Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_22125 and AO353_22130 are separated by 32 nucleotides AO353_22130 and AO353_22135 overlap by 4 nucleotides AO353_22135 and AO353_22140 are separated by 16 nucleotides
AO353_22125: AO353_22125 - anaerobic ribonucleoside-triphosphate reductase, at 4,847,641 to 4,849,656
_22125
AO353_22130: AO353_22130 - oxidoreductase, at 4,849,689 to 4,849,856
_22130
AO353_22135: AO353_22135 - anaerobic ribonucleoside-triphosphate reductase activating protein, at 4,849,853 to 4,850,548
_22135
AO353_22140: AO353_22140 - hypothetical protein, at 4,850,565 to 4,851,944
_22140
Position (kb)
4849
4850 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4848.739 kb on + strand, within AO353_22125 at 4848.815 kb on + strand, within AO353_22125 at 4848.815 kb on + strand, within AO353_22125 at 4848.815 kb on + strand, within AO353_22125 at 4848.815 kb on + strand, within AO353_22125 at 4848.816 kb on - strand, within AO353_22125 at 4848.816 kb on - strand, within AO353_22125 at 4848.882 kb on - strand, within AO353_22125 at 4848.882 kb on - strand, within AO353_22125 at 4848.892 kb on - strand, within AO353_22125 at 4848.892 kb on - strand, within AO353_22125 at 4848.910 kb on + strand, within AO353_22125 at 4848.910 kb on + strand, within AO353_22125 at 4848.910 kb on + strand, within AO353_22125 at 4848.910 kb on + strand, within AO353_22125 at 4848.911 kb on - strand, within AO353_22125 at 4849.031 kb on - strand, within AO353_22125 at 4849.231 kb on + strand, within AO353_22125 at 4849.231 kb on + strand, within AO353_22125 at 4849.231 kb on + strand, within AO353_22125 at 4849.232 kb on - strand, within AO353_22125 at 4849.232 kb on - strand, within AO353_22125 at 4849.267 kb on + strand, within AO353_22125 at 4849.336 kb on + strand, within AO353_22125 at 4849.339 kb on + strand, within AO353_22125 at 4849.340 kb on - strand, within AO353_22125 at 4849.423 kb on + strand, within AO353_22125 at 4849.423 kb on + strand, within AO353_22125 at 4849.424 kb on - strand, within AO353_22125 at 4849.424 kb on - strand, within AO353_22125 at 4849.424 kb on - strand, within AO353_22125 at 4849.450 kb on + strand, within AO353_22125 at 4849.450 kb on + strand, within AO353_22125 at 4849.450 kb on + strand, within AO353_22125 at 4849.450 kb on + strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.451 kb on - strand, within AO353_22125 at 4849.573 kb on + strand at 4849.574 kb on - strand at 4849.574 kb on - strand at 4849.791 kb on - strand, within AO353_22130 at 4849.962 kb on - strand, within AO353_22135 at 4850.300 kb on + strand, within AO353_22135 at 4850.301 kb on - strand, within AO353_22135 at 4850.301 kb on - strand, within AO353_22135 at 4850.301 kb on - strand, within AO353_22135 at 4850.301 kb on - strand, within AO353_22135 at 4850.324 kb on + strand, within AO353_22135 at 4850.325 kb on - strand, within AO353_22135 at 4850.622 kb on + strand at 4850.623 kb on - strand at 4850.623 kb on - strand at 4850.664 kb on - strand at 4850.694 kb on + strand at 4850.721 kb on + strand, within AO353_22140 at 4850.771 kb on - strand, within AO353_22140 at 4850.771 kb on - strand, within AO353_22140 at 4850.771 kb on - strand at 4850.778 kb on + strand, within AO353_22140 at 4850.778 kb on + strand, within AO353_22140 at 4850.778 kb on + strand, within AO353_22140 at 4850.778 kb on + strand, within AO353_22140
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 4,848,739 + AO353_22125 0.54 +1.5 4,848,815 + AO353_22125 0.58 +1.5 4,848,815 + AO353_22125 0.58 -0.9 4,848,815 + AO353_22125 0.58 -1.0 4,848,815 + AO353_22125 0.58 -0.0 4,848,816 - AO353_22125 0.58 +0.5 4,848,816 - AO353_22125 0.58 -0.3 4,848,882 - AO353_22125 0.62 +0.1 4,848,882 - AO353_22125 0.62 +0.1 4,848,892 - AO353_22125 0.62 +1.1 4,848,892 - AO353_22125 0.62 +1.3 4,848,910 + AO353_22125 0.63 +0.1 4,848,910 + AO353_22125 0.63 +2.1 4,848,910 + AO353_22125 0.63 -3.1 4,848,910 + AO353_22125 0.63 +0.5 4,848,911 - AO353_22125 0.63 -0.9 4,849,031 - AO353_22125 0.69 -0.5 4,849,231 + AO353_22125 0.79 -1.1 4,849,231 + AO353_22125 0.79 +0.1 4,849,231 + AO353_22125 0.79 -0.6 4,849,232 - AO353_22125 0.79 -0.0 4,849,232 - AO353_22125 0.79 -0.9 4,849,267 + AO353_22125 0.81 -2.9 4,849,336 + AO353_22125 0.84 +0.1 4,849,339 + AO353_22125 0.84 -1.6 4,849,340 - AO353_22125 0.84 +3.1 4,849,423 + AO353_22125 0.88 -0.5 4,849,423 + AO353_22125 0.88 +0.5 4,849,424 - AO353_22125 0.88 -1.8 4,849,424 - AO353_22125 0.88 -2.4 4,849,424 - AO353_22125 0.88 -0.1 4,849,450 + AO353_22125 0.90 -0.3 4,849,450 + AO353_22125 0.90 -1.4 4,849,450 + AO353_22125 0.90 -0.9 4,849,450 + AO353_22125 0.90 -0.9 4,849,451 - AO353_22125 0.90 -1.6 4,849,451 - AO353_22125 0.90 +0.1 4,849,451 - AO353_22125 0.90 +0.2 4,849,451 - AO353_22125 0.90 +0.1 4,849,451 - AO353_22125 0.90 -1.1 4,849,451 - AO353_22125 0.90 -0.7 4,849,451 - AO353_22125 0.90 -0.5 4,849,451 - AO353_22125 0.90 -1.6 4,849,451 - AO353_22125 0.90 +3.7 4,849,451 - AO353_22125 0.90 -1.4 4,849,451 - AO353_22125 0.90 -2.3 4,849,451 - AO353_22125 0.90 +0.5 4,849,451 - AO353_22125 0.90 -0.3 4,849,451 - AO353_22125 0.90 +0.1 4,849,573 + -0.3 4,849,574 - -2.4 4,849,574 - -0.5 4,849,791 - AO353_22130 0.61 -1.1 4,849,962 - AO353_22135 0.16 -0.7 4,850,300 + AO353_22135 0.64 -0.7 4,850,301 - AO353_22135 0.64 +1.1 4,850,301 - AO353_22135 0.64 -0.9 4,850,301 - AO353_22135 0.64 -1.9 4,850,301 - AO353_22135 0.64 +0.1 4,850,324 + AO353_22135 0.68 -1.3 4,850,325 - AO353_22135 0.68 -0.7 4,850,622 + -1.5 4,850,623 - -1.7 4,850,623 - -0.1 4,850,664 - +0.2 4,850,694 + -0.3 4,850,721 + AO353_22140 0.11 -0.0 4,850,771 - AO353_22140 0.15 +1.1 4,850,771 - AO353_22140 0.15 -1.4 4,850,771 - -0.9 4,850,778 + AO353_22140 0.15 +0.3 4,850,778 + AO353_22140 0.15 +1.5 4,850,778 + AO353_22140 0.15 -1.9 4,850,778 + AO353_22140 0.15 -0.7
Or see this region's nucleotide sequence