Experiment: Sucrose (C) and L-Glutamine (N); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS11840 and PFLU_RS11845 are separated by 37 nucleotides PFLU_RS11845 and PFLU_RS11850 are separated by 1 nucleotides PFLU_RS11850 and PFLU_RS11855 overlap by 4 nucleotides PFLU_RS11855 and PFLU_RS11860 are separated by 93 nucleotides PFLU_RS11860 and PFLU_RS11865 overlap by 14 nucleotides
PFLU_RS11840: PFLU_RS11840 - RNA polymerase sigma factor, at 2,624,296 to 2,624,841
_RS11840
PFLU_RS11845: PFLU_RS11845 - DUF4880 domain-containing protein, at 2,624,879 to 2,625,805
_RS11845
PFLU_RS11850: PFLU_RS11850 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 2,625,807 to 2,626,403
_RS11850
PFLU_RS11855: PFLU_RS11855 - type II secretion system protein GspH, at 2,626,400 to 2,626,852
_RS11855
PFLU_RS11860: PFLU_RS11860 - general secretion pathway protein GspC, at 2,626,946 to 2,627,377
_RS11860
PFLU_RS11865: PFLU_RS11865 - type II secretion system protein GspI, at 2,627,364 to 2,627,729
_RS11865
Position (kb)
2625
2626
2627 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2624.839 kb on + strand at 2624.839 kb on + strand at 2624.840 kb on - strand at 2624.857 kb on - strand at 2624.869 kb on - strand at 2624.869 kb on - strand at 2624.869 kb on - strand at 2624.869 kb on - strand at 2624.869 kb on - strand at 2625.180 kb on + strand, within PFLU_RS11845 at 2625.212 kb on + strand, within PFLU_RS11845 at 2625.433 kb on + strand, within PFLU_RS11845 at 2625.433 kb on + strand, within PFLU_RS11845 at 2625.442 kb on + strand, within PFLU_RS11845 at 2625.443 kb on - strand, within PFLU_RS11845 at 2625.522 kb on - strand, within PFLU_RS11845 at 2625.673 kb on + strand, within PFLU_RS11845 at 2625.755 kb on + strand at 2625.755 kb on + strand at 2625.755 kb on + strand at 2625.833 kb on + strand at 2625.833 kb on + strand at 2625.835 kb on + strand at 2625.835 kb on + strand at 2625.835 kb on + strand at 2625.835 kb on + strand at 2625.835 kb on + strand at 2625.835 kb on + strand at 2625.835 kb on + strand at 2625.836 kb on - strand at 2625.836 kb on - strand at 2625.836 kb on - strand at 2625.836 kb on - strand at 2625.836 kb on - strand at 2625.836 kb on - strand at 2625.836 kb on - strand at 2625.912 kb on + strand, within PFLU_RS11850 at 2625.913 kb on - strand, within PFLU_RS11850 at 2626.220 kb on + strand, within PFLU_RS11850 at 2626.220 kb on + strand, within PFLU_RS11850 at 2626.511 kb on - strand, within PFLU_RS11855 at 2626.857 kb on + strand at 2626.857 kb on + strand at 2626.857 kb on + strand at 2626.857 kb on + strand at 2626.858 kb on - strand at 2626.910 kb on + strand at 2626.911 kb on - strand at 2626.911 kb on - strand at 2626.963 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose (C) and L-Glutamine (N); with MOPS remove 2,624,839 + -0.0 2,624,839 + +0.3 2,624,840 - -1.4 2,624,857 - -0.6 2,624,869 - -0.7 2,624,869 - +0.1 2,624,869 - +1.0 2,624,869 - +0.2 2,624,869 - -0.3 2,625,180 + PFLU_RS11845 0.32 +1.3 2,625,212 + PFLU_RS11845 0.36 -1.1 2,625,433 + PFLU_RS11845 0.60 -0.3 2,625,433 + PFLU_RS11845 0.60 -0.0 2,625,442 + PFLU_RS11845 0.61 -0.8 2,625,443 - PFLU_RS11845 0.61 +1.4 2,625,522 - PFLU_RS11845 0.69 +0.6 2,625,673 + PFLU_RS11845 0.86 +0.3 2,625,755 + +0.1 2,625,755 + -0.7 2,625,755 + -1.7 2,625,833 + -0.2 2,625,833 + +2.0 2,625,835 + +0.7 2,625,835 + +0.2 2,625,835 + -0.4 2,625,835 + +0.6 2,625,835 + -0.6 2,625,835 + -1.3 2,625,835 + -0.7 2,625,836 - -0.4 2,625,836 - +1.2 2,625,836 - -0.5 2,625,836 - +0.8 2,625,836 - +0.4 2,625,836 - -0.7 2,625,836 - -1.4 2,625,912 + PFLU_RS11850 0.18 -1.9 2,625,913 - PFLU_RS11850 0.18 -0.4 2,626,220 + PFLU_RS11850 0.69 +0.8 2,626,220 + PFLU_RS11850 0.69 -0.3 2,626,511 - PFLU_RS11855 0.25 -0.2 2,626,857 + -0.1 2,626,857 + +0.5 2,626,857 + -1.2 2,626,857 + -2.1 2,626,858 - +0.7 2,626,910 + -0.9 2,626,911 - +1.0 2,626,911 - -0.2 2,626,963 + +0.3
Or see this region's nucleotide sequence