Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_1339

Experiment: carbon source Sucrose 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_1338 and Pf1N1B4_1339 are separated by 540 nucleotidesPf1N1B4_1339 and Pf1N1B4_1340 overlap by 4 nucleotidesPf1N1B4_1340 and Pf1N1B4_1341 overlap by 4 nucleotides Pf1N1B4_1338 - Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB, at 1,533,225 to 1,534,601 _1338 Pf1N1B4_1339 - ABC transporter (iron.B12.siderophore.hemin) , ATP-binding component, at 1,535,142 to 1,536,113 _1339 Pf1N1B4_1340 - ABC transporter (iron.B12.siderophore.hemin) , periplasmic substrate-binding component, at 1,536,110 to 1,537,057 _1340 Pf1N1B4_1341 - ABC transporter (iron.B12.siderophore.hemin) , permease component, at 1,537,054 to 1,538,064 _1341 Position (kb) 1535 1536 1537Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1534.263 kb on + strand, within Pf1N1B4_1338at 1534.264 kb on - strand, within Pf1N1B4_1338at 1534.264 kb on - strand, within Pf1N1B4_1338at 1534.264 kb on - strand, within Pf1N1B4_1338at 1534.264 kb on - strand, within Pf1N1B4_1338at 1534.572 kb on - strandat 1534.572 kb on - strandat 1534.645 kb on - strandat 1534.826 kb on + strandat 1534.921 kb on + strandat 1534.921 kb on + strandat 1534.922 kb on - strandat 1534.922 kb on - strandat 1534.922 kb on - strandat 1534.961 kb on - strandat 1534.961 kb on - strandat 1534.961 kb on - strandat 1534.984 kb on + strandat 1534.985 kb on - strandat 1534.985 kb on - strandat 1535.025 kb on + strandat 1535.026 kb on - strandat 1535.032 kb on + strandat 1535.032 kb on + strandat 1535.032 kb on + strandat 1535.032 kb on + strandat 1535.130 kb on - strandat 1535.139 kb on - strandat 1535.139 kb on - strandat 1535.139 kb on - strandat 1535.536 kb on - strand, within Pf1N1B4_1339at 1535.536 kb on - strand, within Pf1N1B4_1339at 1535.961 kb on + strand, within Pf1N1B4_1339at 1535.961 kb on + strand, within Pf1N1B4_1339at 1535.961 kb on + strand, within Pf1N1B4_1339at 1535.962 kb on - strand, within Pf1N1B4_1339at 1535.962 kb on - strand, within Pf1N1B4_1339at 1535.962 kb on - strand, within Pf1N1B4_1339at 1536.037 kb on + strandat 1536.037 kb on + strandat 1536.037 kb on + strandat 1536.037 kb on + strandat 1536.038 kb on - strandat 1536.392 kb on + strandat 1536.393 kb on - strand, within Pf1N1B4_1340at 1536.393 kb on - strand, within Pf1N1B4_1340at 1536.515 kb on + strand, within Pf1N1B4_1340at 1536.516 kb on - strand, within Pf1N1B4_1340at 1536.578 kb on + strand, within Pf1N1B4_1340at 1536.578 kb on + strand, within Pf1N1B4_1340at 1536.579 kb on - strand, within Pf1N1B4_1340at 1536.881 kb on + strand, within Pf1N1B4_1340at 1536.983 kb on + strandat 1536.983 kb on + strandat 1536.984 kb on - strandat 1536.984 kb on - strandat 1536.984 kb on - strandat 1536.984 kb on - strandat 1537.069 kb on + strandat 1537.070 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM
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1,534,263 + Pf1N1B4_1338 0.75 -2.2
1,534,264 - Pf1N1B4_1338 0.75 +1.4
1,534,264 - Pf1N1B4_1338 0.75 -0.8
1,534,264 - Pf1N1B4_1338 0.75 -2.1
1,534,264 - Pf1N1B4_1338 0.75 +0.4
1,534,572 - -1.1
1,534,572 - +0.9
1,534,645 - +1.4
1,534,826 + -0.4
1,534,921 + -3.0
1,534,921 + -0.2
1,534,922 - +0.9
1,534,922 - -1.8
1,534,922 - +0.4
1,534,961 - +0.4
1,534,961 - +0.2
1,534,961 - +0.5
1,534,984 + -1.7
1,534,985 - +0.8
1,534,985 - -1.5
1,535,025 + -0.7
1,535,026 - +3.0
1,535,032 + +1.2
1,535,032 + +0.2
1,535,032 + -2.0
1,535,032 + -3.7
1,535,130 - -0.6
1,535,139 - +0.6
1,535,139 - +1.6
1,535,139 - -1.3
1,535,536 - Pf1N1B4_1339 0.41 -0.9
1,535,536 - Pf1N1B4_1339 0.41 -1.4
1,535,961 + Pf1N1B4_1339 0.84 +2.3
1,535,961 + Pf1N1B4_1339 0.84 -1.7
1,535,961 + Pf1N1B4_1339 0.84 +2.3
1,535,962 - Pf1N1B4_1339 0.84 +3.4
1,535,962 - Pf1N1B4_1339 0.84 -1.1
1,535,962 - Pf1N1B4_1339 0.84 +1.1
1,536,037 + -0.6
1,536,037 + -0.9
1,536,037 + -0.9
1,536,037 + +1.4
1,536,038 - +0.4
1,536,392 + -0.1
1,536,393 - Pf1N1B4_1340 0.30 -0.1
1,536,393 - Pf1N1B4_1340 0.30 -2.4
1,536,515 + Pf1N1B4_1340 0.43 -2.6
1,536,516 - Pf1N1B4_1340 0.43 -1.6
1,536,578 + Pf1N1B4_1340 0.49 +1.4
1,536,578 + Pf1N1B4_1340 0.49 +2.9
1,536,579 - Pf1N1B4_1340 0.49 +1.2
1,536,881 + Pf1N1B4_1340 0.81 +2.4
1,536,983 + +0.4
1,536,983 + +1.7
1,536,984 - -1.8
1,536,984 - +0.4
1,536,984 - +1.4
1,536,984 - +0.4
1,537,069 + +0.0
1,537,070 - -2.2

Or see this region's nucleotide sequence