Experiment: KB with Cyclopamine 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2567 and Psyr_2568 are separated by 29 nucleotides Psyr_2568 and Psyr_2569 are separated by 208 nucleotides Psyr_2569 and Psyr_2570 are separated by 43 nucleotides
Psyr_2567: Psyr_2567 - Zinc-containing alcohol dehydrogenase superfamily, at 2,980,696 to 2,981,811
_2567
Psyr_2568: Psyr_2568 - Short-chain dehydrogenase/reductase SDR, at 2,981,841 to 2,982,596
_2568
Psyr_2569: Psyr_2569 - monosaccharide ABC transporter substrate-binding protein, CUT2 family, at 2,982,805 to 2,983,815
_2569
Psyr_2570: Psyr_2570 - monosaccharide ABC transporter membrane protein, CUT2 family, at 2,983,859 to 2,984,836
_2570
Position (kb)
2982
2983
2984 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2981.871 kb on + strand at 2981.932 kb on - strand, within Psyr_2568 at 2982.643 kb on - strand at 2982.727 kb on + strand at 2982.727 kb on + strand at 2982.830 kb on + strand at 2982.831 kb on - strand at 2982.878 kb on + strand at 2982.950 kb on + strand, within Psyr_2569 at 2982.950 kb on + strand, within Psyr_2569 at 2982.951 kb on - strand, within Psyr_2569 at 2983.433 kb on + strand, within Psyr_2569 at 2983.434 kb on - strand, within Psyr_2569 at 2983.557 kb on - strand, within Psyr_2569 at 2983.579 kb on + strand, within Psyr_2569 at 2983.607 kb on + strand, within Psyr_2569 at 2983.607 kb on + strand, within Psyr_2569 at 2983.607 kb on + strand, within Psyr_2569 at 2983.607 kb on + strand, within Psyr_2569 at 2983.608 kb on - strand, within Psyr_2569 at 2983.608 kb on - strand, within Psyr_2569 at 2983.608 kb on - strand, within Psyr_2569 at 2983.608 kb on - strand, within Psyr_2569 at 2983.730 kb on + strand at 2983.730 kb on + strand at 2983.759 kb on - strand at 2983.838 kb on + strand at 2983.839 kb on - strand at 2983.932 kb on + strand at 2983.933 kb on - strand at 2983.936 kb on - strand at 2984.076 kb on + strand, within Psyr_2570 at 2984.163 kb on + strand, within Psyr_2570 at 2984.236 kb on - strand, within Psyr_2570 at 2984.251 kb on - strand, within Psyr_2570 at 2984.259 kb on + strand, within Psyr_2570 at 2984.303 kb on - strand, within Psyr_2570 at 2984.352 kb on + strand, within Psyr_2570 at 2984.483 kb on + strand, within Psyr_2570 at 2984.628 kb on + strand, within Psyr_2570 at 2984.628 kb on + strand, within Psyr_2570 at 2984.628 kb on + strand, within Psyr_2570 at 2984.628 kb on + strand, within Psyr_2570 at 2984.629 kb on - strand, within Psyr_2570 at 2984.629 kb on - strand, within Psyr_2570 at 2984.629 kb on - strand, within Psyr_2570 at 2984.743 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Cyclopamine 10 mM remove 2,981,871 + +0.7 2,981,932 - Psyr_2568 0.12 +0.5 2,982,643 - +0.5 2,982,727 + +1.3 2,982,727 + +0.1 2,982,830 + +0.1 2,982,831 - -0.1 2,982,878 + +0.8 2,982,950 + Psyr_2569 0.14 -1.3 2,982,950 + Psyr_2569 0.14 -1.1 2,982,951 - Psyr_2569 0.14 -0.1 2,983,433 + Psyr_2569 0.62 -0.9 2,983,434 - Psyr_2569 0.62 -0.7 2,983,557 - Psyr_2569 0.74 +0.1 2,983,579 + Psyr_2569 0.77 -0.6 2,983,607 + Psyr_2569 0.79 -0.8 2,983,607 + Psyr_2569 0.79 -1.8 2,983,607 + Psyr_2569 0.79 -0.2 2,983,607 + Psyr_2569 0.79 -0.2 2,983,608 - Psyr_2569 0.79 -0.5 2,983,608 - Psyr_2569 0.79 -0.7 2,983,608 - Psyr_2569 0.79 -0.5 2,983,608 - Psyr_2569 0.79 -0.1 2,983,730 + +0.3 2,983,730 + -0.6 2,983,759 - -0.2 2,983,838 + -0.5 2,983,839 - -1.6 2,983,932 + +0.4 2,983,933 - +0.1 2,983,936 - -0.4 2,984,076 + Psyr_2570 0.22 -0.1 2,984,163 + Psyr_2570 0.31 +0.6 2,984,236 - Psyr_2570 0.39 +0.8 2,984,251 - Psyr_2570 0.40 +0.4 2,984,259 + Psyr_2570 0.41 +1.9 2,984,303 - Psyr_2570 0.45 +0.0 2,984,352 + Psyr_2570 0.50 +0.2 2,984,483 + Psyr_2570 0.64 -0.9 2,984,628 + Psyr_2570 0.79 -1.1 2,984,628 + Psyr_2570 0.79 -0.0 2,984,628 + Psyr_2570 0.79 +2.7 2,984,628 + Psyr_2570 0.79 +0.2 2,984,629 - Psyr_2570 0.79 +0.5 2,984,629 - Psyr_2570 0.79 -0.7 2,984,629 - Psyr_2570 0.79 -0.1 2,984,743 + +0.7
Or see this region's nucleotide sequence