Experiment: KB with Cyclopamine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4860 and Psyr_4861 are separated by 711 nucleotides Psyr_4861 and Psyr_4862 overlap by 4 nucleotides Psyr_4862 and Psyr_4863 overlap by 4 nucleotides
Psyr_4860: Psyr_4860 - Integrase, catalytic region, at 5,767,943 to 5,768,749
_4860
Psyr_4861: Psyr_4861 - Binding-protein-dependent transport systems inner membrane component, at 5,769,461 to 5,770,342
_4861
Psyr_4862: Psyr_4862 - Binding-protein-dependent transport systems inner membrane component, at 5,770,339 to 5,771,259
_4862
Psyr_4863: Psyr_4863 - Spermidine/putrescine ABC transporter ATP-binding subunit, at 5,771,256 to 5,772,398
_4863
Position (kb) 5769
5770
5771 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5768.498 kb on + strand, within Psyr_4860 at 5769.024 kb on + strand at 5769.268 kb on + strand at 5769.268 kb on + strand at 5769.268 kb on + strand at 5769.268 kb on + strand at 5769.297 kb on + strand at 5769.297 kb on + strand at 5769.791 kb on - strand, within Psyr_4861 at 5769.870 kb on - strand, within Psyr_4861 at 5769.870 kb on - strand, within Psyr_4861 at 5769.870 kb on - strand, within Psyr_4861 at 5769.897 kb on + strand, within Psyr_4861 at 5769.897 kb on + strand, within Psyr_4861 at 5769.897 kb on + strand, within Psyr_4861 at 5769.897 kb on + strand, within Psyr_4861 at 5769.897 kb on + strand, within Psyr_4861 at 5769.898 kb on - strand, within Psyr_4861 at 5769.898 kb on - strand, within Psyr_4861 at 5770.026 kb on - strand, within Psyr_4861 at 5770.137 kb on + strand, within Psyr_4861 at 5770.138 kb on - strand, within Psyr_4861 at 5770.203 kb on + strand, within Psyr_4861 at 5770.203 kb on + strand, within Psyr_4861 at 5770.203 kb on + strand, within Psyr_4861 at 5770.204 kb on - strand, within Psyr_4861 at 5770.204 kb on - strand, within Psyr_4861 at 5770.260 kb on + strand at 5770.260 kb on + strand at 5770.260 kb on + strand at 5770.260 kb on + strand at 5770.261 kb on - strand at 5770.261 kb on - strand at 5770.261 kb on - strand at 5770.261 kb on - strand at 5770.287 kb on + strand at 5770.287 kb on + strand at 5770.287 kb on + strand at 5770.287 kb on + strand at 5770.287 kb on + strand at 5770.287 kb on + strand at 5770.287 kb on + strand at 5770.287 kb on + strand at 5770.288 kb on - strand at 5770.288 kb on - strand at 5770.288 kb on - strand at 5770.288 kb on - strand at 5770.341 kb on + strand at 5770.549 kb on + strand, within Psyr_4862 at 5770.683 kb on - strand, within Psyr_4862 at 5770.710 kb on - strand, within Psyr_4862 at 5770.710 kb on - strand, within Psyr_4862 at 5770.715 kb on + strand, within Psyr_4862 at 5770.715 kb on + strand, within Psyr_4862 at 5770.715 kb on + strand, within Psyr_4862 at 5770.716 kb on - strand, within Psyr_4862 at 5770.716 kb on - strand, within Psyr_4862 at 5770.730 kb on + strand, within Psyr_4862 at 5770.731 kb on - strand, within Psyr_4862 at 5770.731 kb on - strand, within Psyr_4862 at 5770.731 kb on - strand, within Psyr_4862 at 5770.731 kb on - strand, within Psyr_4862 at 5770.839 kb on - strand, within Psyr_4862 at 5771.009 kb on + strand, within Psyr_4862 at 5771.009 kb on + strand, within Psyr_4862 at 5771.010 kb on - strand, within Psyr_4862 at 5771.036 kb on + strand, within Psyr_4862 at 5771.036 kb on + strand, within Psyr_4862 at 5771.036 kb on + strand, within Psyr_4862 at 5771.036 kb on + strand, within Psyr_4862 at 5771.036 kb on + strand, within Psyr_4862 at 5771.036 kb on + strand, within Psyr_4862 at 5771.036 kb on + strand, within Psyr_4862 at 5771.037 kb on - strand, within Psyr_4862 at 5771.037 kb on - strand, within Psyr_4862 at 5771.037 kb on - strand, within Psyr_4862 at 5771.037 kb on - strand, within Psyr_4862 at 5771.037 kb on - strand, within Psyr_4862 at 5771.037 kb on - strand, within Psyr_4862 at 5771.037 kb on - strand, within Psyr_4862 at 5771.079 kb on - strand, within Psyr_4862 at 5771.079 kb on - strand, within Psyr_4862 at 5771.096 kb on + strand, within Psyr_4862 at 5771.096 kb on + strand, within Psyr_4862 at 5771.268 kb on + strand at 5771.299 kb on + strand at 5771.299 kb on + strand at 5771.299 kb on + strand at 5771.299 kb on + strand at 5771.299 kb on + strand at 5771.300 kb on - strand at 5771.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Cyclopamine 10 mM remove 5,768,498 + Psyr_4860 0.69 +0.0 5,769,024 + +0.2 5,769,268 + +0.6 5,769,268 + +1.1 5,769,268 + +0.5 5,769,268 + +0.3 5,769,297 + +0.0 5,769,297 + +0.4 5,769,791 - Psyr_4861 0.37 -1.0 5,769,870 - Psyr_4861 0.46 -0.5 5,769,870 - Psyr_4861 0.46 +0.2 5,769,870 - Psyr_4861 0.46 +0.5 5,769,897 + Psyr_4861 0.49 -0.1 5,769,897 + Psyr_4861 0.49 +1.4 5,769,897 + Psyr_4861 0.49 -0.4 5,769,897 + Psyr_4861 0.49 +0.4 5,769,897 + Psyr_4861 0.49 -0.6 5,769,898 - Psyr_4861 0.50 +0.2 5,769,898 - Psyr_4861 0.50 +0.1 5,770,026 - Psyr_4861 0.64 +1.5 5,770,137 + Psyr_4861 0.77 +0.7 5,770,138 - Psyr_4861 0.77 +0.5 5,770,203 + Psyr_4861 0.84 +0.4 5,770,203 + Psyr_4861 0.84 -0.1 5,770,203 + Psyr_4861 0.84 +0.9 5,770,204 - Psyr_4861 0.84 +0.4 5,770,204 - Psyr_4861 0.84 +1.0 5,770,260 + -0.5 5,770,260 + +1.4 5,770,260 + +0.2 5,770,260 + +0.3 5,770,261 - +2.8 5,770,261 - +0.7 5,770,261 - -0.1 5,770,261 - -1.6 5,770,287 + -0.5 5,770,287 + +0.2 5,770,287 + +1.4 5,770,287 + -0.4 5,770,287 + -1.5 5,770,287 + -1.1 5,770,287 + -0.2 5,770,287 + +1.2 5,770,288 - -0.2 5,770,288 - -0.5 5,770,288 - -0.1 5,770,288 - -0.5 5,770,341 + -0.0 5,770,549 + Psyr_4862 0.23 -0.4 5,770,683 - Psyr_4862 0.37 +0.7 5,770,710 - Psyr_4862 0.40 -0.1 5,770,710 - Psyr_4862 0.40 -0.5 5,770,715 + Psyr_4862 0.41 -0.3 5,770,715 + Psyr_4862 0.41 +1.4 5,770,715 + Psyr_4862 0.41 -0.8 5,770,716 - Psyr_4862 0.41 -0.4 5,770,716 - Psyr_4862 0.41 -0.1 5,770,730 + Psyr_4862 0.42 -1.0 5,770,731 - Psyr_4862 0.43 -0.4 5,770,731 - Psyr_4862 0.43 +0.4 5,770,731 - Psyr_4862 0.43 +0.5 5,770,731 - Psyr_4862 0.43 +0.7 5,770,839 - Psyr_4862 0.54 +0.3 5,771,009 + Psyr_4862 0.73 +0.2 5,771,009 + Psyr_4862 0.73 -0.6 5,771,010 - Psyr_4862 0.73 -2.6 5,771,036 + Psyr_4862 0.76 +0.5 5,771,036 + Psyr_4862 0.76 -0.3 5,771,036 + Psyr_4862 0.76 -0.0 5,771,036 + Psyr_4862 0.76 -1.5 5,771,036 + Psyr_4862 0.76 -1.0 5,771,036 + Psyr_4862 0.76 -0.9 5,771,036 + Psyr_4862 0.76 -0.4 5,771,037 - Psyr_4862 0.76 +1.6 5,771,037 - Psyr_4862 0.76 -1.0 5,771,037 - Psyr_4862 0.76 +0.4 5,771,037 - Psyr_4862 0.76 -0.3 5,771,037 - Psyr_4862 0.76 -0.9 5,771,037 - Psyr_4862 0.76 +0.9 5,771,037 - Psyr_4862 0.76 -0.1 5,771,079 - Psyr_4862 0.80 -0.5 5,771,079 - Psyr_4862 0.80 +1.2 5,771,096 + Psyr_4862 0.82 -1.0 5,771,096 + Psyr_4862 0.82 -0.5 5,771,268 + +0.4 5,771,299 + -0.4 5,771,299 + +0.2 5,771,299 + -0.6 5,771,299 + +1.2 5,771,299 + -2.0 5,771,300 - -1.2 5,771,300 - -1.4
Or see this region's nucleotide sequence