Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_20690 and AO353_20695 are separated by 66 nucleotides AO353_20695 and AO353_20700 overlap by 4 nucleotides AO353_20700 and AO353_20705 are separated by 160 nucleotides
AO353_20690: AO353_20690 - amidophosphoribosyltransferase, at 4,539,183 to 4,540,688
_20690
AO353_20695: AO353_20695 - O-succinylhomoserine sulfhydrylase, at 4,540,755 to 4,541,966
_20695
AO353_20700: AO353_20700 - oxidoreductase, at 4,541,963 to 4,542,739
_20700
AO353_20705: AO353_20705 - tRNA-Val, at 4,542,900 to 4,542,975
_20705
Position (kb)
4540
4541
4542 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4540.485 kb on + strand at 4540.677 kb on + strand at 4540.678 kb on - strand at 4540.694 kb on + strand at 4540.695 kb on - strand at 4540.719 kb on + strand at 4540.719 kb on + strand at 4540.719 kb on + strand at 4540.719 kb on + strand at 4540.719 kb on + strand at 4540.719 kb on + strand at 4540.719 kb on + strand at 4540.719 kb on + strand at 4540.720 kb on - strand at 4540.720 kb on - strand at 4540.720 kb on - strand at 4540.720 kb on - strand at 4540.723 kb on + strand at 4540.723 kb on + strand at 4540.723 kb on + strand at 4540.723 kb on + strand at 4540.723 kb on + strand at 4540.723 kb on + strand at 4540.723 kb on + strand at 4540.723 kb on + strand at 4540.723 kb on + strand at 4540.723 kb on + strand at 4540.723 kb on + strand at 4540.724 kb on - strand at 4540.724 kb on - strand at 4540.724 kb on - strand at 4540.724 kb on - strand at 4540.724 kb on - strand at 4540.729 kb on + strand at 4540.804 kb on + strand at 4540.844 kb on + strand at 4540.844 kb on + strand at 4540.845 kb on - strand at 4540.845 kb on - strand at 4540.845 kb on - strand at 4540.845 kb on - strand at 4540.845 kb on - strand at 4540.890 kb on + strand, within AO353_20695 at 4540.891 kb on - strand, within AO353_20695 at 4540.962 kb on + strand, within AO353_20695 at 4541.148 kb on + strand, within AO353_20695 at 4541.149 kb on - strand, within AO353_20695 at 4541.149 kb on - strand, within AO353_20695 at 4541.149 kb on - strand, within AO353_20695 at 4541.178 kb on + strand, within AO353_20695 at 4541.178 kb on + strand, within AO353_20695 at 4541.178 kb on + strand, within AO353_20695 at 4541.178 kb on + strand, within AO353_20695 at 4541.178 kb on + strand, within AO353_20695 at 4541.179 kb on - strand, within AO353_20695 at 4541.179 kb on - strand, within AO353_20695 at 4541.179 kb on - strand, within AO353_20695 at 4541.179 kb on - strand, within AO353_20695 at 4541.179 kb on - strand, within AO353_20695 at 4541.179 kb on - strand, within AO353_20695 at 4541.179 kb on - strand, within AO353_20695 at 4541.816 kb on + strand, within AO353_20695 at 4542.271 kb on + strand, within AO353_20700 at 4542.271 kb on + strand, within AO353_20700 at 4542.271 kb on + strand, within AO353_20700 at 4542.272 kb on - strand, within AO353_20700 at 4542.272 kb on - strand, within AO353_20700 at 4542.362 kb on + strand, within AO353_20700 at 4542.362 kb on + strand, within AO353_20700 at 4542.362 kb on + strand, within AO353_20700 at 4542.363 kb on - strand, within AO353_20700 at 4542.430 kb on + strand, within AO353_20700 at 4542.440 kb on + strand, within AO353_20700 at 4542.542 kb on - strand, within AO353_20700 at 4542.542 kb on - strand, within AO353_20700 at 4542.654 kb on + strand, within AO353_20700 at 4542.655 kb on - strand, within AO353_20700 at 4542.728 kb on + strand at 4542.728 kb on + strand at 4542.729 kb on - strand at 4542.729 kb on - strand at 4542.729 kb on - strand at 4542.729 kb on - strand at 4542.856 kb on + strand at 4542.856 kb on + strand at 4542.857 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 4,540,485 + +1.0 4,540,677 + -0.2 4,540,678 - +0.2 4,540,694 + +3.5 4,540,695 - -1.2 4,540,719 + -0.6 4,540,719 + -1.5 4,540,719 + -2.4 4,540,719 + +1.0 4,540,719 + +0.5 4,540,719 + +0.4 4,540,719 + +0.4 4,540,719 + -0.6 4,540,720 - -0.4 4,540,720 - -0.4 4,540,720 - -0.4 4,540,720 - -2.8 4,540,723 + +2.2 4,540,723 + -2.6 4,540,723 + -0.4 4,540,723 + +0.4 4,540,723 + +0.6 4,540,723 + -0.7 4,540,723 + +0.1 4,540,723 + -1.9 4,540,723 + -0.9 4,540,723 + +0.4 4,540,723 + +0.5 4,540,724 - -0.0 4,540,724 - -0.2 4,540,724 - +0.1 4,540,724 - -2.1 4,540,724 - -0.8 4,540,729 + +1.2 4,540,804 + -1.6 4,540,844 + +0.1 4,540,844 + -0.8 4,540,845 - +0.4 4,540,845 - -1.4 4,540,845 - +0.4 4,540,845 - -0.7 4,540,845 - -0.8 4,540,890 + AO353_20695 0.11 -1.7 4,540,891 - AO353_20695 0.11 +0.4 4,540,962 + AO353_20695 0.17 -1.8 4,541,148 + AO353_20695 0.32 -0.6 4,541,149 - AO353_20695 0.33 +1.4 4,541,149 - AO353_20695 0.33 +0.4 4,541,149 - AO353_20695 0.33 +3.0 4,541,178 + AO353_20695 0.35 -1.6 4,541,178 + AO353_20695 0.35 -0.4 4,541,178 + AO353_20695 0.35 +0.4 4,541,178 + AO353_20695 0.35 -1.2 4,541,178 + AO353_20695 0.35 -2.1 4,541,179 - AO353_20695 0.35 +1.0 4,541,179 - AO353_20695 0.35 -1.7 4,541,179 - AO353_20695 0.35 +0.4 4,541,179 - AO353_20695 0.35 -0.0 4,541,179 - AO353_20695 0.35 -2.9 4,541,179 - AO353_20695 0.35 +1.0 4,541,179 - AO353_20695 0.35 -0.0 4,541,816 + AO353_20695 0.88 -0.8 4,542,271 + AO353_20700 0.40 -0.4 4,542,271 + AO353_20700 0.40 +0.4 4,542,271 + AO353_20700 0.40 +0.8 4,542,272 - AO353_20700 0.40 +1.4 4,542,272 - AO353_20700 0.40 -1.8 4,542,362 + AO353_20700 0.51 -0.2 4,542,362 + AO353_20700 0.51 -0.4 4,542,362 + AO353_20700 0.51 -0.1 4,542,363 - AO353_20700 0.51 -0.5 4,542,430 + AO353_20700 0.60 -2.2 4,542,440 + AO353_20700 0.61 -0.1 4,542,542 - AO353_20700 0.75 +1.4 4,542,542 - AO353_20700 0.75 -0.2 4,542,654 + AO353_20700 0.89 +1.2 4,542,655 - AO353_20700 0.89 +0.6 4,542,728 + -1.2 4,542,728 + +3.0 4,542,729 - -0.8 4,542,729 - -0.8 4,542,729 - +1.0 4,542,729 - +1.2 4,542,856 + -0.0 4,542,856 + +1.0 4,542,857 - -0.0
Or see this region's nucleotide sequence