Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_22695

Experiment: Nitrate reduction, replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_22690 and AO353_22695 are separated by 104 nucleotidesAO353_22695 and AO353_22700 are separated by 47 nucleotidesAO353_22700 and AO353_22705 overlap by 1 nucleotides AO353_22690: AO353_22690 - amidohydrolase, at 4,967,853 to 4,969,598 _22690 AO353_22695: AO353_22695 - LysR family transcriptional regulator, at 4,969,703 to 4,970,620 _22695 AO353_22700: AO353_22700 - LexA family transcriptional regulator, at 4,970,668 to 4,971,285 _22700 AO353_22705: AO353_22705 - CDP-6-deoxy-delta-3,4-glucoseen reductase, at 4,971,285 to 4,971,902 _22705 Position (kb) 4969 4970 4971Strain fitness (log2 ratio) -2 -1 0 1 2at 4968.760 kb on + strand, within AO353_22690at 4968.760 kb on + strand, within AO353_22690at 4968.761 kb on - strand, within AO353_22690at 4968.761 kb on - strand, within AO353_22690at 4968.761 kb on - strand, within AO353_22690at 4968.761 kb on - strand, within AO353_22690at 4968.761 kb on - strand, within AO353_22690at 4968.761 kb on - strandat 4969.177 kb on + strand, within AO353_22690at 4969.178 kb on - strand, within AO353_22690at 4969.178 kb on - strand, within AO353_22690at 4969.178 kb on - strand, within AO353_22690at 4969.178 kb on - strand, within AO353_22690at 4969.368 kb on - strand, within AO353_22690at 4969.592 kb on + strandat 4969.593 kb on - strandat 4969.636 kb on + strandat 4969.872 kb on + strand, within AO353_22695at 4969.872 kb on + strand, within AO353_22695at 4969.872 kb on + strand, within AO353_22695at 4969.895 kb on + strand, within AO353_22695at 4969.895 kb on + strand, within AO353_22695at 4970.214 kb on - strand, within AO353_22695at 4970.276 kb on + strand, within AO353_22695at 4970.277 kb on - strand, within AO353_22695at 4970.277 kb on - strand, within AO353_22695at 4970.277 kb on - strand, within AO353_22695at 4970.277 kb on - strand, within AO353_22695at 4970.277 kb on - strand, within AO353_22695at 4970.378 kb on + strand, within AO353_22695at 4970.379 kb on - strand, within AO353_22695at 4970.379 kb on - strand, within AO353_22695at 4970.379 kb on - strand, within AO353_22695at 4970.490 kb on - strand, within AO353_22695at 4971.019 kb on + strand, within AO353_22700at 4971.208 kb on + strand, within AO353_22700at 4971.209 kb on - strand, within AO353_22700at 4971.209 kb on - strand, within AO353_22700at 4971.543 kb on + strand, within AO353_22705at 4971.543 kb on + strand, within AO353_22705at 4971.543 kb on + strand, within AO353_22705at 4971.543 kb on + strand, within AO353_22705at 4971.543 kb on + strand, within AO353_22705at 4971.543 kb on + strand, within AO353_22705at 4971.543 kb on + strand, within AO353_22705at 4971.544 kb on - strand, within AO353_22705

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B
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4,968,760 + AO353_22690 0.52 +0.0
4,968,760 + AO353_22690 0.52 -2.5
4,968,761 - AO353_22690 0.52 +0.1
4,968,761 - AO353_22690 0.52 -0.5
4,968,761 - AO353_22690 0.52 -2.3
4,968,761 - AO353_22690 0.52 -2.0
4,968,761 - AO353_22690 0.52 +0.2
4,968,761 - +0.1
4,969,177 + AO353_22690 0.76 -0.9
4,969,178 - AO353_22690 0.76 +1.1
4,969,178 - AO353_22690 0.76 +0.1
4,969,178 - AO353_22690 0.76 -1.3
4,969,178 - AO353_22690 0.76 +2.6
4,969,368 - AO353_22690 0.87 -1.3
4,969,592 + +2.1
4,969,593 - -0.8
4,969,636 + -0.3
4,969,872 + AO353_22695 0.18 -0.9
4,969,872 + AO353_22695 0.18 -0.3
4,969,872 + AO353_22695 0.18 -1.5
4,969,895 + AO353_22695 0.21 -2.1
4,969,895 + AO353_22695 0.21 -0.5
4,970,214 - AO353_22695 0.56 +0.6
4,970,276 + AO353_22695 0.62 -1.2
4,970,277 - AO353_22695 0.63 -1.1
4,970,277 - AO353_22695 0.63 -2.0
4,970,277 - AO353_22695 0.63 +0.6
4,970,277 - AO353_22695 0.63 +1.1
4,970,277 - AO353_22695 0.63 +1.1
4,970,378 + AO353_22695 0.74 -2.0
4,970,379 - AO353_22695 0.74 +0.5
4,970,379 - AO353_22695 0.74 -0.9
4,970,379 - AO353_22695 0.74 +0.5
4,970,490 - AO353_22695 0.86 -1.3
4,971,019 + AO353_22700 0.57 -0.5
4,971,208 + AO353_22700 0.87 -0.9
4,971,209 - AO353_22700 0.88 +0.1
4,971,209 - AO353_22700 0.88 +0.2
4,971,543 + AO353_22705 0.42 +0.1
4,971,543 + AO353_22705 0.42 -0.5
4,971,543 + AO353_22705 0.42 +0.1
4,971,543 + AO353_22705 0.42 -0.5
4,971,543 + AO353_22705 0.42 -1.5
4,971,543 + AO353_22705 0.42 -1.6
4,971,543 + AO353_22705 0.42 -0.8
4,971,544 - AO353_22705 0.42 +1.6

Or see this region's nucleotide sequence