Experiment: Sucrose (C) and L-Glutamine (N); with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS03655 and PFLU_RS03660 are separated by 0 nucleotides PFLU_RS03660 and PFLU_RS03665 overlap by 7 nucleotides PFLU_RS03665 and PFLU_RS03670 overlap by 8 nucleotides PFLU_RS03670 and PFLU_RS03675 are separated by 112 nucleotides
PFLU_RS03655: PFLU_RS03655 - peptide chain release factor 1, at 839,042 to 840,124
_RS03655
PFLU_RS03660: PFLU_RS03660 - peptide chain release factor N(5)-glutamine methyltransferase, at 840,125 to 840,955
_RS03660
PFLU_RS03665: PFLU_RS03665 - molybdopterin-synthase adenylyltransferase MoeB, at 840,949 to 841,704
_RS03665
PFLU_RS03670: PFLU_RS03670 - glutamate racemase, at 841,697 to 842,491
_RS03670
PFLU_RS03675: PFLU_RS03675 - acyloxyacyl hydrolase, at 842,604 to 843,122
_RS03675
Position (kb) 840
841
842 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 840.089 kb on + strand at 840.114 kb on + strand at 840.114 kb on + strand at 840.114 kb on + strand at 840.122 kb on + strand at 840.122 kb on + strand at 840.122 kb on + strand at 840.122 kb on + strand at 840.976 kb on + strand at 841.087 kb on + strand, within PFLU_RS03665 at 841.462 kb on + strand, within PFLU_RS03665 at 841.462 kb on + strand, within PFLU_RS03665 at 841.462 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.472 kb on + strand, within PFLU_RS03665 at 841.473 kb on - strand, within PFLU_RS03665 at 841.474 kb on + strand, within PFLU_RS03665 at 841.474 kb on + strand, within PFLU_RS03665 at 842.451 kb on + strand at 842.451 kb on + strand at 842.451 kb on + strand at 842.459 kb on + strand at 842.515 kb on - strand at 842.548 kb on + strand at 842.548 kb on + strand at 842.548 kb on + strand at 842.548 kb on + strand at 842.558 kb on + strand at 842.558 kb on + strand at 842.558 kb on + strand at 842.559 kb on - strand at 842.559 kb on - strand at 842.575 kb on + strand at 842.575 kb on + strand at 842.576 kb on - strand at 842.576 kb on - strand at 842.577 kb on + strand at 842.577 kb on + strand at 842.577 kb on + strand at 842.577 kb on + strand at 842.577 kb on + strand at 842.577 kb on + strand at 842.577 kb on + strand at 842.577 kb on + strand at 842.577 kb on + strand at 842.577 kb on + strand at 842.578 kb on - strand at 842.578 kb on - strand at 842.578 kb on - strand at 842.578 kb on - strand at 842.578 kb on - strand at 842.578 kb on - strand at 842.578 kb on - strand at 842.578 kb on - strand at 842.578 kb on - strand at 842.578 kb on - strand at 842.578 kb on - strand at 842.578 kb on - strand at 842.578 kb on - strand at 842.604 kb on - strand at 842.644 kb on + strand at 842.644 kb on + strand at 842.644 kb on + strand at 842.644 kb on + strand at 842.644 kb on + strand at 842.644 kb on + strand at 842.645 kb on - strand at 842.645 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sucrose (C) and L-Glutamine (N); with MOPS remove 840,089 + -0.3 840,114 + +0.3 840,114 + -0.0 840,114 + -0.3 840,122 + -0.5 840,122 + -0.2 840,122 + +0.5 840,122 + +0.3 840,976 + -0.9 841,087 + PFLU_RS03665 0.18 -1.1 841,462 + PFLU_RS03665 0.68 +1.9 841,462 + PFLU_RS03665 0.68 -1.3 841,462 + PFLU_RS03665 0.68 +0.2 841,472 + PFLU_RS03665 0.69 -0.7 841,472 + PFLU_RS03665 0.69 -1.1 841,472 + PFLU_RS03665 0.69 -0.1 841,472 + PFLU_RS03665 0.69 -1.0 841,472 + PFLU_RS03665 0.69 -0.4 841,472 + PFLU_RS03665 0.69 +0.3 841,472 + PFLU_RS03665 0.69 +0.7 841,472 + PFLU_RS03665 0.69 -2.5 841,472 + PFLU_RS03665 0.69 -0.9 841,472 + PFLU_RS03665 0.69 -1.9 841,472 + PFLU_RS03665 0.69 -1.3 841,472 + PFLU_RS03665 0.69 +0.4 841,472 + PFLU_RS03665 0.69 +1.1 841,472 + PFLU_RS03665 0.69 +0.2 841,472 + PFLU_RS03665 0.69 -0.8 841,472 + PFLU_RS03665 0.69 -0.4 841,473 - PFLU_RS03665 0.69 -0.3 841,474 + PFLU_RS03665 0.69 -0.8 841,474 + PFLU_RS03665 0.69 -0.4 842,451 + -0.6 842,451 + -0.5 842,451 + +0.6 842,459 + -1.0 842,515 - -1.5 842,548 + +0.6 842,548 + -0.4 842,548 + -1.6 842,548 + -0.7 842,558 + -0.7 842,558 + +0.0 842,558 + +0.4 842,559 - +3.0 842,559 - -0.3 842,575 + -0.4 842,575 + +0.3 842,576 - -3.6 842,576 - -1.0 842,577 + +2.0 842,577 + -0.9 842,577 + +0.2 842,577 + +0.0 842,577 + +0.8 842,577 + +0.7 842,577 + -1.4 842,577 + -1.4 842,577 + +0.4 842,577 + -0.4 842,578 - -1.5 842,578 - -1.2 842,578 - -0.2 842,578 - -1.2 842,578 - +0.1 842,578 - -1.8 842,578 - -1.3 842,578 - -2.3 842,578 - -0.3 842,578 - -2.5 842,578 - -0.4 842,578 - -1.7 842,578 - -2.5 842,604 - -1.3 842,644 + -2.1 842,644 + -2.6 842,644 + -2.2 842,644 + -0.4 842,644 + -2.3 842,644 + -1.7 842,645 - -1.2 842,645 - -2.2
Or see this region's nucleotide sequence