Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_107

Experiment: carbon source Sucrose 5mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_104 and Pf1N1B4_105 overlap by 4 nucleotidesPf1N1B4_105 and Pf1N1B4_106 are separated by 3 nucleotidesPf1N1B4_106 and Pf1N1B4_107 are separated by 38 nucleotidesPf1N1B4_107 and Pf1N1B4_108 are separated by 243 nucleotides Pf1N1B4_104 - Sensor protein PhoQ (EC 2.7.13.3), at 110,365 to 111,678 _104 Pf1N1B4_105 - Two-component system response regulator QseB, at 111,675 to 112,343 _105 Pf1N1B4_106 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 112,347 to 112,628 _106 Pf1N1B4_107 - FIG00857858: hypothetical protein, at 112,667 to 112,975 _107 Pf1N1B4_108 - FIG00954456: hypothetical protein, at 113,219 to 114,298 _108 Position (kb) 112 113Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 111.996 kb on + strand, within Pf1N1B4_105at 112.232 kb on - strand, within Pf1N1B4_105at 112.364 kb on + strandat 112.364 kb on + strandat 112.368 kb on + strandat 112.368 kb on + strandat 112.368 kb on + strandat 112.369 kb on - strandat 112.395 kb on + strand, within Pf1N1B4_106at 112.409 kb on + strand, within Pf1N1B4_106at 112.438 kb on + strand, within Pf1N1B4_106at 112.438 kb on + strandat 112.438 kb on + strand, within Pf1N1B4_106at 112.444 kb on + strand, within Pf1N1B4_106at 112.668 kb on + strandat 112.668 kb on + strandat 112.668 kb on + strandat 112.668 kb on + strandat 112.668 kb on + strandat 112.761 kb on + strand, within Pf1N1B4_107at 112.762 kb on - strand, within Pf1N1B4_107at 112.767 kb on + strand, within Pf1N1B4_107at 112.767 kb on + strand, within Pf1N1B4_107at 112.767 kb on + strand, within Pf1N1B4_107at 112.768 kb on - strand, within Pf1N1B4_107at 112.768 kb on - strand, within Pf1N1B4_107at 112.768 kb on - strand, within Pf1N1B4_107at 112.768 kb on - strand, within Pf1N1B4_107at 112.777 kb on - strandat 112.864 kb on + strand, within Pf1N1B4_107at 112.865 kb on - strand, within Pf1N1B4_107at 112.988 kb on + strandat 113.006 kb on + strandat 113.006 kb on + strandat 113.007 kb on - strandat 113.007 kb on - strandat 113.016 kb on - strandat 113.016 kb on - strandat 113.016 kb on - strandat 113.020 kb on + strandat 113.020 kb on + strandat 113.035 kb on - strandat 113.087 kb on - strandat 113.126 kb on + strandat 113.127 kb on - strandat 113.128 kb on + strandat 113.154 kb on + strandat 113.154 kb on + strandat 113.155 kb on - strandat 113.510 kb on + strand, within Pf1N1B4_108at 113.510 kb on + strand, within Pf1N1B4_108at 113.961 kb on - strand, within Pf1N1B4_108

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Per-strain Table

Position Strand Gene LocusTag Fraction carbon source Sucrose 5mM
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111,996 + Pf1N1B4_105 0.48 -2.9
112,232 - Pf1N1B4_105 0.83 -2.0
112,364 + +1.1
112,364 + +0.8
112,368 + +2.1
112,368 + +2.3
112,368 + -2.6
112,369 - -2.4
112,395 + Pf1N1B4_106 0.17 -0.1
112,409 + Pf1N1B4_106 0.22 +0.6
112,438 + Pf1N1B4_106 0.32 +0.1
112,438 + -0.4
112,438 + Pf1N1B4_106 0.32 -0.1
112,444 + Pf1N1B4_106 0.34 +0.4
112,668 + +0.4
112,668 + +1.3
112,668 + -2.0
112,668 + +0.0
112,668 + -0.3
112,761 + Pf1N1B4_107 0.30 +2.1
112,762 - Pf1N1B4_107 0.31 -0.9
112,767 + Pf1N1B4_107 0.32 +0.6
112,767 + Pf1N1B4_107 0.32 +0.8
112,767 + Pf1N1B4_107 0.32 -2.5
112,768 - Pf1N1B4_107 0.33 +0.5
112,768 - Pf1N1B4_107 0.33 -1.2
112,768 - Pf1N1B4_107 0.33 +0.9
112,768 - Pf1N1B4_107 0.33 +1.1
112,777 - -0.2
112,864 + Pf1N1B4_107 0.64 -0.5
112,865 - Pf1N1B4_107 0.64 +1.7
112,988 + -1.0
113,006 + -1.0
113,006 + -1.3
113,007 - +2.1
113,007 - +0.1
113,016 - +1.6
113,016 - +0.8
113,016 - +0.2
113,020 + -0.3
113,020 + -2.4
113,035 - +2.1
113,087 - +3.7
113,126 + -1.0
113,127 - +0.3
113,128 + -2.8
113,154 + -1.3
113,154 + -0.2
113,155 - -1.6
113,510 + Pf1N1B4_108 0.27 +0.8
113,510 + Pf1N1B4_108 0.27 +2.4
113,961 - Pf1N1B4_108 0.69 -1.0

Or see this region's nucleotide sequence