_1502 0.85 +0.7 1,736,711 - Pf1N1B4_1502 0.85 -0.0 1,736,711 - Pf1N1B4_1502 0.85 +0.2 1,736,711 - Pf1N1B4_1502 0.85 -3.7 1,736,711 - Pf1N1B4_1502 0.85 +0.0 1,736,711 - Pf1N1B4_1502 0.85 -2.4 1,736,711 - Pf1N1B4_1502 0.85 -0.7 1,736,711 - Pf1N1B4_1502 0.85 -1.9 1,736,711 - Pf1N1B4_1502 0.85 +0.1 1,736,772 + -1.2 1,736,772 + -3.0 1,736,773 - -0.2 1,736,773 - +1.6 1,736,773 - +1.0 1,736,773 - +1.2 1,736,784 + -1.5 1,736,784 + -1.4 1,736,784 + +0.3 1,736,784 + +0.5 1,736,785 - -0.5 1,736,785 - -2.2 1,736,785 - -1.9 1,736,785 - +0.0 1,736,785 - -2.4 1,736,785 - -1.8 1,736,785 - +0.8 1,736,785 - +0.2 1,736,789 + +1.6 1,736,789 + +1.9 1,736,790 - +0.4 1,736,790 - -2.3 1,736,790 - -1.2 1,736,790 - -1.9 1,736,793 - +1.7 1,736,972 - Pf1N1B4_1503 0.62 -0.9 1,737,010 - Pf1N1B4_1503 0.77 +1.0
Or see this region's nucleotide sequence