Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1933 and Pf6N2E2_1934 are separated by 55 nucleotides Pf6N2E2_1934 and Pf6N2E2_1935 are separated by 63 nucleotides Pf6N2E2_1935 and Pf6N2E2_1936 are separated by 142 nucleotides
Pf6N2E2_1933 - TcuR: regulates tcuABC genes used in utilization of tricarballylate, at 2,289,767 to 2,290,699
_1933
Pf6N2E2_1934 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,290,755 to 2,291,549
_1934
Pf6N2E2_1935 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,291,613 to 2,292,257
_1935
Pf6N2E2_1936 - 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases / CDP-6-deoxy-delta-3,4-glucoseen reductase-like, at 2,292,400 to 2,293,440
_1936
Position (kb)
2291
2292
2293 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2290.664 kb on + strand at 2290.713 kb on + strand at 2290.713 kb on + strand at 2290.757 kb on - strand at 2290.792 kb on + strand at 2290.792 kb on + strand at 2290.793 kb on - strand at 2290.814 kb on - strand at 2290.814 kb on - strand at 2290.814 kb on - strand at 2290.814 kb on - strand at 2290.894 kb on + strand, within Pf6N2E2_1934 at 2290.895 kb on - strand, within Pf6N2E2_1934 at 2291.010 kb on + strand, within Pf6N2E2_1934 at 2291.011 kb on - strand, within Pf6N2E2_1934 at 2291.056 kb on - strand, within Pf6N2E2_1934 at 2291.056 kb on - strand, within Pf6N2E2_1934 at 2291.083 kb on - strand, within Pf6N2E2_1934 at 2291.098 kb on + strand, within Pf6N2E2_1934 at 2291.099 kb on - strand, within Pf6N2E2_1934 at 2291.135 kb on + strand, within Pf6N2E2_1934 at 2291.136 kb on - strand, within Pf6N2E2_1934 at 2291.136 kb on - strand, within Pf6N2E2_1934 at 2291.137 kb on + strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.138 kb on - strand, within Pf6N2E2_1934 at 2291.266 kb on - strand, within Pf6N2E2_1934 at 2291.304 kb on - strand, within Pf6N2E2_1934 at 2291.500 kb on - strand at 2291.509 kb on - strand at 2291.529 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.553 kb on - strand at 2291.790 kb on + strand, within Pf6N2E2_1935 at 2291.790 kb on + strand, within Pf6N2E2_1935 at 2291.791 kb on - strand, within Pf6N2E2_1935 at 2291.889 kb on + strand, within Pf6N2E2_1935 at 2291.890 kb on - strand, within Pf6N2E2_1935 at 2292.234 kb on - strand at 2292.234 kb on - strand at 2292.234 kb on - strand at 2292.281 kb on + strand at 2292.282 kb on - strand at 2292.282 kb on - strand at 2292.334 kb on - strand at 2292.401 kb on + strand at 2292.521 kb on + strand, within Pf6N2E2_1936 at 2292.522 kb on - strand, within Pf6N2E2_1936 at 2292.522 kb on - strand, within Pf6N2E2_1936 at 2292.595 kb on + strand, within Pf6N2E2_1936 at 2292.596 kb on - strand, within Pf6N2E2_1936 at 2292.596 kb on - strand, within Pf6N2E2_1936 at 2292.710 kb on + strand, within Pf6N2E2_1936 at 2292.710 kb on + strand, within Pf6N2E2_1936 at 2292.719 kb on - strand, within Pf6N2E2_1936 at 2292.814 kb on - strand, within Pf6N2E2_1936 at 2292.841 kb on + strand, within Pf6N2E2_1936 at 2292.901 kb on - strand, within Pf6N2E2_1936 at 2292.914 kb on + strand, within Pf6N2E2_1936 at 2292.981 kb on + strand, within Pf6N2E2_1936 at 2293.007 kb on + strand, within Pf6N2E2_1936 at 2293.007 kb on + strand, within Pf6N2E2_1936 at 2293.007 kb on + strand, within Pf6N2E2_1936 at 2293.007 kb on + strand, within Pf6N2E2_1936 at 2293.008 kb on - strand, within Pf6N2E2_1936 at 2293.229 kb on - strand, within Pf6N2E2_1936
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 2,290,664 + +0.8 2,290,713 + +0.2 2,290,713 + +0.6 2,290,757 - -0.9 2,290,792 + +1.6 2,290,792 + -1.9 2,290,793 - +0.2 2,290,814 - +1.1 2,290,814 - +0.4 2,290,814 - +0.2 2,290,814 - -1.1 2,290,894 + Pf6N2E2_1934 0.17 -0.2 2,290,895 - Pf6N2E2_1934 0.18 +1.6 2,291,010 + Pf6N2E2_1934 0.32 +1.0 2,291,011 - Pf6N2E2_1934 0.32 +1.5 2,291,056 - Pf6N2E2_1934 0.38 +0.2 2,291,056 - Pf6N2E2_1934 0.38 -0.7 2,291,083 - Pf6N2E2_1934 0.41 -2.1 2,291,098 + Pf6N2E2_1934 0.43 +1.0 2,291,099 - Pf6N2E2_1934 0.43 +1.6 2,291,135 + Pf6N2E2_1934 0.48 +0.3 2,291,136 - Pf6N2E2_1934 0.48 -0.8 2,291,136 - Pf6N2E2_1934 0.48 -2.7 2,291,137 + Pf6N2E2_1934 0.48 -0.7 2,291,138 - Pf6N2E2_1934 0.48 -2.2 2,291,138 - Pf6N2E2_1934 0.48 +0.6 2,291,138 - Pf6N2E2_1934 0.48 +0.5 2,291,138 - Pf6N2E2_1934 0.48 +1.4 2,291,138 - Pf6N2E2_1934 0.48 -1.2 2,291,266 - Pf6N2E2_1934 0.64 +0.6 2,291,304 - Pf6N2E2_1934 0.69 -0.1 2,291,500 - -0.4 2,291,509 - +0.2 2,291,529 - -2.2 2,291,553 - -0.3 2,291,553 - +0.4 2,291,553 - +1.5 2,291,553 - -0.5 2,291,553 - +0.6 2,291,553 - -1.4 2,291,790 + Pf6N2E2_1935 0.27 -1.4 2,291,790 + Pf6N2E2_1935 0.27 -1.0 2,291,791 - Pf6N2E2_1935 0.28 -0.4 2,291,889 + Pf6N2E2_1935 0.43 -0.5 2,291,890 - Pf6N2E2_1935 0.43 -1.7 2,292,234 - +0.4 2,292,234 - -2.3 2,292,234 - +0.2 2,292,281 + +0.6 2,292,282 - +0.1 2,292,282 - +0.4 2,292,334 - -1.9 2,292,401 + -1.4 2,292,521 + Pf6N2E2_1936 0.12 -0.4 2,292,522 - Pf6N2E2_1936 0.12 -1.4 2,292,522 - Pf6N2E2_1936 0.12 +3.0 2,292,595 + Pf6N2E2_1936 0.19 -2.5 2,292,596 - Pf6N2E2_1936 0.19 +1.5 2,292,596 - Pf6N2E2_1936 0.19 +0.2 2,292,710 + Pf6N2E2_1936 0.30 -0.2 2,292,710 + Pf6N2E2_1936 0.30 +0.4 2,292,719 - Pf6N2E2_1936 0.31 +0.6 2,292,814 - Pf6N2E2_1936 0.40 +2.4 2,292,841 + Pf6N2E2_1936 0.42 +1.1 2,292,901 - Pf6N2E2_1936 0.48 +0.6 2,292,914 + Pf6N2E2_1936 0.49 -1.4 2,292,981 + Pf6N2E2_1936 0.56 +0.2 2,293,007 + Pf6N2E2_1936 0.58 -2.7 2,293,007 + Pf6N2E2_1936 0.58 +0.1 2,293,007 + Pf6N2E2_1936 0.58 -0.3 2,293,007 + Pf6N2E2_1936 0.58 +0.6 2,293,008 - Pf6N2E2_1936 0.58 -0.4 2,293,229 - Pf6N2E2_1936 0.80 -0.4
Or see this region's nucleotide sequence