Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2226 and Pf6N2E2_2227 are separated by 26 nucleotides Pf6N2E2_2227 and Pf6N2E2_2228 are separated by 139 nucleotides Pf6N2E2_2228 and Pf6N2E2_2229 overlap by 4 nucleotides
Pf6N2E2_2226 - Integral membrane protein TerC, at 2,639,182 to 2,639,931
_2226
Pf6N2E2_2227 - Membrane protein involved in colicin uptake, at 2,639,958 to 2,640,209
_2227
Pf6N2E2_2228 - Transcriptional regulator, LysR family, at 2,640,349 to 2,641,245
_2228
Pf6N2E2_2229 - Major facilitator family transporter, at 2,641,242 to 2,642,393
_2229
Position (kb)
2640
2641
2642 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2639.917 kb on + strand at 2639.917 kb on + strand at 2639.918 kb on - strand at 2639.924 kb on + strand at 2639.924 kb on + strand at 2639.924 kb on + strand at 2639.924 kb on + strand at 2639.925 kb on - strand at 2639.925 kb on - strand at 2639.925 kb on - strand at 2639.925 kb on - strand at 2639.925 kb on - strand at 2639.959 kb on + strand at 2639.959 kb on + strand at 2639.959 kb on + strand at 2639.959 kb on + strand at 2639.960 kb on - strand at 2639.960 kb on - strand at 2640.202 kb on + strand at 2640.202 kb on + strand at 2640.202 kb on + strand at 2640.202 kb on + strand at 2640.202 kb on + strand at 2640.203 kb on - strand at 2640.203 kb on - strand at 2640.203 kb on - strand at 2640.233 kb on + strand at 2640.274 kb on + strand at 2640.274 kb on + strand at 2640.274 kb on + strand at 2640.274 kb on + strand at 2640.275 kb on - strand at 2640.275 kb on - strand at 2640.275 kb on - strand at 2640.373 kb on + strand at 2640.374 kb on - strand at 2640.374 kb on - strand at 2640.374 kb on - strand at 2640.390 kb on + strand at 2640.478 kb on + strand, within Pf6N2E2_2228 at 2640.478 kb on + strand, within Pf6N2E2_2228 at 2640.478 kb on + strand, within Pf6N2E2_2228 at 2640.478 kb on + strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.480 kb on + strand, within Pf6N2E2_2228 at 2640.481 kb on - strand, within Pf6N2E2_2228 at 2640.890 kb on - strand, within Pf6N2E2_2228 at 2641.006 kb on - strand, within Pf6N2E2_2228 at 2641.118 kb on + strand, within Pf6N2E2_2228 at 2641.118 kb on + strand, within Pf6N2E2_2228 at 2641.118 kb on + strand, within Pf6N2E2_2228 at 2641.119 kb on - strand, within Pf6N2E2_2228 at 2641.160 kb on - strand at 2641.160 kb on - strand at 2641.169 kb on - strand at 2641.243 kb on + strand at 2641.243 kb on + strand at 2641.244 kb on - strand at 2641.347 kb on - strand at 2641.644 kb on + strand, within Pf6N2E2_2229 at 2641.729 kb on + strand, within Pf6N2E2_2229 at 2641.729 kb on + strand, within Pf6N2E2_2229 at 2641.730 kb on - strand, within Pf6N2E2_2229 at 2641.736 kb on - strand, within Pf6N2E2_2229 at 2641.736 kb on - strand, within Pf6N2E2_2229 at 2641.736 kb on - strand, within Pf6N2E2_2229 at 2641.865 kb on - strand, within Pf6N2E2_2229 at 2641.865 kb on - strand, within Pf6N2E2_2229 at 2641.865 kb on - strand, within Pf6N2E2_2229 at 2641.868 kb on + strand, within Pf6N2E2_2229 at 2641.868 kb on + strand, within Pf6N2E2_2229 at 2641.869 kb on - strand, within Pf6N2E2_2229 at 2641.869 kb on - strand, within Pf6N2E2_2229 at 2642.180 kb on + strand, within Pf6N2E2_2229 at 2642.180 kb on + strand, within Pf6N2E2_2229
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 2,639,917 + +0.1 2,639,917 + -0.2 2,639,918 - -1.5 2,639,924 + +0.2 2,639,924 + -0.8 2,639,924 + -1.0 2,639,924 + +0.1 2,639,925 - -0.2 2,639,925 - +0.8 2,639,925 - -0.2 2,639,925 - -1.2 2,639,925 - -0.7 2,639,959 + -0.8 2,639,959 + -0.4 2,639,959 + +0.2 2,639,959 + -0.3 2,639,960 - +0.5 2,639,960 - +0.6 2,640,202 + +1.8 2,640,202 + +2.2 2,640,202 + +0.4 2,640,202 + -2.0 2,640,202 + -0.1 2,640,203 - -0.4 2,640,203 - +0.0 2,640,203 - +0.4 2,640,233 + -0.5 2,640,274 + -2.0 2,640,274 + -0.2 2,640,274 + -0.5 2,640,274 + -1.2 2,640,275 - -1.2 2,640,275 - +0.3 2,640,275 - -0.3 2,640,373 + -0.2 2,640,374 - -0.2 2,640,374 - +1.1 2,640,374 - -0.2 2,640,390 + -0.2 2,640,478 + Pf6N2E2_2228 0.14 +1.1 2,640,478 + Pf6N2E2_2228 0.14 -0.8 2,640,478 + Pf6N2E2_2228 0.14 +0.5 2,640,478 + Pf6N2E2_2228 0.14 -1.9 2,640,479 - Pf6N2E2_2228 0.14 -1.8 2,640,479 - Pf6N2E2_2228 0.14 +0.9 2,640,479 - Pf6N2E2_2228 0.14 +0.8 2,640,479 - Pf6N2E2_2228 0.14 +0.8 2,640,479 - Pf6N2E2_2228 0.14 +1.4 2,640,480 + Pf6N2E2_2228 0.15 +2.8 2,640,481 - Pf6N2E2_2228 0.15 -0.4 2,640,890 - Pf6N2E2_2228 0.60 +3.3 2,641,006 - Pf6N2E2_2228 0.73 -1.5 2,641,118 + Pf6N2E2_2228 0.86 +1.4 2,641,118 + Pf6N2E2_2228 0.86 +0.9 2,641,118 + Pf6N2E2_2228 0.86 +0.6 2,641,119 - Pf6N2E2_2228 0.86 +0.6 2,641,160 - -0.8 2,641,160 - -0.2 2,641,169 - -0.9 2,641,243 + -1.8 2,641,243 + +1.1 2,641,244 - +0.3 2,641,347 - +0.6 2,641,644 + Pf6N2E2_2229 0.35 -0.2 2,641,729 + Pf6N2E2_2229 0.42 -0.9 2,641,729 + Pf6N2E2_2229 0.42 -1.2 2,641,730 - Pf6N2E2_2229 0.42 +1.5 2,641,736 - Pf6N2E2_2229 0.43 +0.2 2,641,736 - Pf6N2E2_2229 0.43 -0.2 2,641,736 - Pf6N2E2_2229 0.43 +0.1 2,641,865 - Pf6N2E2_2229 0.54 -0.2 2,641,865 - Pf6N2E2_2229 0.54 -1.2 2,641,865 - Pf6N2E2_2229 0.54 +1.3 2,641,868 + Pf6N2E2_2229 0.54 -1.1 2,641,868 + Pf6N2E2_2229 0.54 +0.8 2,641,869 - Pf6N2E2_2229 0.54 -0.3 2,641,869 - Pf6N2E2_2229 0.54 +0.7 2,642,180 + Pf6N2E2_2229 0.81 +0.1 2,642,180 + Pf6N2E2_2229 0.81 +3.4
Or see this region's nucleotide sequence