Experiment: MoLS4 with S-adenosyl Homocysteine 50uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2049 and DVU2051 are separated by 791 nucleotides DVU2051 and tRNA-Met are separated by 220 nucleotides tRNA-Met and dmt are separated by 102 nucleotides
DVU2049: DVU2049 - transposase OrfA, IS3 family, degenerate (TIGR), at 2,126,385 to 2,126,851
DVU2049
DVU2051: DVU2051 - hypothetical protein (TIGR), at 2,127,643 to 2,128,941
DVU2051
DVU_tRNA-Met-4: tRNA-Met - tRNA with anticodon CAT for Met (VIMSS-AUTO), at 2,129,162 to 2,129,238
tRNA-Met
DVU2052: dmt - glycosyl transferase, group 2 family protein (TIGR), at 2,129,341 to 2,130,063
dmt
Position (kb)
2127
2128
2129 Strain fitness (log2 ratio)
-1
0
1 at 2126.645 kb on + strand, within DVU2049 at 2126.683 kb on - strand, within DVU2049 at 2126.691 kb on + strand, within DVU2049 at 2126.698 kb on + strand, within DVU2049 at 2126.922 kb on - strand at 2126.965 kb on + strand at 2126.973 kb on - strand at 2127.121 kb on + strand at 2127.140 kb on + strand at 2127.140 kb on + strand at 2127.148 kb on - strand at 2127.150 kb on + strand at 2127.152 kb on + strand at 2127.199 kb on + strand at 2127.200 kb on + strand at 2127.200 kb on + strand at 2127.208 kb on - strand at 2127.208 kb on - strand at 2127.221 kb on + strand at 2127.410 kb on - strand at 2127.555 kb on - strand at 2128.493 kb on + strand, within DVU2051 at 2128.500 kb on + strand, within DVU2051 at 2128.739 kb on + strand, within DVU2051 at 2128.824 kb on - strand at 2129.077 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 with S-adenosyl Homocysteine 50uM remove 2,126,645 + DVU2049 0.56 -0.1 2,126,683 - DVU2049 0.64 -0.1 2,126,691 + DVU2049 0.66 -0.1 2,126,698 + DVU2049 0.67 -1.3 2,126,922 - -0.3 2,126,965 + -1.1 2,126,973 - -0.2 2,127,121 + -0.8 2,127,140 + -0.7 2,127,140 + -0.4 2,127,148 - +0.1 2,127,150 + +0.5 2,127,152 + -1.0 2,127,199 + -0.7 2,127,200 + -1.0 2,127,200 + +0.1 2,127,208 - -0.7 2,127,208 - +0.1 2,127,221 + +1.2 2,127,410 - -0.0 2,127,555 - +0.2 2,128,493 + DVU2051 0.65 -0.9 2,128,500 + DVU2051 0.66 -1.2 2,128,739 + DVU2051 0.84 -0.4 2,128,824 - -0.0 2,129,077 + -0.4
Or see this region's nucleotide sequence