Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_25325 and AO353_25330 overlap by 4 nucleotides AO353_25330 and AO353_25335 overlap by 8 nucleotides AO353_25335 and AO353_25340 overlap by 1 nucleotides AO353_25340 and AO353_25345 overlap by 1 nucleotides
AO353_25325: AO353_25325 - biotin-independent malonate decarboxylase subunit gamma, at 5,586,035 to 5,586,838
_25325
AO353_25330: AO353_25330 - biotin-independent malonate decarboxylase subunit beta, at 5,586,835 to 5,587,686
_25330
AO353_25335: AO353_25335 - malonate decarboxylase acyl carrier protein, at 5,587,679 to 5,587,978
_25335
AO353_25340: AO353_25340 - triphosphoribosyl-dephospho-CoA synthase MdcB, at 5,587,978 to 5,588,853
_25340
AO353_25345: AO353_25345 - malonate decarboxylase subunit alpha, at 5,588,853 to 5,590,523
_25345
Position (kb) 5587
5588 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5586.829 kb on - strand at 5586.829 kb on - strand at 5587.016 kb on + strand, within AO353_25330 at 5587.016 kb on + strand, within AO353_25330 at 5587.138 kb on - strand, within AO353_25330 at 5587.168 kb on + strand at 5587.168 kb on + strand, within AO353_25330 at 5587.168 kb on + strand, within AO353_25330 at 5587.168 kb on + strand, within AO353_25330 at 5587.169 kb on - strand, within AO353_25330 at 5587.172 kb on + strand at 5587.172 kb on + strand, within AO353_25330 at 5587.173 kb on - strand, within AO353_25330 at 5587.173 kb on - strand, within AO353_25330 at 5587.250 kb on + strand, within AO353_25330 at 5587.251 kb on - strand, within AO353_25330 at 5587.756 kb on + strand, within AO353_25335 at 5587.889 kb on + strand, within AO353_25335 at 5587.889 kb on + strand, within AO353_25335 at 5587.968 kb on - strand at 5588.083 kb on + strand, within AO353_25340 at 5588.083 kb on + strand, within AO353_25340 at 5588.083 kb on + strand, within AO353_25340 at 5588.083 kb on + strand, within AO353_25340 at 5588.336 kb on - strand, within AO353_25340 at 5588.370 kb on - strand, within AO353_25340 at 5588.381 kb on - strand, within AO353_25340 at 5588.637 kb on + strand, within AO353_25340 at 5588.638 kb on - strand, within AO353_25340 at 5588.638 kb on - strand, within AO353_25340 at 5588.638 kb on - strand, within AO353_25340 at 5588.788 kb on + strand at 5588.841 kb on + strand at 5588.841 kb on + strand at 5588.842 kb on - strand at 5588.842 kb on - strand at 5588.855 kb on - strand at 5588.884 kb on + strand at 5588.884 kb on + strand at 5588.884 kb on + strand at 5588.884 kb on + strand at 5588.884 kb on + strand at 5588.885 kb on - strand at 5588.885 kb on - strand at 5588.885 kb on - strand at 5588.885 kb on - strand at 5588.885 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 5,586,829 - +2.0 5,586,829 - -0.3 5,587,016 + AO353_25330 0.21 -2.2 5,587,016 + AO353_25330 0.21 -1.0 5,587,138 - AO353_25330 0.36 -0.8 5,587,168 + +1.0 5,587,168 + AO353_25330 0.39 +1.0 5,587,168 + AO353_25330 0.39 -0.8 5,587,168 + AO353_25330 0.39 -3.0 5,587,169 - AO353_25330 0.39 -1.5 5,587,172 + +1.0 5,587,172 + AO353_25330 0.40 -0.4 5,587,173 - AO353_25330 0.40 +1.7 5,587,173 - AO353_25330 0.40 +0.9 5,587,250 + AO353_25330 0.49 -2.0 5,587,251 - AO353_25330 0.49 +0.2 5,587,756 + AO353_25335 0.26 -1.1 5,587,889 + AO353_25335 0.70 -0.8 5,587,889 + AO353_25335 0.70 -0.9 5,587,968 - -0.5 5,588,083 + AO353_25340 0.12 -1.7 5,588,083 + AO353_25340 0.12 +0.2 5,588,083 + AO353_25340 0.12 +1.0 5,588,083 + AO353_25340 0.12 -1.0 5,588,336 - AO353_25340 0.41 +0.2 5,588,370 - AO353_25340 0.45 -1.0 5,588,381 - AO353_25340 0.46 -1.0 5,588,637 + AO353_25340 0.75 +0.5 5,588,638 - AO353_25340 0.75 +0.7 5,588,638 - AO353_25340 0.75 -0.3 5,588,638 - AO353_25340 0.75 -0.9 5,588,788 + -0.1 5,588,841 + +0.2 5,588,841 + +2.0 5,588,842 - -1.2 5,588,842 - +2.0 5,588,855 - +0.7 5,588,884 + -1.5 5,588,884 + -0.8 5,588,884 + -0.9 5,588,884 + +2.0 5,588,884 + -0.4 5,588,885 - -1.5 5,588,885 - -0.3 5,588,885 - -0.9 5,588,885 - +0.0 5,588,885 - -0.5
Or see this region's nucleotide sequence