Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_25660 and AO353_25665 are separated by 458 nucleotides AO353_25665 and AO353_25670 are separated by 3 nucleotides
AO353_25660: AO353_25660 - ABC transporter substrate-binding protein, at 5,658,478 to 5,659,257
_25660
AO353_25665: AO353_25665 - 3-hydroxy-isobutyryl-CoA hydrolase (EC 3.1.2.4) (from data) , at 5,659,716 to 5,660,822
_25665
AO353_25670: AO353_25670 - isobutyryl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 5,660,826 to 5,661,977
_25670
Position (kb)
5659
5660
5661 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5658.738 kb on - strand, within AO353_25660 at 5658.752 kb on + strand, within AO353_25660 at 5658.818 kb on + strand, within AO353_25660 at 5658.929 kb on + strand, within AO353_25660 at 5658.930 kb on - strand, within AO353_25660 at 5658.930 kb on - strand, within AO353_25660 at 5659.263 kb on + strand at 5659.263 kb on + strand at 5659.263 kb on + strand at 5659.263 kb on + strand at 5659.263 kb on + strand at 5659.263 kb on + strand at 5659.264 kb on - strand at 5659.264 kb on - strand at 5659.264 kb on - strand at 5659.264 kb on - strand at 5659.264 kb on - strand at 5659.264 kb on - strand at 5659.285 kb on + strand at 5659.367 kb on + strand at 5659.367 kb on + strand at 5659.367 kb on + strand at 5659.367 kb on + strand at 5659.368 kb on - strand at 5659.556 kb on - strand at 5659.559 kb on - strand at 5659.591 kb on - strand at 5659.591 kb on - strand at 5659.593 kb on + strand at 5659.620 kb on + strand at 5659.620 kb on + strand at 5659.620 kb on + strand at 5659.620 kb on + strand at 5659.621 kb on - strand at 5659.621 kb on - strand at 5659.621 kb on - strand at 5659.621 kb on - strand at 5659.621 kb on - strand at 5659.621 kb on - strand at 5659.632 kb on - strand at 5659.685 kb on + strand at 5659.686 kb on - strand at 5659.686 kb on - strand at 5659.686 kb on - strand at 5659.697 kb on + strand at 5659.698 kb on - strand at 5659.698 kb on - strand at 5659.698 kb on - strand at 5659.698 kb on - strand at 5659.711 kb on + strand at 5659.711 kb on + strand at 5659.711 kb on + strand at 5660.025 kb on + strand, within AO353_25665 at 5660.026 kb on - strand, within AO353_25665 at 5660.181 kb on + strand, within AO353_25665 at 5660.205 kb on + strand, within AO353_25665 at 5660.205 kb on + strand, within AO353_25665 at 5660.205 kb on + strand, within AO353_25665 at 5660.205 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.241 kb on + strand, within AO353_25665 at 5660.286 kb on + strand, within AO353_25665 at 5660.286 kb on + strand, within AO353_25665 at 5660.287 kb on - strand, within AO353_25665 at 5660.308 kb on - strand, within AO353_25665 at 5660.396 kb on - strand, within AO353_25665 at 5660.414 kb on - strand, within AO353_25665 at 5660.561 kb on - strand, within AO353_25665 at 5660.594 kb on - strand, within AO353_25665 at 5661.015 kb on + strand, within AO353_25670 at 5661.015 kb on + strand, within AO353_25670 at 5661.015 kb on + strand, within AO353_25670 at 5661.015 kb on + strand, within AO353_25670 at 5661.016 kb on - strand, within AO353_25670 at 5661.016 kb on - strand, within AO353_25670 at 5661.016 kb on - strand, within AO353_25670 at 5661.024 kb on + strand, within AO353_25670 at 5661.024 kb on + strand, within AO353_25670 at 5661.024 kb on + strand, within AO353_25670 at 5661.025 kb on - strand, within AO353_25670 at 5661.033 kb on + strand, within AO353_25670 at 5661.129 kb on + strand, within AO353_25670 at 5661.129 kb on + strand, within AO353_25670 at 5661.129 kb on + strand, within AO353_25670 at 5661.129 kb on + strand, within AO353_25670 at 5661.129 kb on + strand, within AO353_25670 at 5661.129 kb on + strand, within AO353_25670 at 5661.130 kb on - strand, within AO353_25670 at 5661.130 kb on - strand, within AO353_25670 at 5661.407 kb on + strand, within AO353_25670 at 5661.407 kb on + strand, within AO353_25670
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 5,658,738 - AO353_25660 0.33 +0.2 5,658,752 + AO353_25660 0.35 +0.7 5,658,818 + AO353_25660 0.44 +0.3 5,658,929 + AO353_25660 0.58 -1.5 5,658,930 - AO353_25660 0.58 +1.3 5,658,930 - AO353_25660 0.58 -1.4 5,659,263 + +1.6 5,659,263 + -0.9 5,659,263 + +2.1 5,659,263 + -1.0 5,659,263 + +1.1 5,659,263 + -0.5 5,659,264 - -1.1 5,659,264 - -2.1 5,659,264 - -1.8 5,659,264 - +0.1 5,659,264 - -0.1 5,659,264 - -1.1 5,659,285 + -1.1 5,659,367 + +2.8 5,659,367 + -0.7 5,659,367 + -1.3 5,659,367 + +2.5 5,659,368 - -0.3 5,659,556 - +2.5 5,659,559 - +0.6 5,659,591 - -0.8 5,659,591 - +0.2 5,659,593 + -0.4 5,659,620 + +1.7 5,659,620 + -1.1 5,659,620 + +1.1 5,659,620 + -0.7 5,659,621 - -0.8 5,659,621 - -1.9 5,659,621 - +0.1 5,659,621 - -0.5 5,659,621 - +0.1 5,659,621 - +1.7 5,659,632 - +0.5 5,659,685 + +1.1 5,659,686 - -1.4 5,659,686 - +0.3 5,659,686 - +1.1 5,659,697 + +1.6 5,659,698 - -0.3 5,659,698 - -1.5 5,659,698 - +0.5 5,659,698 - -0.3 5,659,711 + -0.3 5,659,711 + +1.4 5,659,711 + -0.5 5,660,025 + AO353_25665 0.28 -0.1 5,660,026 - AO353_25665 0.28 +1.1 5,660,181 + AO353_25665 0.42 -0.9 5,660,205 + AO353_25665 0.44 -0.5 5,660,205 + AO353_25665 0.44 -1.1 5,660,205 + AO353_25665 0.44 -1.5 5,660,205 + AO353_25665 0.44 -2.3 5,660,241 + AO353_25665 0.47 +0.8 5,660,241 + AO353_25665 0.47 -0.9 5,660,241 + AO353_25665 0.47 +0.7 5,660,241 + AO353_25665 0.47 -1.6 5,660,241 + AO353_25665 0.47 -1.3 5,660,286 + AO353_25665 0.51 -1.4 5,660,286 + AO353_25665 0.51 -0.9 5,660,287 - AO353_25665 0.52 -2.3 5,660,308 - AO353_25665 0.53 +0.5 5,660,396 - AO353_25665 0.61 -0.3 5,660,414 - AO353_25665 0.63 +0.5 5,660,561 - AO353_25665 0.76 -0.4 5,660,594 - AO353_25665 0.79 +1.1 5,661,015 + AO353_25670 0.16 -0.9 5,661,015 + AO353_25670 0.16 -0.5 5,661,015 + AO353_25670 0.16 +2.8 5,661,015 + AO353_25670 0.16 +1.1 5,661,016 - AO353_25670 0.16 -0.8 5,661,016 - AO353_25670 0.16 +2.1 5,661,016 - AO353_25670 0.16 -2.0 5,661,024 + AO353_25670 0.17 +1.5 5,661,024 + AO353_25670 0.17 +1.7 5,661,024 + AO353_25670 0.17 +1.5 5,661,025 - AO353_25670 0.17 +1.1 5,661,033 + AO353_25670 0.18 +2.1 5,661,129 + AO353_25670 0.26 -0.9 5,661,129 + AO353_25670 0.26 -0.7 5,661,129 + AO353_25670 0.26 -0.1 5,661,129 + AO353_25670 0.26 -1.4 5,661,129 + AO353_25670 0.26 -1.1 5,661,129 + AO353_25670 0.26 -0.5 5,661,130 - AO353_25670 0.26 -0.9 5,661,130 - AO353_25670 0.26 +0.1 5,661,407 + AO353_25670 0.50 +0.6 5,661,407 + AO353_25670 0.50 -1.9
Or see this region's nucleotide sequence