Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_24515

Experiment: Nitrate reduction, replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_24505 and AO353_24510 overlap by 4 nucleotidesAO353_24510 and AO353_24515 are separated by 17 nucleotidesAO353_24515 and AO353_24520 are separated by 97 nucleotides AO353_24505: AO353_24505 - transketolase, at 5,387,885 to 5,388,907 _24505 AO353_24510: AO353_24510 - transketolase, at 5,388,904 to 5,389,752 _24510 AO353_24515: AO353_24515 - MFS transporter permease, at 5,389,770 to 5,391,068 _24515 AO353_24520: AO353_24520 - short-chain dehydrogenase, at 5,391,166 to 5,391,915 _24520 Position (kb) 5389 5390 5391 5392Strain fitness (log2 ratio) -2 -1 0 1 2at 5389.310 kb on + strand, within AO353_24510at 5389.310 kb on + strand, within AO353_24510at 5389.310 kb on + strand, within AO353_24510at 5389.310 kb on + strand, within AO353_24510at 5389.415 kb on + strand, within AO353_24510at 5389.416 kb on - strand, within AO353_24510at 5389.461 kb on - strand, within AO353_24510at 5389.461 kb on - strand, within AO353_24510at 5389.461 kb on - strand, within AO353_24510at 5389.461 kb on - strand, within AO353_24510at 5389.511 kb on + strand, within AO353_24510at 5389.511 kb on + strand, within AO353_24510at 5389.511 kb on + strand, within AO353_24510at 5389.512 kb on - strand, within AO353_24510at 5389.527 kb on - strand, within AO353_24510at 5389.544 kb on + strand, within AO353_24510at 5389.544 kb on + strand, within AO353_24510at 5389.544 kb on + strand, within AO353_24510at 5389.544 kb on + strand, within AO353_24510at 5389.544 kb on + strand, within AO353_24510at 5389.545 kb on - strand, within AO353_24510at 5389.604 kb on + strand, within AO353_24510at 5389.650 kb on - strand, within AO353_24510at 5389.650 kb on - strand, within AO353_24510at 5389.650 kb on - strand, within AO353_24510at 5389.650 kb on - strand, within AO353_24510at 5389.698 kb on + strandat 5389.828 kb on - strandat 5389.843 kb on + strandat 5389.843 kb on + strandat 5389.843 kb on + strandat 5389.843 kb on + strandat 5389.843 kb on + strandat 5389.894 kb on + strandat 5390.055 kb on + strandat 5390.056 kb on - strand, within AO353_24515at 5390.056 kb on - strand, within AO353_24515at 5390.056 kb on - strand, within AO353_24515at 5390.113 kb on - strand, within AO353_24515at 5390.164 kb on + strand, within AO353_24515at 5390.164 kb on + strand, within AO353_24515at 5390.164 kb on + strand, within AO353_24515at 5390.164 kb on + strand, within AO353_24515at 5390.165 kb on - strand, within AO353_24515at 5390.165 kb on - strand, within AO353_24515at 5390.192 kb on - strand, within AO353_24515at 5390.192 kb on - strand, within AO353_24515at 5390.192 kb on - strand, within AO353_24515at 5390.192 kb on - strand, within AO353_24515at 5390.302 kb on + strand, within AO353_24515at 5390.303 kb on - strand, within AO353_24515at 5390.458 kb on + strand, within AO353_24515at 5390.458 kb on + strand, within AO353_24515at 5390.458 kb on + strand, within AO353_24515at 5390.459 kb on - strand, within AO353_24515at 5390.609 kb on + strand, within AO353_24515at 5390.656 kb on + strand, within AO353_24515at 5390.656 kb on + strand, within AO353_24515at 5390.657 kb on - strand, within AO353_24515at 5390.666 kb on - strand, within AO353_24515at 5390.725 kb on + strand, within AO353_24515at 5390.725 kb on + strand, within AO353_24515at 5390.725 kb on + strand, within AO353_24515at 5390.725 kb on + strand, within AO353_24515at 5390.726 kb on - strand, within AO353_24515at 5390.726 kb on - strand, within AO353_24515at 5390.726 kb on - strand, within AO353_24515at 5390.857 kb on + strand, within AO353_24515at 5390.857 kb on + strand, within AO353_24515at 5390.857 kb on + strand, within AO353_24515at 5390.858 kb on - strand, within AO353_24515at 5390.858 kb on - strand, within AO353_24515at 5390.963 kb on - strandat 5390.970 kb on + strandat 5390.970 kb on + strandat 5390.970 kb on + strandat 5390.970 kb on + strandat 5390.970 kb on + strandat 5390.970 kb on + strandat 5390.970 kb on + strandat 5390.971 kb on - strandat 5390.971 kb on - strandat 5390.971 kb on - strandat 5390.974 kb on + strandat 5390.974 kb on + strandat 5390.975 kb on - strandat 5390.975 kb on - strandat 5390.975 kb on - strandat 5391.163 kb on + strandat 5391.163 kb on + strandat 5391.163 kb on + strandat 5391.163 kb on + strandat 5391.163 kb on + strandat 5391.164 kb on - strandat 5391.164 kb on - strandat 5391.215 kb on + strandat 5391.215 kb on + strandat 5391.215 kb on + strandat 5391.215 kb on + strandat 5391.216 kb on - strandat 5391.216 kb on - strandat 5391.216 kb on - strandat 5391.216 kb on - strandat 5391.284 kb on - strand, within AO353_24520at 5391.340 kb on - strand, within AO353_24520at 5391.382 kb on + strand, within AO353_24520at 5391.382 kb on + strand, within AO353_24520at 5391.382 kb on + strand, within AO353_24520at 5391.383 kb on - strandat 5391.383 kb on - strand, within AO353_24520at 5391.452 kb on + strand, within AO353_24520at 5391.452 kb on + strand, within AO353_24520at 5391.452 kb on + strand, within AO353_24520at 5391.453 kb on - strand, within AO353_24520at 5391.592 kb on + strand, within AO353_24520at 5391.592 kb on + strand, within AO353_24520at 5391.840 kb on + strand, within AO353_24520at 5391.840 kb on + strand, within AO353_24520at 5391.840 kb on + strand, within AO353_24520at 5391.840 kb on + strand, within AO353_24520at 5391.939 kb on + strandat 5392.068 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B
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5,389,310 + AO353_24510 0.48 -0.1
5,389,310 + AO353_24510 0.48 +0.6
5,389,310 + AO353_24510 0.48 -0.7
5,389,310 + AO353_24510 0.48 +2.0
5,389,415 + AO353_24510 0.60 -0.7
5,389,416 - AO353_24510 0.60 -0.9
5,389,461 - AO353_24510 0.66 -0.3
5,389,461 - AO353_24510 0.66 +0.1
5,389,461 - AO353_24510 0.66 -0.4
5,389,461 - AO353_24510 0.66 +1.7
5,389,511 + AO353_24510 0.71 -0.9
5,389,511 + AO353_24510 0.71 -1.2
5,389,511 + AO353_24510 0.71 -2.3
5,389,512 - AO353_24510 0.72 -1.2
5,389,527 - AO353_24510 0.73 +2.0
5,389,544 + AO353_24510 0.75 -0.9
5,389,544 + AO353_24510 0.75 +0.3
5,389,544 + AO353_24510 0.75 -0.3
5,389,544 + AO353_24510 0.75 +0.5
5,389,544 + AO353_24510 0.75 +0.0
5,389,545 - AO353_24510 0.76 +1.0
5,389,604 + AO353_24510 0.82 +1.0
5,389,650 - AO353_24510 0.88 -2.4
5,389,650 - AO353_24510 0.88 -1.0
5,389,650 - AO353_24510 0.88 +0.1
5,389,650 - AO353_24510 0.88 -2.0
5,389,698 + +0.4
5,389,828 - +0.2
5,389,843 + -0.1
5,389,843 + -0.6
5,389,843 + -1.1
5,389,843 + +1.0
5,389,843 + +0.7
5,389,894 + -1.3
5,390,055 + +0.4
5,390,056 - AO353_24515 0.22 -0.4
5,390,056 - AO353_24515 0.22 -0.4
5,390,056 - AO353_24515 0.22 +0.3
5,390,113 - AO353_24515 0.26 +0.7
5,390,164 + AO353_24515 0.30 +0.7
5,390,164 + AO353_24515 0.30 +0.0
5,390,164 + AO353_24515 0.30 +0.8
5,390,164 + AO353_24515 0.30 +1.3
5,390,165 - AO353_24515 0.30 +0.1
5,390,165 - AO353_24515 0.30 -0.2
5,390,192 - AO353_24515 0.32 +0.4
5,390,192 - AO353_24515 0.32 +0.6
5,390,192 - AO353_24515 0.32 +0.3
5,390,192 - AO353_24515 0.32 +0.4
5,390,302 + AO353_24515 0.41 +0.4
5,390,303 - AO353_24515 0.41 -1.7
5,390,458 + AO353_24515 0.53 -0.4
5,390,458 + AO353_24515 0.53 -0.3
5,390,458 + AO353_24515 0.53 -1.0
5,390,459 - AO353_24515 0.53 -1.4
5,390,609 + AO353_24515 0.65 -1.6
5,390,656 + AO353_24515 0.68 -1.2
5,390,656 + AO353_24515 0.68 -0.8
5,390,657 - AO353_24515 0.68 +1.3
5,390,666 - AO353_24515 0.69 -0.4
5,390,725 + AO353_24515 0.74 +1.0
5,390,725 + AO353_24515 0.74 -0.9
5,390,725 + AO353_24515 0.74 -0.7
5,390,725 + AO353_24515 0.74 -0.3
5,390,726 - AO353_24515 0.74 -1.0
5,390,726 - AO353_24515 0.74 -0.2
5,390,726 - AO353_24515 0.74 +2.6
5,390,857 + AO353_24515 0.84 +0.4
5,390,857 + AO353_24515 0.84 -0.2
5,390,857 + AO353_24515 0.84 -0.3
5,390,858 - AO353_24515 0.84 +0.2
5,390,858 - AO353_24515 0.84 -0.6
5,390,963 - +1.3
5,390,970 + -0.6
5,390,970 + -1.2
5,390,970 + +0.7
5,390,970 + -0.3
5,390,970 + -0.6
5,390,970 + +0.8
5,390,970 + +1.0
5,390,971 - +0.6
5,390,971 - +0.4
5,390,971 - -0.3
5,390,974 + -0.8
5,390,974 + -1.0
5,390,975 - +0.0
5,390,975 - +1.4
5,390,975 - +0.1
5,391,163 + -1.7
5,391,163 + +0.4
5,391,163 + +1.4
5,391,163 + -0.9
5,391,163 + -1.4
5,391,164 - +1.0
5,391,164 - -0.2
5,391,215 + +1.5
5,391,215 + +1.3
5,391,215 + -1.2
5,391,215 + -1.6
5,391,216 - +0.4
5,391,216 - +0.1
5,391,216 - -1.3
5,391,216 - -0.4
5,391,284 - AO353_24520 0.16 -2.7
5,391,340 - AO353_24520 0.23 -0.6
5,391,382 + AO353_24520 0.29 +0.1
5,391,382 + AO353_24520 0.29 -2.0
5,391,382 + AO353_24520 0.29 -1.2
5,391,383 - +1.0
5,391,383 - AO353_24520 0.29 +0.7
5,391,452 + AO353_24520 0.38 -0.3
5,391,452 + AO353_24520 0.38 +1.0
5,391,452 + AO353_24520 0.38 +0.0
5,391,453 - AO353_24520 0.38 -2.4
5,391,592 + AO353_24520 0.57 -2.1
5,391,592 + AO353_24520 0.57 +0.3
5,391,840 + AO353_24520 0.90 -0.6
5,391,840 + AO353_24520 0.90 +0.1
5,391,840 + AO353_24520 0.90 -0.2
5,391,840 + AO353_24520 0.90 +0.0
5,391,939 + -0.2
5,392,068 - +0.6

Or see this region's nucleotide sequence