Experiment: RCH2_defined
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1492 and Pf6N2E2_1493 are separated by 27 nucleotides Pf6N2E2_1493 and Pf6N2E2_1494 overlap by 14 nucleotides Pf6N2E2_1494 and Pf6N2E2_1495 overlap by 11 nucleotides Pf6N2E2_1495 and Pf6N2E2_1496 overlap by 4 nucleotides
Pf6N2E2_1492 - No significant database matches, at 1,746,523 to 1,747,122
_1492
Pf6N2E2_1493 - ABC-type polar amino acid transport system, ATPase component, at 1,747,150 to 1,747,986
_1493
Pf6N2E2_1494 - Glutamate transport membrane-spanning protein, at 1,747,973 to 1,748,761
_1494
Pf6N2E2_1495 - Glutamate transport membrane-spanning protein, at 1,748,751 to 1,749,485
_1495
Pf6N2E2_1496 - Aspartate aminotransferase (EC 2.6.1.1), at 1,749,482 to 1,750,693
_1496
Position (kb) 1747
1748
1749 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1747.117 kb on - strand at 1747.191 kb on - strand at 1747.191 kb on - strand at 1747.191 kb on - strand at 1747.243 kb on + strand, within Pf6N2E2_1493 at 1747.332 kb on - strand, within Pf6N2E2_1493 at 1747.382 kb on - strand, within Pf6N2E2_1493 at 1747.694 kb on + strand, within Pf6N2E2_1493 at 1747.694 kb on + strand, within Pf6N2E2_1493 at 1747.694 kb on + strand, within Pf6N2E2_1493 at 1747.694 kb on + strand, within Pf6N2E2_1493 at 1747.695 kb on - strand, within Pf6N2E2_1493 at 1747.695 kb on - strand, within Pf6N2E2_1493 at 1747.977 kb on + strand at 1748.008 kb on + strand at 1748.009 kb on - strand at 1748.031 kb on + strand at 1748.062 kb on - strand, within Pf6N2E2_1494 at 1748.062 kb on - strand, within Pf6N2E2_1494 at 1748.179 kb on - strand, within Pf6N2E2_1494 at 1748.179 kb on - strand, within Pf6N2E2_1494 at 1748.427 kb on + strand, within Pf6N2E2_1494 at 1748.427 kb on + strand, within Pf6N2E2_1494 at 1748.427 kb on + strand, within Pf6N2E2_1494 at 1748.427 kb on + strand, within Pf6N2E2_1494 at 1748.427 kb on + strand, within Pf6N2E2_1494 at 1748.427 kb on + strand, within Pf6N2E2_1494 at 1748.428 kb on - strand, within Pf6N2E2_1494 at 1748.428 kb on - strand, within Pf6N2E2_1494 at 1748.428 kb on - strand, within Pf6N2E2_1494 at 1748.439 kb on + strand, within Pf6N2E2_1494 at 1748.665 kb on - strand, within Pf6N2E2_1494 at 1748.665 kb on - strand, within Pf6N2E2_1494 at 1748.716 kb on + strand at 1748.899 kb on + strand, within Pf6N2E2_1495 at 1748.899 kb on + strand, within Pf6N2E2_1495 at 1748.900 kb on - strand, within Pf6N2E2_1495 at 1748.900 kb on - strand, within Pf6N2E2_1495 at 1748.900 kb on - strand, within Pf6N2E2_1495 at 1749.223 kb on + strand, within Pf6N2E2_1495 at 1749.224 kb on - strand, within Pf6N2E2_1495 at 1749.224 kb on - strand, within Pf6N2E2_1495 at 1749.224 kb on - strand, within Pf6N2E2_1495 at 1749.224 kb on - strand, within Pf6N2E2_1495 at 1749.409 kb on + strand, within Pf6N2E2_1495 at 1749.431 kb on - strand at 1749.592 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined remove 1,747,117 - +1.1 1,747,191 - +1.4 1,747,191 - +1.4 1,747,191 - +0.6 1,747,243 + Pf6N2E2_1493 0.11 -2.8 1,747,332 - Pf6N2E2_1493 0.22 +1.1 1,747,382 - Pf6N2E2_1493 0.28 -1.1 1,747,694 + Pf6N2E2_1493 0.65 +1.0 1,747,694 + Pf6N2E2_1493 0.65 -1.2 1,747,694 + Pf6N2E2_1493 0.65 +0.7 1,747,694 + Pf6N2E2_1493 0.65 -0.5 1,747,695 - Pf6N2E2_1493 0.65 -0.3 1,747,695 - Pf6N2E2_1493 0.65 +2.0 1,747,977 + -1.9 1,748,008 + +0.7 1,748,009 - -0.6 1,748,031 + -0.7 1,748,062 - Pf6N2E2_1494 0.11 +0.2 1,748,062 - Pf6N2E2_1494 0.11 +0.7 1,748,179 - Pf6N2E2_1494 0.26 -1.9 1,748,179 - Pf6N2E2_1494 0.26 -0.9 1,748,427 + Pf6N2E2_1494 0.58 -0.7 1,748,427 + Pf6N2E2_1494 0.58 -0.3 1,748,427 + Pf6N2E2_1494 0.58 -1.2 1,748,427 + Pf6N2E2_1494 0.58 +0.4 1,748,427 + Pf6N2E2_1494 0.58 +0.4 1,748,427 + Pf6N2E2_1494 0.58 +1.6 1,748,428 - Pf6N2E2_1494 0.58 +0.4 1,748,428 - Pf6N2E2_1494 0.58 +1.4 1,748,428 - Pf6N2E2_1494 0.58 -1.0 1,748,439 + Pf6N2E2_1494 0.59 -1.9 1,748,665 - Pf6N2E2_1494 0.88 -1.4 1,748,665 - Pf6N2E2_1494 0.88 -0.1 1,748,716 + -0.6 1,748,899 + Pf6N2E2_1495 0.20 +0.9 1,748,899 + Pf6N2E2_1495 0.20 -1.6 1,748,900 - Pf6N2E2_1495 0.20 -0.6 1,748,900 - Pf6N2E2_1495 0.20 -1.0 1,748,900 - Pf6N2E2_1495 0.20 +1.0 1,749,223 + Pf6N2E2_1495 0.64 +0.1 1,749,224 - Pf6N2E2_1495 0.64 +1.0 1,749,224 - Pf6N2E2_1495 0.64 -0.1 1,749,224 - Pf6N2E2_1495 0.64 +1.0 1,749,224 - Pf6N2E2_1495 0.64 -0.2 1,749,409 + Pf6N2E2_1495 0.90 -0.6 1,749,431 - -0.3 1,749,592 - -1.6
Or see this region's nucleotide sequence