Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_25390

Experiment: Nitrate reduction, replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_25380 and AO353_25385 are separated by 46 nucleotidesAO353_25385 and AO353_25390 overlap by 4 nucleotidesAO353_25390 and AO353_25395 are separated by 3 nucleotidesAO353_25395 and AO353_25400 are separated by 43 nucleotides AO353_25380: AO353_25380 - bestrophin, at 5,598,338 to 5,599,237 _25380 AO353_25385: AO353_25385 - molybdenum cofactor biosynthesis protein MoaC, at 5,599,284 to 5,599,790 _25385 AO353_25390: AO353_25390 - molybdenum cofactor biosynthesis protein MoaD, at 5,599,787 to 5,600,038 _25390 AO353_25395: AO353_25395 - molybdenum cofactor biosynthesis protein MoaE, at 5,600,042 to 5,600,506 _25395 AO353_25400: AO353_25400 - molybdenum cofactor biosynthesis protein MoaA, at 5,600,550 to 5,601,755 _25400 Position (kb) 5599 5600 5601Strain fitness (log2 ratio) -2 -1 0 1 2at 5598.980 kb on + strand, within AO353_25380at 5598.981 kb on - strand, within AO353_25380at 5598.981 kb on - strand, within AO353_25380at 5598.984 kb on + strand, within AO353_25380at 5598.985 kb on - strand, within AO353_25380at 5598.985 kb on - strand, within AO353_25380at 5599.062 kb on - strand, within AO353_25380at 5599.062 kb on - strand, within AO353_25380at 5599.062 kb on - strand, within AO353_25380at 5599.063 kb on + strand, within AO353_25380at 5599.063 kb on + strand, within AO353_25380at 5599.063 kb on + strand, within AO353_25380at 5599.063 kb on + strand, within AO353_25380at 5599.063 kb on + strand, within AO353_25380at 5599.063 kb on + strand, within AO353_25380at 5599.064 kb on - strand, within AO353_25380at 5599.064 kb on - strand, within AO353_25380at 5599.064 kb on - strand, within AO353_25380at 5599.064 kb on - strand, within AO353_25380at 5599.280 kb on - strandat 5599.280 kb on - strandat 5599.437 kb on - strand, within AO353_25385at 5599.484 kb on + strand, within AO353_25385at 5599.683 kb on + strand, within AO353_25385at 5599.683 kb on + strand, within AO353_25385at 5599.684 kb on - strand, within AO353_25385at 5599.684 kb on - strand, within AO353_25385at 5599.802 kb on + strandat 5599.802 kb on + strandat 5599.802 kb on + strandat 5599.802 kb on + strandat 5599.802 kb on + strandat 5599.802 kb on + strandat 5599.803 kb on - strandat 5599.803 kb on - strandat 5599.972 kb on + strand, within AO353_25390at 5599.973 kb on - strand, within AO353_25390at 5600.067 kb on + strandat 5600.068 kb on - strandat 5600.068 kb on - strandat 5600.068 kb on - strandat 5600.384 kb on + strand, within AO353_25395at 5600.384 kb on + strand, within AO353_25395at 5600.384 kb on + strand, within AO353_25395at 5600.526 kb on + strandat 5600.526 kb on + strandat 5600.527 kb on - strandat 5600.542 kb on + strandat 5600.542 kb on + strandat 5600.543 kb on - strandat 5600.543 kb on - strandat 5600.543 kb on - strandat 5600.552 kb on - strandat 5600.552 kb on - strandat 5600.574 kb on - strandat 5600.716 kb on + strand, within AO353_25400at 5600.717 kb on - strand, within AO353_25400at 5600.717 kb on - strand, within AO353_25400at 5600.717 kb on - strand, within AO353_25400at 5600.717 kb on - strand, within AO353_25400at 5600.812 kb on + strand, within AO353_25400at 5600.813 kb on - strand, within AO353_25400

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B
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5,598,980 + AO353_25380 0.71 -0.3
5,598,981 - AO353_25380 0.71 -2.2
5,598,981 - AO353_25380 0.71 -0.8
5,598,984 + AO353_25380 0.72 -0.3
5,598,985 - AO353_25380 0.72 -0.3
5,598,985 - AO353_25380 0.72 -1.3
5,599,062 - AO353_25380 0.80 -1.0
5,599,062 - AO353_25380 0.80 +2.0
5,599,062 - AO353_25380 0.80 -0.3
5,599,063 + AO353_25380 0.81 -0.8
5,599,063 + AO353_25380 0.81 -0.3
5,599,063 + AO353_25380 0.81 -1.9
5,599,063 + AO353_25380 0.81 -0.5
5,599,063 + AO353_25380 0.81 +1.3
5,599,063 + AO353_25380 0.81 -1.0
5,599,064 - AO353_25380 0.81 -0.3
5,599,064 - AO353_25380 0.81 -1.4
5,599,064 - AO353_25380 0.81 -0.3
5,599,064 - AO353_25380 0.81 -0.3
5,599,280 - -0.5
5,599,280 - -0.3
5,599,437 - AO353_25385 0.30 +0.0
5,599,484 + AO353_25385 0.39 -2.0
5,599,683 + AO353_25385 0.79 -0.9
5,599,683 + AO353_25385 0.79 +0.0
5,599,684 - AO353_25385 0.79 -1.3
5,599,684 - AO353_25385 0.79 -1.3
5,599,802 + +0.0
5,599,802 + -0.5
5,599,802 + +0.5
5,599,802 + -0.3
5,599,802 + -0.8
5,599,802 + -1.1
5,599,803 - -0.8
5,599,803 - -1.4
5,599,972 + AO353_25390 0.73 +0.6
5,599,973 - AO353_25390 0.74 +0.6
5,600,067 + -0.3
5,600,068 - -1.3
5,600,068 - -0.5
5,600,068 - +0.2
5,600,384 + AO353_25395 0.74 +0.2
5,600,384 + AO353_25395 0.74 +0.5
5,600,384 + AO353_25395 0.74 +0.1
5,600,526 + +0.2
5,600,526 + -0.3
5,600,527 - -0.3
5,600,542 + -2.1
5,600,542 + -0.3
5,600,543 - +1.2
5,600,543 - -1.2
5,600,543 - -1.4
5,600,552 - -1.0
5,600,552 - +1.5
5,600,574 - -0.4
5,600,716 + AO353_25400 0.14 +2.4
5,600,717 - AO353_25400 0.14 -0.6
5,600,717 - AO353_25400 0.14 -0.4
5,600,717 - AO353_25400 0.14 +0.4
5,600,717 - AO353_25400 0.14 +0.5
5,600,812 + AO353_25400 0.22 -0.0
5,600,813 - AO353_25400 0.22 +0.2

Or see this region's nucleotide sequence