Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1309 and Pf1N1B4_1310 are separated by 326 nucleotides Pf1N1B4_1310 and Pf1N1B4_1311 are separated by 30 nucleotides
Pf1N1B4_1309 - Biotin carboxylase of acetyl-CoA carboxylase (EC 6.3.4.14), at 1,492,027 to 1,493,385
_1309
Pf1N1B4_1310 - Ribosomal protein L11 methyltransferase (EC 2.1.1.-), at 1,493,712 to 1,494,590
_1310
Pf1N1B4_1311 - Probable transmembrane protein, at 1,494,621 to 1,495,841
_1311
Position (kb)
1493
1494
1495 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1493.651 kb on - strand at 1493.689 kb on + strand at 1493.689 kb on + strand at 1493.689 kb on + strand at 1493.689 kb on + strand at 1493.690 kb on - strand at 1493.691 kb on + strand at 1493.763 kb on + strand at 1493.763 kb on + strand at 1493.763 kb on + strand at 1493.764 kb on - strand at 1494.088 kb on + strand, within Pf1N1B4_1310 at 1494.089 kb on - strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.323 kb on + strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.324 kb on - strand, within Pf1N1B4_1310 at 1494.348 kb on + strand, within Pf1N1B4_1310 at 1494.349 kb on - strand, within Pf1N1B4_1310 at 1494.385 kb on + strand, within Pf1N1B4_1310 at 1494.386 kb on - strand, within Pf1N1B4_1310 at 1494.386 kb on - strand, within Pf1N1B4_1310 at 1494.386 kb on - strand, within Pf1N1B4_1310 at 1494.516 kb on + strand at 1494.573 kb on - strand at 1494.606 kb on - strand at 1494.681 kb on - strand at 1494.734 kb on - strand at 1494.754 kb on + strand, within Pf1N1B4_1311 at 1494.755 kb on - strand, within Pf1N1B4_1311 at 1494.755 kb on - strand, within Pf1N1B4_1311 at 1495.303 kb on + strand, within Pf1N1B4_1311 at 1495.304 kb on - strand, within Pf1N1B4_1311 at 1495.426 kb on - strand, within Pf1N1B4_1311 at 1495.435 kb on - strand, within Pf1N1B4_1311
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 1,493,651 - -2.2 1,493,689 + +0.3 1,493,689 + +1.1 1,493,689 + +0.0 1,493,689 + -1.2 1,493,690 - -2.4 1,493,691 + +1.7 1,493,763 + -2.5 1,493,763 + -0.7 1,493,763 + +1.8 1,493,764 - +0.5 1,494,088 + Pf1N1B4_1310 0.43 -0.9 1,494,089 - Pf1N1B4_1310 0.43 -1.7 1,494,323 + Pf1N1B4_1310 0.70 -1.5 1,494,323 + Pf1N1B4_1310 0.70 +0.5 1,494,323 + Pf1N1B4_1310 0.70 +0.5 1,494,323 + Pf1N1B4_1310 0.70 +1.1 1,494,324 - Pf1N1B4_1310 0.70 +2.3 1,494,324 - Pf1N1B4_1310 0.70 +1.5 1,494,324 - Pf1N1B4_1310 0.70 -1.0 1,494,324 - Pf1N1B4_1310 0.70 -0.6 1,494,348 + Pf1N1B4_1310 0.72 +1.1 1,494,349 - Pf1N1B4_1310 0.72 +2.0 1,494,385 + Pf1N1B4_1310 0.77 +1.7 1,494,386 - Pf1N1B4_1310 0.77 +1.9 1,494,386 - Pf1N1B4_1310 0.77 +0.8 1,494,386 - Pf1N1B4_1310 0.77 +2.5 1,494,516 + +1.0 1,494,573 - -0.4 1,494,606 - -1.5 1,494,681 - +2.6 1,494,734 - -1.1 1,494,754 + Pf1N1B4_1311 0.11 +0.5 1,494,755 - Pf1N1B4_1311 0.11 +0.2 1,494,755 - Pf1N1B4_1311 0.11 -1.0 1,495,303 + Pf1N1B4_1311 0.56 -0.4 1,495,304 - Pf1N1B4_1311 0.56 -2.5 1,495,426 - Pf1N1B4_1311 0.66 +0.7 1,495,435 - Pf1N1B4_1311 0.67 +2.6
Or see this region's nucleotide sequence