Experiment: KB with Cyclopamine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3481 and Psyr_3482 are separated by 278 nucleotides Psyr_3482 and Psyr_3483 are separated by 10 nucleotides Psyr_3483 and Psyr_3484 are separated by 73 nucleotides
Psyr_3481: Psyr_3481 - Flagellar basal-body rod protein FlgB, at 4,152,137 to 4,152,544
_3481
Psyr_3482: Psyr_3482 - Protein of unknown function DUF1348, at 4,152,823 to 4,153,296
_3482
Psyr_3483: Psyr_3483 - Cyanase, at 4,153,307 to 4,153,774
_3483
Psyr_3484: Psyr_3484 - regulatory protein, LysR:LysR, substrate-binding protein, at 4,153,848 to 4,154,762
_3484
Position (kb)
4153
4154 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4152.332 kb on + strand, within Psyr_3481 at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.512 kb on + strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.513 kb on - strand at 4152.884 kb on + strand, within Psyr_3482 at 4152.884 kb on + strand, within Psyr_3482 at 4152.885 kb on - strand, within Psyr_3482 at 4152.885 kb on - strand, within Psyr_3482 at 4152.980 kb on + strand, within Psyr_3482 at 4153.025 kb on + strand, within Psyr_3482 at 4153.093 kb on - strand, within Psyr_3482 at 4153.163 kb on + strand, within Psyr_3482 at 4153.163 kb on + strand, within Psyr_3482 at 4153.163 kb on + strand, within Psyr_3482 at 4153.163 kb on + strand, within Psyr_3482 at 4153.163 kb on + strand, within Psyr_3482 at 4153.178 kb on + strand, within Psyr_3482 at 4153.178 kb on + strand, within Psyr_3482 at 4153.178 kb on + strand, within Psyr_3482 at 4153.468 kb on - strand, within Psyr_3483 at 4153.495 kb on - strand, within Psyr_3483 at 4153.495 kb on - strand, within Psyr_3483 at 4153.650 kb on + strand, within Psyr_3483 at 4153.650 kb on + strand, within Psyr_3483 at 4153.650 kb on + strand, within Psyr_3483 at 4153.650 kb on + strand, within Psyr_3483 at 4153.650 kb on + strand, within Psyr_3483 at 4153.650 kb on + strand, within Psyr_3483 at 4153.650 kb on + strand, within Psyr_3483 at 4153.650 kb on + strand, within Psyr_3483 at 4153.650 kb on + strand, within Psyr_3483 at 4153.650 kb on + strand, within Psyr_3483 at 4153.650 kb on + strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.651 kb on - strand, within Psyr_3483 at 4153.759 kb on + strand at 4153.759 kb on + strand at 4153.759 kb on + strand at 4153.760 kb on - strand at 4153.760 kb on - strand at 4153.760 kb on - strand at 4153.760 kb on - strand at 4153.775 kb on - strand at 4153.775 kb on - strand at 4153.775 kb on - strand at 4153.775 kb on - strand at 4153.779 kb on - strand at 4153.779 kb on - strand at 4153.822 kb on - strand at 4153.829 kb on + strand at 4153.847 kb on + strand at 4153.873 kb on - strand at 4153.899 kb on - strand at 4153.899 kb on - strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.900 kb on + strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.901 kb on - strand at 4153.905 kb on + strand at 4153.905 kb on + strand at 4153.905 kb on + strand at 4154.073 kb on + strand, within Psyr_3484 at 4154.073 kb on + strand, within Psyr_3484 at 4154.074 kb on - strand, within Psyr_3484 at 4154.074 kb on - strand, within Psyr_3484 at 4154.180 kb on + strand, within Psyr_3484 at 4154.194 kb on - strand, within Psyr_3484 at 4154.347 kb on + strand, within Psyr_3484 at 4154.347 kb on + strand, within Psyr_3484 at 4154.691 kb on - strand at 4154.758 kb on + strand at 4154.758 kb on + strand at 4154.758 kb on + strand at 4154.759 kb on - strand at 4154.759 kb on - strand at 4154.759 kb on - strand at 4154.759 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Cyclopamine 10 mM remove 4,152,332 + Psyr_3481 0.48 -0.6 4,152,512 + -1.3 4,152,512 + +0.2 4,152,512 + +0.7 4,152,512 + +0.0 4,152,512 + -2.0 4,152,512 + +0.5 4,152,512 + +0.9 4,152,512 + +0.4 4,152,512 + +1.2 4,152,512 + -0.1 4,152,512 + -0.8 4,152,512 + +0.1 4,152,512 + +0.7 4,152,512 + +0.2 4,152,512 + +0.1 4,152,512 + -0.7 4,152,512 + +0.2 4,152,513 - +0.7 4,152,513 - -0.4 4,152,513 - -0.4 4,152,513 - +0.2 4,152,513 - +0.2 4,152,513 - -1.1 4,152,513 - -0.5 4,152,513 - +0.6 4,152,513 - +0.5 4,152,513 - -0.6 4,152,513 - +0.7 4,152,513 - +0.5 4,152,513 - +0.2 4,152,513 - +0.0 4,152,513 - +0.9 4,152,884 + Psyr_3482 0.13 +0.1 4,152,884 + Psyr_3482 0.13 +0.5 4,152,885 - Psyr_3482 0.13 -0.5 4,152,885 - Psyr_3482 0.13 +1.4 4,152,980 + Psyr_3482 0.33 +0.2 4,153,025 + Psyr_3482 0.43 +1.1 4,153,093 - Psyr_3482 0.57 +0.5 4,153,163 + Psyr_3482 0.72 -0.1 4,153,163 + Psyr_3482 0.72 +0.0 4,153,163 + Psyr_3482 0.72 -0.3 4,153,163 + Psyr_3482 0.72 +0.2 4,153,163 + Psyr_3482 0.72 +0.5 4,153,178 + Psyr_3482 0.75 -1.1 4,153,178 + Psyr_3482 0.75 +0.8 4,153,178 + Psyr_3482 0.75 +0.6 4,153,468 - Psyr_3483 0.34 -0.9 4,153,495 - Psyr_3483 0.40 +0.0 4,153,495 - Psyr_3483 0.40 -0.1 4,153,650 + Psyr_3483 0.73 -0.4 4,153,650 + Psyr_3483 0.73 -0.1 4,153,650 + Psyr_3483 0.73 +0.3 4,153,650 + Psyr_3483 0.73 -0.0 4,153,650 + Psyr_3483 0.73 +0.2 4,153,650 + Psyr_3483 0.73 +1.9 4,153,650 + Psyr_3483 0.73 +0.0 4,153,650 + Psyr_3483 0.73 -0.8 4,153,650 + Psyr_3483 0.73 +1.9 4,153,650 + Psyr_3483 0.73 +0.2 4,153,650 + Psyr_3483 0.73 +0.7 4,153,651 - Psyr_3483 0.74 +1.2 4,153,651 - Psyr_3483 0.74 +0.2 4,153,651 - Psyr_3483 0.74 +1.3 4,153,651 - Psyr_3483 0.74 +0.4 4,153,651 - Psyr_3483 0.74 +0.9 4,153,651 - Psyr_3483 0.74 +1.3 4,153,651 - Psyr_3483 0.74 -0.5 4,153,651 - Psyr_3483 0.74 +0.3 4,153,651 - Psyr_3483 0.74 +1.0 4,153,651 - Psyr_3483 0.74 +0.8 4,153,651 - Psyr_3483 0.74 +0.3 4,153,651 - Psyr_3483 0.74 +1.2 4,153,651 - Psyr_3483 0.74 -0.5 4,153,651 - Psyr_3483 0.74 -0.3 4,153,759 + +0.5 4,153,759 + -0.3 4,153,759 + +1.0 4,153,760 - +0.9 4,153,760 - -0.7 4,153,760 - +0.1 4,153,760 - +1.5 4,153,775 - +1.1 4,153,775 - +0.1 4,153,775 - +0.2 4,153,775 - -1.1 4,153,779 - +0.7 4,153,779 - -0.7 4,153,822 - -0.3 4,153,829 + -0.2 4,153,847 + +1.2 4,153,873 - -0.5 4,153,899 - +0.2 4,153,899 - +0.7 4,153,900 + -0.2 4,153,900 + -1.4 4,153,900 + -0.5 4,153,900 + +1.4 4,153,900 + +0.5 4,153,900 + -0.5 4,153,900 + -0.9 4,153,900 + -2.5 4,153,900 + -0.6 4,153,900 + +0.4 4,153,900 + -0.7 4,153,900 + +1.8 4,153,900 + +0.1 4,153,900 + +1.1 4,153,900 + +0.7 4,153,900 + +0.6 4,153,900 + -0.7 4,153,900 + +0.3 4,153,900 + -1.5 4,153,900 + +2.5 4,153,900 + -0.6 4,153,900 + -0.4 4,153,900 + -0.4 4,153,901 - -0.5 4,153,901 - -0.2 4,153,901 - +1.1 4,153,901 - -0.9 4,153,901 - -0.7 4,153,901 - +0.5 4,153,901 - -0.0 4,153,901 - -1.5 4,153,901 - +0.2 4,153,901 - +1.0 4,153,901 - +0.4 4,153,901 - +1.0 4,153,901 - +0.1 4,153,901 - +0.5 4,153,901 - -1.1 4,153,901 - -0.5 4,153,901 - +1.0 4,153,901 - -0.3 4,153,901 - +0.6 4,153,901 - -0.0 4,153,901 - +0.1 4,153,905 + +0.9 4,153,905 + +0.6 4,153,905 + +0.4 4,154,073 + Psyr_3484 0.25 +0.6 4,154,073 + Psyr_3484 0.25 +0.3 4,154,074 - Psyr_3484 0.25 +1.0 4,154,074 - Psyr_3484 0.25 +0.3 4,154,180 + Psyr_3484 0.36 +0.6 4,154,194 - Psyr_3484 0.38 -0.0 4,154,347 + Psyr_3484 0.55 -0.7 4,154,347 + Psyr_3484 0.55 +0.5 4,154,691 - -0.4 4,154,758 + +0.5 4,154,758 + +1.5 4,154,758 + -3.7 4,154,759 - -1.9 4,154,759 - +0.4 4,154,759 - -0.7 4,154,759 - +2.6
Or see this region's nucleotide sequence