Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0257

Experiment: KB with Cyclopamine 10 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0255 and Psyr_0256 are separated by 226 nucleotidesPsyr_0256 and Psyr_0257 overlap by 1 nucleotidesPsyr_0257 and Psyr_0258 are separated by 299 nucleotides Psyr_0255: Psyr_0255 - glutamate-cysteine ligase, at 269,298 to 270,905 _0255 Psyr_0256: Psyr_0256 - Phenylacetic acid degradation-related protein, at 271,132 to 271,515 _0256 Psyr_0257: Psyr_0257 - RNA binding S1, at 271,515 to 273,833 _0257 Psyr_0258: Psyr_0258 - Two-component response regulator OmpR, at 274,133 to 274,873 _0258 Position (kb) 271 272 273 274Strain fitness (log2 ratio) -2 -1 0 1 2 3at 270.896 kb on - strandat 270.896 kb on - strandat 270.944 kb on + strandat 270.944 kb on + strandat 270.971 kb on - strandat 271.022 kb on - strandat 271.065 kb on - strandat 271.088 kb on + strandat 271.133 kb on + strandat 271.133 kb on + strandat 271.134 kb on - strandat 271.134 kb on - strandat 271.134 kb on - strandat 271.274 kb on + strand, within Psyr_0256at 271.274 kb on + strand, within Psyr_0256at 271.274 kb on + strand, within Psyr_0256at 271.274 kb on + strand, within Psyr_0256at 271.275 kb on - strand, within Psyr_0256at 271.275 kb on - strand, within Psyr_0256at 271.275 kb on - strand, within Psyr_0256at 271.275 kb on - strand, within Psyr_0256at 271.275 kb on - strand, within Psyr_0256at 271.275 kb on - strand, within Psyr_0256at 271.275 kb on - strand, within Psyr_0256at 271.275 kb on - strand, within Psyr_0256at 271.275 kb on - strand, within Psyr_0256at 271.275 kb on - strand, within Psyr_0256at 272.432 kb on - strand, within Psyr_0257at 272.774 kb on - strand, within Psyr_0257at 272.815 kb on - strand, within Psyr_0257at 272.983 kb on + strand, within Psyr_0257at 272.984 kb on - strand, within Psyr_0257at 272.984 kb on - strand, within Psyr_0257at 272.984 kb on - strand, within Psyr_0257at 273.349 kb on + strand, within Psyr_0257at 273.349 kb on + strand, within Psyr_0257at 273.349 kb on + strand, within Psyr_0257at 273.349 kb on + strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.350 kb on - strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.375 kb on + strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.376 kb on - strand, within Psyr_0257at 273.378 kb on - strand, within Psyr_0257at 273.518 kb on + strand, within Psyr_0257at 273.518 kb on + strand, within Psyr_0257at 273.518 kb on + strand, within Psyr_0257at 273.518 kb on + strand, within Psyr_0257at 273.518 kb on + strand, within Psyr_0257at 273.518 kb on + strand, within Psyr_0257at 273.519 kb on - strand, within Psyr_0257at 273.519 kb on - strand, within Psyr_0257at 273.519 kb on - strand, within Psyr_0257at 273.519 kb on - strand, within Psyr_0257at 273.519 kb on - strand, within Psyr_0257at 273.519 kb on - strand, within Psyr_0257at 273.521 kb on - strand, within Psyr_0257at 273.521 kb on - strand, within Psyr_0257at 273.521 kb on - strand, within Psyr_0257at 273.521 kb on - strand, within Psyr_0257at 273.529 kb on + strand, within Psyr_0257at 273.529 kb on + strand, within Psyr_0257at 273.529 kb on + strand, within Psyr_0257at 273.529 kb on + strand, within Psyr_0257at 273.529 kb on + strand, within Psyr_0257at 273.530 kb on - strand, within Psyr_0257at 273.530 kb on - strand, within Psyr_0257at 273.530 kb on - strand, within Psyr_0257at 273.530 kb on - strand, within Psyr_0257at 273.656 kb on - strandat 273.656 kb on - strandat 273.760 kb on - strandat 273.793 kb on - strandat 273.834 kb on - strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.868 kb on + strandat 273.869 kb on - strandat 273.869 kb on - strandat 273.869 kb on - strandat 273.869 kb on - strandat 273.869 kb on - strandat 273.872 kb on + strandat 273.872 kb on + strandat 273.872 kb on + strandat 273.872 kb on + strandat 273.873 kb on - strandat 273.873 kb on - strandat 273.909 kb on + strandat 273.970 kb on + strandat 273.970 kb on + strandat 273.971 kb on - strandat 273.973 kb on - strandat 273.990 kb on + strandat 274.096 kb on + strandat 274.096 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Cyclopamine 10 mM
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270,896 - -1.8
270,896 - -0.2
270,944 + +0.8
270,944 + +0.3
270,971 - -0.1
271,022 - -0.3
271,065 - -0.1
271,088 + +0.8
271,133 + +0.4
271,133 + -0.2
271,134 - +1.1
271,134 - +0.3
271,134 - -0.0
271,274 + Psyr_0256 0.37 +0.5
271,274 + Psyr_0256 0.37 -0.4
271,274 + Psyr_0256 0.37 +0.2
271,274 + Psyr_0256 0.37 +0.4
271,275 - Psyr_0256 0.37 -0.1
271,275 - Psyr_0256 0.37 -0.1
271,275 - Psyr_0256 0.37 -0.1
271,275 - Psyr_0256 0.37 -0.3
271,275 - Psyr_0256 0.37 -0.3
271,275 - Psyr_0256 0.37 -0.5
271,275 - Psyr_0256 0.37 +0.1
271,275 - Psyr_0256 0.37 -0.6
271,275 - Psyr_0256 0.37 +0.2
271,275 - Psyr_0256 0.37 -1.5
272,432 - Psyr_0257 0.40 +0.5
272,774 - Psyr_0257 0.54 +0.5
272,815 - Psyr_0257 0.56 +0.1
272,983 + Psyr_0257 0.63 +0.1
272,984 - Psyr_0257 0.63 +0.2
272,984 - Psyr_0257 0.63 +0.8
272,984 - Psyr_0257 0.63 +1.2
273,349 + Psyr_0257 0.79 -0.5
273,349 + Psyr_0257 0.79 -0.3
273,349 + Psyr_0257 0.79 -1.5
273,349 + Psyr_0257 0.79 -0.8
273,350 - Psyr_0257 0.79 -0.6
273,350 - Psyr_0257 0.79 -0.5
273,350 - Psyr_0257 0.79 +0.2
273,350 - Psyr_0257 0.79 +0.1
273,350 - Psyr_0257 0.79 -0.3
273,350 - Psyr_0257 0.79 +0.3
273,350 - Psyr_0257 0.79 +0.6
273,375 + Psyr_0257 0.80 -0.3
273,375 + Psyr_0257 0.80 -0.2
273,375 + Psyr_0257 0.80 +0.6
273,375 + Psyr_0257 0.80 -0.1
273,375 + Psyr_0257 0.80 -1.6
273,375 + Psyr_0257 0.80 +0.0
273,375 + Psyr_0257 0.80 +0.0
273,375 + Psyr_0257 0.80 +2.8
273,375 + Psyr_0257 0.80 -0.5
273,376 - Psyr_0257 0.80 -2.3
273,376 - Psyr_0257 0.80 +0.5
273,376 - Psyr_0257 0.80 -0.4
273,376 - Psyr_0257 0.80 +1.2
273,376 - Psyr_0257 0.80 -0.3
273,376 - Psyr_0257 0.80 -0.5
273,376 - Psyr_0257 0.80 -0.3
273,376 - Psyr_0257 0.80 +0.0
273,376 - Psyr_0257 0.80 +0.7
273,378 - Psyr_0257 0.80 +1.6
273,518 + Psyr_0257 0.86 +0.4
273,518 + Psyr_0257 0.86 -0.1
273,518 + Psyr_0257 0.86 +0.2
273,518 + Psyr_0257 0.86 +0.4
273,518 + Psyr_0257 0.86 -0.8
273,518 + Psyr_0257 0.86 +1.2
273,519 - Psyr_0257 0.86 +1.7
273,519 - Psyr_0257 0.86 -1.0
273,519 - Psyr_0257 0.86 -0.9
273,519 - Psyr_0257 0.86 -0.4
273,519 - Psyr_0257 0.86 +0.2
273,519 - Psyr_0257 0.86 +0.9
273,521 - Psyr_0257 0.87 +0.0
273,521 - Psyr_0257 0.87 -1.3
273,521 - Psyr_0257 0.87 -1.9
273,521 - Psyr_0257 0.87 -1.0
273,529 + Psyr_0257 0.87 -0.9
273,529 + Psyr_0257 0.87 +0.2
273,529 + Psyr_0257 0.87 +0.1
273,529 + Psyr_0257 0.87 -0.8
273,529 + Psyr_0257 0.87 -0.1
273,530 - Psyr_0257 0.87 -0.8
273,530 - Psyr_0257 0.87 -1.1
273,530 - Psyr_0257 0.87 +0.6
273,530 - Psyr_0257 0.87 -0.3
273,656 - -0.1
273,656 - -0.9
273,760 - +0.4
273,793 - +0.6
273,834 - +0.4
273,868 + -0.3
273,868 + -0.5
273,868 + -2.2
273,868 + +1.8
273,868 + -0.5
273,868 + +0.5
273,868 + -0.5
273,868 + +0.0
273,868 + -0.1
273,869 - +0.2
273,869 - +0.6
273,869 - -1.0
273,869 - -1.0
273,869 - -1.1
273,872 + +0.2
273,872 + +0.5
273,872 + -1.0
273,872 + -1.4
273,873 - -0.4
273,873 - +0.4
273,909 + +0.4
273,970 + -0.6
273,970 + -0.0
273,971 - +1.1
273,973 - +0.5
273,990 + +0.1
274,096 + -1.6
274,096 + -0.1

Or see this region's nucleotide sequence