Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6017 and Pf6N2E2_6018 are separated by 102 nucleotides Pf6N2E2_6018 and Pf6N2E2_6019 are separated by 21 nucleotides Pf6N2E2_6019 and Pf6N2E2_6020 are separated by 194 nucleotides
Pf6N2E2_6017 - Hexuronate transporter, at 6,837,012 to 6,838,343
_6017
Pf6N2E2_6018 - Fumarylacetoacetate hydrolase family protein, at 6,838,446 to 6,839,144
_6018
Pf6N2E2_6019 - putative oxidoreductase, at 6,839,166 to 6,839,924
_6019
Pf6N2E2_6020 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 6,840,119 to 6,841,075
_6020
Position (kb)
6838
6839
6840 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6837.480 kb on + strand, within Pf6N2E2_6017 at 6837.481 kb on - strand, within Pf6N2E2_6017 at 6837.481 kb on - strand, within Pf6N2E2_6017 at 6837.495 kb on + strand, within Pf6N2E2_6017 at 6837.495 kb on + strand, within Pf6N2E2_6017 at 6837.495 kb on + strand, within Pf6N2E2_6017 at 6837.546 kb on + strand, within Pf6N2E2_6017 at 6837.546 kb on + strand, within Pf6N2E2_6017 at 6837.546 kb on + strand, within Pf6N2E2_6017 at 6837.546 kb on + strand, within Pf6N2E2_6017 at 6837.547 kb on - strand, within Pf6N2E2_6017 at 6837.547 kb on - strand, within Pf6N2E2_6017 at 6837.547 kb on - strand, within Pf6N2E2_6017 at 6837.547 kb on - strand, within Pf6N2E2_6017 at 6837.547 kb on - strand, within Pf6N2E2_6017 at 6837.567 kb on + strand, within Pf6N2E2_6017 at 6837.567 kb on + strand, within Pf6N2E2_6017 at 6837.567 kb on + strand, within Pf6N2E2_6017 at 6837.567 kb on + strand, within Pf6N2E2_6017 at 6837.568 kb on - strand, within Pf6N2E2_6017 at 6837.619 kb on - strand, within Pf6N2E2_6017 at 6837.667 kb on - strand, within Pf6N2E2_6017 at 6837.667 kb on - strand, within Pf6N2E2_6017 at 6837.667 kb on - strand, within Pf6N2E2_6017 at 6837.667 kb on - strand, within Pf6N2E2_6017 at 6837.667 kb on - strand, within Pf6N2E2_6017 at 6837.667 kb on - strand, within Pf6N2E2_6017 at 6837.751 kb on - strand, within Pf6N2E2_6017 at 6837.804 kb on + strand, within Pf6N2E2_6017 at 6837.805 kb on - strand, within Pf6N2E2_6017 at 6837.805 kb on - strand, within Pf6N2E2_6017 at 6837.805 kb on - strand, within Pf6N2E2_6017 at 6837.832 kb on - strand, within Pf6N2E2_6017 at 6837.832 kb on - strand, within Pf6N2E2_6017 at 6838.166 kb on + strand, within Pf6N2E2_6017 at 6838.166 kb on + strand, within Pf6N2E2_6017 at 6838.167 kb on - strand, within Pf6N2E2_6017 at 6838.294 kb on - strand at 6838.294 kb on - strand at 6838.317 kb on + strand at 6838.414 kb on - strand at 6838.440 kb on - strand at 6838.461 kb on + strand at 6838.462 kb on - strand at 6838.462 kb on - strand at 6838.462 kb on - strand at 6838.554 kb on + strand, within Pf6N2E2_6018 at 6838.746 kb on + strand, within Pf6N2E2_6018 at 6838.747 kb on - strand, within Pf6N2E2_6018 at 6838.747 kb on - strand, within Pf6N2E2_6018 at 6838.747 kb on - strand, within Pf6N2E2_6018 at 6838.747 kb on - strand, within Pf6N2E2_6018 at 6838.747 kb on - strand, within Pf6N2E2_6018 at 6838.921 kb on + strand, within Pf6N2E2_6018 at 6839.139 kb on + strand at 6839.139 kb on + strand at 6839.253 kb on + strand, within Pf6N2E2_6019 at 6839.353 kb on + strand, within Pf6N2E2_6019 at 6839.354 kb on - strand, within Pf6N2E2_6019 at 6839.354 kb on - strand, within Pf6N2E2_6019 at 6839.354 kb on - strand, within Pf6N2E2_6019 at 6839.403 kb on + strand, within Pf6N2E2_6019 at 6839.404 kb on - strand, within Pf6N2E2_6019 at 6839.697 kb on + strand, within Pf6N2E2_6019 at 6839.697 kb on + strand, within Pf6N2E2_6019 at 6839.697 kb on + strand, within Pf6N2E2_6019 at 6839.698 kb on - strand, within Pf6N2E2_6019 at 6839.698 kb on - strand, within Pf6N2E2_6019 at 6839.698 kb on - strand, within Pf6N2E2_6019 at 6839.698 kb on - strand, within Pf6N2E2_6019 at 6839.770 kb on + strand, within Pf6N2E2_6019 at 6839.770 kb on + strand, within Pf6N2E2_6019 at 6839.771 kb on - strand, within Pf6N2E2_6019 at 6839.842 kb on - strand, within Pf6N2E2_6019 at 6840.107 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 6,837,480 + Pf6N2E2_6017 0.35 +1.5 6,837,481 - Pf6N2E2_6017 0.35 -0.5 6,837,481 - Pf6N2E2_6017 0.35 +0.9 6,837,495 + Pf6N2E2_6017 0.36 -1.2 6,837,495 + Pf6N2E2_6017 0.36 -2.8 6,837,495 + Pf6N2E2_6017 0.36 -0.1 6,837,546 + Pf6N2E2_6017 0.40 +0.9 6,837,546 + Pf6N2E2_6017 0.40 -0.1 6,837,546 + Pf6N2E2_6017 0.40 +1.0 6,837,546 + Pf6N2E2_6017 0.40 +0.7 6,837,547 - Pf6N2E2_6017 0.40 -0.2 6,837,547 - Pf6N2E2_6017 0.40 +0.1 6,837,547 - Pf6N2E2_6017 0.40 -0.6 6,837,547 - Pf6N2E2_6017 0.40 +0.9 6,837,547 - Pf6N2E2_6017 0.40 +0.7 6,837,567 + Pf6N2E2_6017 0.42 -1.9 6,837,567 + Pf6N2E2_6017 0.42 +1.2 6,837,567 + Pf6N2E2_6017 0.42 +0.5 6,837,567 + Pf6N2E2_6017 0.42 +0.8 6,837,568 - Pf6N2E2_6017 0.42 +0.2 6,837,619 - Pf6N2E2_6017 0.46 +0.5 6,837,667 - Pf6N2E2_6017 0.49 -1.7 6,837,667 - Pf6N2E2_6017 0.49 +0.9 6,837,667 - Pf6N2E2_6017 0.49 +2.0 6,837,667 - Pf6N2E2_6017 0.49 +1.0 6,837,667 - Pf6N2E2_6017 0.49 -1.3 6,837,667 - Pf6N2E2_6017 0.49 -0.5 6,837,751 - Pf6N2E2_6017 0.55 +1.5 6,837,804 + Pf6N2E2_6017 0.59 +0.6 6,837,805 - Pf6N2E2_6017 0.60 -0.2 6,837,805 - Pf6N2E2_6017 0.60 +1.4 6,837,805 - Pf6N2E2_6017 0.60 -1.4 6,837,832 - Pf6N2E2_6017 0.62 -0.2 6,837,832 - Pf6N2E2_6017 0.62 +1.7 6,838,166 + Pf6N2E2_6017 0.87 -0.2 6,838,166 + Pf6N2E2_6017 0.87 +0.3 6,838,167 - Pf6N2E2_6017 0.87 -0.4 6,838,294 - +1.8 6,838,294 - +0.8 6,838,317 + +0.0 6,838,414 - +1.5 6,838,440 - +0.2 6,838,461 + +0.1 6,838,462 - +0.4 6,838,462 - -0.4 6,838,462 - -0.1 6,838,554 + Pf6N2E2_6018 0.15 -1.4 6,838,746 + Pf6N2E2_6018 0.43 +0.6 6,838,747 - Pf6N2E2_6018 0.43 +0.0 6,838,747 - Pf6N2E2_6018 0.43 +1.1 6,838,747 - Pf6N2E2_6018 0.43 -0.1 6,838,747 - Pf6N2E2_6018 0.43 +1.1 6,838,747 - Pf6N2E2_6018 0.43 +1.3 6,838,921 + Pf6N2E2_6018 0.68 +1.0 6,839,139 + +0.5 6,839,139 + +0.6 6,839,253 + Pf6N2E2_6019 0.11 -0.4 6,839,353 + Pf6N2E2_6019 0.25 +0.4 6,839,354 - Pf6N2E2_6019 0.25 +1.9 6,839,354 - Pf6N2E2_6019 0.25 -0.7 6,839,354 - Pf6N2E2_6019 0.25 +0.7 6,839,403 + Pf6N2E2_6019 0.31 +0.2 6,839,404 - Pf6N2E2_6019 0.31 -1.1 6,839,697 + Pf6N2E2_6019 0.70 -0.7 6,839,697 + Pf6N2E2_6019 0.70 -2.3 6,839,697 + Pf6N2E2_6019 0.70 -0.8 6,839,698 - Pf6N2E2_6019 0.70 +1.3 6,839,698 - Pf6N2E2_6019 0.70 +0.6 6,839,698 - Pf6N2E2_6019 0.70 -0.2 6,839,698 - Pf6N2E2_6019 0.70 +0.2 6,839,770 + Pf6N2E2_6019 0.80 -3.1 6,839,770 + Pf6N2E2_6019 0.80 +1.2 6,839,771 - Pf6N2E2_6019 0.80 +1.1 6,839,842 - Pf6N2E2_6019 0.89 +0.1 6,840,107 + +0.3
Or see this region's nucleotide sequence