Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4919 and Pf6N2E2_4920 are separated by 46 nucleotides Pf6N2E2_4920 and Pf6N2E2_4921 are separated by 16 nucleotides Pf6N2E2_4921 and Pf6N2E2_4922 are separated by 301 nucleotides Pf6N2E2_4922 and Pf6N2E2_4923 overlap by 4 nucleotides
Pf6N2E2_4919 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 5,673,324 to 5,674,457
_4919
Pf6N2E2_4920 - Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9), at 5,674,504 to 5,675,169
_4920
Pf6N2E2_4921 - 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25), at 5,675,186 to 5,676,277
_4921
Pf6N2E2_4922 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 5,676,579 to 5,677,055
_4922
Pf6N2E2_4923 - Transcription termination protein NusB, at 5,677,052 to 5,677,552
_4923
Position (kb)
5675
5676
5677 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 5674.453 kb on + strand at 5674.453 kb on + strand at 5674.453 kb on + strand at 5674.453 kb on + strand at 5674.454 kb on - strand at 5674.454 kb on - strand at 5674.454 kb on - strand at 5674.454 kb on - strand at 5674.455 kb on + strand at 5674.455 kb on + strand at 5675.155 kb on + strand at 5675.197 kb on + strand at 5675.197 kb on + strand at 5675.197 kb on + strand at 5675.198 kb on - strand at 5675.198 kb on - strand at 5675.479 kb on + strand, within Pf6N2E2_4921 at 5675.798 kb on - strand, within Pf6N2E2_4921 at 5676.140 kb on + strand, within Pf6N2E2_4921 at 5676.140 kb on + strand, within Pf6N2E2_4921 at 5676.140 kb on + strand, within Pf6N2E2_4921 at 5676.140 kb on + strand, within Pf6N2E2_4921 at 5676.140 kb on + strand, within Pf6N2E2_4921 at 5676.140 kb on + strand, within Pf6N2E2_4921 at 5676.190 kb on + strand at 5676.223 kb on + strand at 5676.223 kb on + strand at 5676.255 kb on + strand at 5676.260 kb on + strand at 5676.260 kb on + strand at 5676.260 kb on + strand at 5676.260 kb on + strand at 5676.260 kb on + strand at 5676.260 kb on + strand at 5676.298 kb on + strand at 5676.298 kb on + strand at 5676.298 kb on + strand at 5676.298 kb on + strand at 5676.319 kb on + strand at 5676.319 kb on + strand at 5676.319 kb on + strand at 5676.319 kb on + strand at 5676.319 kb on + strand at 5676.319 kb on + strand at 5676.319 kb on + strand at 5676.398 kb on + strand at 5676.510 kb on + strand at 5676.510 kb on + strand at 5676.522 kb on + strand at 5676.522 kb on + strand at 5676.522 kb on + strand at 5676.522 kb on + strand at 5676.522 kb on + strand at 5676.525 kb on + strand at 5676.525 kb on + strand at 5676.544 kb on + strand at 5676.564 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 5,674,453 + +0.6 5,674,453 + +1.0 5,674,453 + -0.6 5,674,453 + +1.1 5,674,454 - +0.5 5,674,454 - +0.7 5,674,454 - -0.7 5,674,454 - -0.2 5,674,455 + +4.7 5,674,455 + -1.6 5,675,155 + +0.2 5,675,197 + +0.0 5,675,197 + +0.3 5,675,197 + -1.0 5,675,198 - -0.7 5,675,198 - +0.2 5,675,479 + Pf6N2E2_4921 0.27 -1.0 5,675,798 - Pf6N2E2_4921 0.56 +1.7 5,676,140 + Pf6N2E2_4921 0.87 +1.4 5,676,140 + Pf6N2E2_4921 0.87 +1.9 5,676,140 + Pf6N2E2_4921 0.87 +0.8 5,676,140 + Pf6N2E2_4921 0.87 +0.6 5,676,140 + Pf6N2E2_4921 0.87 +1.4 5,676,140 + Pf6N2E2_4921 0.87 +1.0 5,676,190 + -0.2 5,676,223 + -2.1 5,676,223 + +1.6 5,676,255 + -1.0 5,676,260 + -0.5 5,676,260 + -0.5 5,676,260 + +0.7 5,676,260 + +0.5 5,676,260 + +0.6 5,676,260 + +0.6 5,676,298 + +0.9 5,676,298 + -0.6 5,676,298 + +1.0 5,676,298 + +0.5 5,676,319 + -1.2 5,676,319 + +0.9 5,676,319 + +0.4 5,676,319 + -0.2 5,676,319 + -1.2 5,676,319 + +1.0 5,676,319 + +0.6 5,676,398 + -0.7 5,676,510 + +0.1 5,676,510 + +0.9 5,676,522 + +0.8 5,676,522 + +0.5 5,676,522 + +0.3 5,676,522 + -0.2 5,676,522 + +0.6 5,676,525 + -0.5 5,676,525 + +0.4 5,676,544 + +1.4 5,676,564 + -0.2
Or see this region's nucleotide sequence