Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3820

Experiment: RCH2_defined

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3819 and Pf6N2E2_3820 are separated by 166 nucleotidesPf6N2E2_3820 and Pf6N2E2_3821 overlap by 33 nucleotides Pf6N2E2_3819 - Thiamine biosynthesis protein thiI, at 4,454,493 to 4,455,947 _3819 Pf6N2E2_3820 - hypothetical protein, at 4,456,114 to 4,456,308 _3820 Pf6N2E2_3821 - Glutamine synthetase type I (EC 6.3.1.2), at 4,456,276 to 4,457,682 _3821 Position (kb) 4456 4457Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4455.993 kb on - strandat 4456.094 kb on - strandat 4456.115 kb on + strandat 4456.115 kb on + strandat 4456.116 kb on - strandat 4456.209 kb on + strand, within Pf6N2E2_3820at 4456.230 kb on + strand, within Pf6N2E2_3820at 4456.234 kb on + strand, within Pf6N2E2_3820at 4456.234 kb on + strand, within Pf6N2E2_3820at 4456.275 kb on + strand, within Pf6N2E2_3820

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2_defined
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4,455,993 - -0.1
4,456,094 - -2.6
4,456,115 + -0.5
4,456,115 + -1.5
4,456,116 - -4.4
4,456,209 + Pf6N2E2_3820 0.49 -2.6
4,456,230 + Pf6N2E2_3820 0.59 +2.6
4,456,234 + Pf6N2E2_3820 0.62 -3.3
4,456,234 + Pf6N2E2_3820 0.62 -0.4
4,456,275 + Pf6N2E2_3820 0.83 +1.6

Or see this region's nucleotide sequence