Experiment: carbon source Sucrose 5mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1211 and Pf1N1B4_1212 are separated by 418 nucleotides Pf1N1B4_1212 and Pf1N1B4_1213 are separated by 84 nucleotides Pf1N1B4_1213 and Pf1N1B4_1214 are separated by 138 nucleotides
Pf1N1B4_1211 - Hydrogen peroxide-inducible genes activator, at 1,373,726 to 1,374,658
_1211
Pf1N1B4_1212 - Predicted flavin reductase RutF in novel pyrimidine catabolism pathway, at 1,375,077 to 1,375,379
_1212
Pf1N1B4_1213 - Transcriptional regulator, at 1,375,464 to 1,375,739
_1213
Pf1N1B4_1214 - Chorismate--pyruvate lyase (EC 4.1.3.40), at 1,375,878 to 1,376,408
_1214
Position (kb)
1375
1376 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1374.273 kb on + strand, within Pf1N1B4_1211 at 1374.273 kb on + strand, within Pf1N1B4_1211 at 1374.302 kb on + strand, within Pf1N1B4_1211 at 1374.302 kb on + strand, within Pf1N1B4_1211 at 1374.303 kb on - strand at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.303 kb on - strand at 1374.303 kb on - strand, within Pf1N1B4_1211 at 1374.338 kb on + strand, within Pf1N1B4_1211 at 1374.338 kb on + strand, within Pf1N1B4_1211 at 1374.651 kb on + strand at 1374.651 kb on + strand at 1374.651 kb on + strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.652 kb on - strand at 1374.724 kb on + strand at 1374.724 kb on + strand at 1374.725 kb on - strand at 1374.731 kb on - strand at 1374.737 kb on + strand at 1374.737 kb on + strand at 1374.738 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.760 kb on - strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.763 kb on + strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.764 kb on - strand at 1374.766 kb on + strand at 1374.767 kb on - strand at 1374.768 kb on + strand at 1374.847 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.852 kb on + strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.853 kb on - strand at 1374.872 kb on + strand at 1374.872 kb on + strand at 1374.873 kb on - strand at 1374.873 kb on - strand at 1374.897 kb on - strand at 1375.320 kb on + strand, within Pf1N1B4_1212 at 1375.321 kb on - strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.335 kb on + strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.336 kb on - strand, within Pf1N1B4_1212 at 1375.469 kb on - strand at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.494 kb on - strand, within Pf1N1B4_1213 at 1375.495 kb on + strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.496 kb on - strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.502 kb on + strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.503 kb on - strand, within Pf1N1B4_1213 at 1375.514 kb on + strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.515 kb on - strand, within Pf1N1B4_1213 at 1375.547 kb on + strand, within Pf1N1B4_1213 at 1375.717 kb on + strand at 1375.717 kb on + strand at 1375.718 kb on - strand at 1375.718 kb on - strand at 1375.817 kb on - strand at 1375.817 kb on - strand at 1375.817 kb on - strand at 1375.826 kb on + strand at 1375.994 kb on - strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.069 kb on + strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.070 kb on - strand, within Pf1N1B4_1214 at 1376.101 kb on + strand, within Pf1N1B4_1214 at 1376.293 kb on + strand, within Pf1N1B4_1214 at 1376.293 kb on + strand, within Pf1N1B4_1214 at 1376.293 kb on + strand, within Pf1N1B4_1214 at 1376.364 kb on + strand at 1376.365 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM remove 1,374,273 + Pf1N1B4_1211 0.59 +0.2 1,374,273 + Pf1N1B4_1211 0.59 +0.2 1,374,302 + Pf1N1B4_1211 0.62 +1.9 1,374,302 + Pf1N1B4_1211 0.62 -0.4 1,374,303 - +0.2 1,374,303 - Pf1N1B4_1211 0.62 +0.3 1,374,303 - Pf1N1B4_1211 0.62 +1.0 1,374,303 - Pf1N1B4_1211 0.62 +1.3 1,374,303 - Pf1N1B4_1211 0.62 +1.6 1,374,303 - Pf1N1B4_1211 0.62 +0.8 1,374,303 - Pf1N1B4_1211 0.62 +0.1 1,374,303 - Pf1N1B4_1211 0.62 -0.3 1,374,303 - -1.3 1,374,303 - Pf1N1B4_1211 0.62 -0.7 1,374,338 + Pf1N1B4_1211 0.66 +0.1 1,374,338 + Pf1N1B4_1211 0.66 +2.1 1,374,651 + +0.2 1,374,651 + -1.5 1,374,651 + -1.6 1,374,652 - -0.5 1,374,652 - +1.8 1,374,652 - -1.7 1,374,652 - +0.1 1,374,724 + +1.2 1,374,724 + -1.8 1,374,725 - -0.2 1,374,731 - -0.7 1,374,737 + +2.4 1,374,737 + +1.5 1,374,738 - +1.2 1,374,760 - +1.4 1,374,760 - +2.1 1,374,760 - +0.7 1,374,760 - +0.3 1,374,760 - -0.5 1,374,763 + -1.3 1,374,763 + +1.3 1,374,763 + +1.0 1,374,763 + -0.5 1,374,764 - -1.9 1,374,764 - -0.0 1,374,764 - +1.6 1,374,764 - +1.5 1,374,766 + -0.2 1,374,767 - +1.3 1,374,768 + +0.3 1,374,847 + +1.4 1,374,852 + -1.1 1,374,852 + +1.2 1,374,852 + +1.1 1,374,852 + -1.0 1,374,852 + -2.4 1,374,853 - +1.3 1,374,853 - +1.2 1,374,853 - +1.7 1,374,853 - +0.8 1,374,853 - -0.3 1,374,853 - +0.6 1,374,853 - +1.6 1,374,853 - -1.7 1,374,853 - -1.3 1,374,872 + +2.8 1,374,872 + +1.8 1,374,873 - +1.4 1,374,873 - +3.2 1,374,897 - +1.3 1,375,320 + Pf1N1B4_1212 0.80 +3.7 1,375,321 - Pf1N1B4_1212 0.81 +2.5 1,375,335 + Pf1N1B4_1212 0.85 +0.7 1,375,335 + Pf1N1B4_1212 0.85 +1.5 1,375,335 + Pf1N1B4_1212 0.85 -0.8 1,375,335 + Pf1N1B4_1212 0.85 +1.9 1,375,335 + Pf1N1B4_1212 0.85 +2.5 1,375,335 + Pf1N1B4_1212 0.85 +2.2 1,375,336 - Pf1N1B4_1212 0.85 +2.2 1,375,336 - Pf1N1B4_1212 0.85 +1.3 1,375,469 - +0.6 1,375,494 - Pf1N1B4_1213 0.11 -2.6 1,375,494 - Pf1N1B4_1213 0.11 +1.2 1,375,495 + Pf1N1B4_1213 0.11 +1.7 1,375,496 - Pf1N1B4_1213 0.12 -0.1 1,375,496 - Pf1N1B4_1213 0.12 -1.1 1,375,502 + Pf1N1B4_1213 0.14 +0.3 1,375,502 + Pf1N1B4_1213 0.14 -1.1 1,375,503 - Pf1N1B4_1213 0.14 -2.0 1,375,503 - Pf1N1B4_1213 0.14 -1.7 1,375,503 - Pf1N1B4_1213 0.14 -1.7 1,375,503 - Pf1N1B4_1213 0.14 -2.5 1,375,503 - Pf1N1B4_1213 0.14 -2.8 1,375,514 + Pf1N1B4_1213 0.18 +0.7 1,375,515 - Pf1N1B4_1213 0.18 -2.3 1,375,515 - Pf1N1B4_1213 0.18 +1.4 1,375,515 - Pf1N1B4_1213 0.18 -0.1 1,375,515 - Pf1N1B4_1213 0.18 +0.5 1,375,547 + Pf1N1B4_1213 0.30 -3.0 1,375,717 + -1.3 1,375,717 + +1.6 1,375,718 - -0.5 1,375,718 - +1.8 1,375,817 - -1.0 1,375,817 - +0.7 1,375,817 - +2.3 1,375,826 + -3.2 1,375,994 - Pf1N1B4_1214 0.22 -2.5 1,376,069 + Pf1N1B4_1214 0.36 -0.9 1,376,069 + Pf1N1B4_1214 0.36 -0.7 1,376,069 + Pf1N1B4_1214 0.36 +2.9 1,376,069 + Pf1N1B4_1214 0.36 +1.5 1,376,069 + Pf1N1B4_1214 0.36 +1.7 1,376,069 + Pf1N1B4_1214 0.36 +0.8 1,376,069 + Pf1N1B4_1214 0.36 +0.2 1,376,069 + Pf1N1B4_1214 0.36 +0.4 1,376,070 - Pf1N1B4_1214 0.36 -2.3 1,376,070 - Pf1N1B4_1214 0.36 +2.9 1,376,070 - Pf1N1B4_1214 0.36 -0.3 1,376,070 - Pf1N1B4_1214 0.36 -0.2 1,376,070 - Pf1N1B4_1214 0.36 -1.5 1,376,070 - Pf1N1B4_1214 0.36 +1.2 1,376,070 - Pf1N1B4_1214 0.36 -1.8 1,376,070 - Pf1N1B4_1214 0.36 +1.5 1,376,101 + Pf1N1B4_1214 0.42 -1.2 1,376,293 + Pf1N1B4_1214 0.78 -1.2 1,376,293 + Pf1N1B4_1214 0.78 +0.2 1,376,293 + Pf1N1B4_1214 0.78 +0.2 1,376,364 + +0.9 1,376,365 - +1.4
Or see this region's nucleotide sequence