Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_637 and Pf6N2E2_638 are separated by 195 nucleotides Pf6N2E2_638 and Pf6N2E2_639 are separated by 180 nucleotides Pf6N2E2_639 and Pf6N2E2_640 are separated by 205 nucleotides
Pf6N2E2_637 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 728,851 to 730,284
_637
Pf6N2E2_638 - 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein, at 730,480 to 730,920
_638
Pf6N2E2_639 - FIG01285020: hypothetical protein, at 731,101 to 731,442
_639
Pf6N2E2_640 - Outer membrane TonB-dependent transducer VreA of trans-envelope signaling system, at 731,648 to 732,280
_640
Position (kb) 730
731 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 729.531 kb on - strand, within Pf6N2E2_637 at 729.825 kb on - strand, within Pf6N2E2_637 at 729.825 kb on - strand, within Pf6N2E2_637 at 730.061 kb on + strand, within Pf6N2E2_637 at 730.061 kb on + strand, within Pf6N2E2_637 at 730.061 kb on + strand, within Pf6N2E2_637 at 730.062 kb on - strand, within Pf6N2E2_637 at 730.062 kb on - strand, within Pf6N2E2_637 at 730.101 kb on - strand, within Pf6N2E2_637 at 730.101 kb on - strand, within Pf6N2E2_637 at 730.115 kb on + strand, within Pf6N2E2_637 at 730.116 kb on - strand, within Pf6N2E2_637 at 730.232 kb on + strand at 730.233 kb on - strand at 730.233 kb on - strand at 730.233 kb on - strand at 730.358 kb on + strand at 730.359 kb on - strand at 730.426 kb on + strand at 730.498 kb on + strand at 730.498 kb on + strand at 730.501 kb on + strand at 730.501 kb on + strand at 730.502 kb on - strand at 730.502 kb on - strand at 730.502 kb on - strand at 730.502 kb on - strand at 730.502 kb on - strand at 730.801 kb on + strand, within Pf6N2E2_638 at 730.801 kb on + strand, within Pf6N2E2_638 at 730.802 kb on - strand, within Pf6N2E2_638 at 730.802 kb on - strand, within Pf6N2E2_638 at 731.029 kb on - strand at 731.103 kb on - strand at 731.169 kb on + strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.170 kb on - strand, within Pf6N2E2_639 at 731.495 kb on + strand at 731.528 kb on + strand at 731.528 kb on + strand at 731.529 kb on - strand at 731.540 kb on - strand at 731.540 kb on - strand at 731.572 kb on + strand at 731.573 kb on - strand at 731.573 kb on - strand at 731.803 kb on + strand, within Pf6N2E2_640 at 731.803 kb on + strand, within Pf6N2E2_640 at 731.804 kb on - strand, within Pf6N2E2_640 at 731.882 kb on + strand, within Pf6N2E2_640
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 729,531 - Pf6N2E2_637 0.47 +1.1 729,825 - Pf6N2E2_637 0.68 -0.2 729,825 - Pf6N2E2_637 0.68 +0.4 730,061 + Pf6N2E2_637 0.84 +0.5 730,061 + Pf6N2E2_637 0.84 -1.5 730,061 + Pf6N2E2_637 0.84 +0.5 730,062 - Pf6N2E2_637 0.84 -1.5 730,062 - Pf6N2E2_637 0.84 -2.8 730,101 - Pf6N2E2_637 0.87 +1.1 730,101 - Pf6N2E2_637 0.87 -1.4 730,115 + Pf6N2E2_637 0.88 -1.4 730,116 - Pf6N2E2_637 0.88 +0.9 730,232 + +0.7 730,233 - -1.0 730,233 - -2.0 730,233 - -1.8 730,358 + +1.7 730,359 - +0.5 730,426 + -1.0 730,498 + -1.1 730,498 + -0.5 730,501 + -0.8 730,501 + +0.7 730,502 - -1.1 730,502 - +0.4 730,502 - +0.9 730,502 - +1.4 730,502 - +2.5 730,801 + Pf6N2E2_638 0.73 -0.6 730,801 + Pf6N2E2_638 0.73 -1.4 730,802 - Pf6N2E2_638 0.73 -1.1 730,802 - Pf6N2E2_638 0.73 -1.8 731,029 - +0.2 731,103 - +0.5 731,169 + Pf6N2E2_639 0.20 -0.0 731,170 - Pf6N2E2_639 0.20 +0.6 731,170 - Pf6N2E2_639 0.20 +0.5 731,170 - Pf6N2E2_639 0.20 +0.6 731,170 - Pf6N2E2_639 0.20 -0.2 731,495 + -2.3 731,528 + -2.1 731,528 + -0.3 731,529 - +2.5 731,540 - -0.3 731,540 - -0.8 731,572 + -4.0 731,573 - +0.8 731,573 - +1.0 731,803 + Pf6N2E2_640 0.24 +0.7 731,803 + Pf6N2E2_640 0.24 +0.5 731,804 - Pf6N2E2_640 0.25 +0.4 731,882 + Pf6N2E2_640 0.37 -0.1
Or see this region's nucleotide sequence