Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_19755 and AO353_19760 are separated by 97 nucleotides AO353_19760 and AO353_19765 are separated by 94 nucleotides AO353_19765 and AO353_19770 are separated by 153 nucleotides AO353_19770 and AO353_19775 overlap by 1 nucleotides
AO353_19755: AO353_19755 - cytosine deaminase, at 4,343,132 to 4,344,370
_19755
AO353_19760: AO353_19760 - 6-carboxy-5,6,7,8-tetrahydropterin synthase, at 4,344,468 to 4,344,824
_19760
AO353_19765: AO353_19765 - hypothetical protein, at 4,344,919 to 4,345,998
_19765
AO353_19770: AO353_19770 - peptidase, at 4,346,152 to 4,346,466
_19770
AO353_19775: AO353_19775 - transcriptional regulator, at 4,346,466 to 4,347,134
_19775
Position (kb) 4344
4345
4346 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4344.420 kb on + strand at 4344.421 kb on - strand at 4344.421 kb on - strand at 4344.421 kb on - strand at 4344.421 kb on - strand at 4344.426 kb on + strand at 4344.426 kb on + strand at 4344.426 kb on + strand at 4344.469 kb on + strand at 4344.470 kb on - strand at 4344.470 kb on - strand at 4344.481 kb on + strand at 4344.481 kb on + strand at 4344.481 kb on + strand at 4344.481 kb on + strand at 4344.481 kb on + strand at 4344.482 kb on - strand at 4344.482 kb on - strand at 4344.616 kb on + strand, within AO353_19760 at 4344.616 kb on + strand, within AO353_19760 at 4344.616 kb on + strand, within AO353_19760 at 4344.616 kb on + strand, within AO353_19760 at 4344.616 kb on + strand, within AO353_19760 at 4344.617 kb on - strand, within AO353_19760 at 4344.617 kb on - strand, within AO353_19760 at 4344.617 kb on - strand, within AO353_19760 at 4344.803 kb on + strand at 4344.803 kb on + strand at 4344.883 kb on + strand at 4344.920 kb on + strand at 4344.920 kb on + strand at 4344.920 kb on + strand at 4344.921 kb on - strand at 4344.921 kb on - strand at 4345.016 kb on + strand at 4345.017 kb on - strand at 4345.072 kb on + strand, within AO353_19765 at 4345.101 kb on - strand, within AO353_19765 at 4345.101 kb on - strand, within AO353_19765 at 4345.238 kb on + strand, within AO353_19765 at 4345.238 kb on + strand, within AO353_19765 at 4345.256 kb on + strand, within AO353_19765 at 4345.256 kb on + strand, within AO353_19765 at 4345.257 kb on - strand, within AO353_19765 at 4345.257 kb on - strand, within AO353_19765 at 4345.281 kb on - strand, within AO353_19765 at 4345.281 kb on - strand, within AO353_19765 at 4345.312 kb on + strand, within AO353_19765 at 4345.312 kb on + strand, within AO353_19765 at 4345.312 kb on + strand, within AO353_19765 at 4345.312 kb on + strand, within AO353_19765 at 4345.312 kb on + strand, within AO353_19765 at 4345.312 kb on + strand, within AO353_19765 at 4345.313 kb on - strand, within AO353_19765 at 4345.313 kb on - strand, within AO353_19765 at 4345.313 kb on - strand, within AO353_19765 at 4345.313 kb on - strand, within AO353_19765 at 4345.313 kb on - strand, within AO353_19765 at 4345.313 kb on - strand, within AO353_19765 at 4345.706 kb on + strand, within AO353_19765 at 4345.706 kb on + strand, within AO353_19765 at 4345.706 kb on + strand, within AO353_19765 at 4345.706 kb on + strand, within AO353_19765 at 4345.706 kb on + strand, within AO353_19765 at 4345.706 kb on + strand, within AO353_19765 at 4345.707 kb on - strand, within AO353_19765 at 4345.938 kb on + strand at 4345.998 kb on + strand at 4346.027 kb on + strand at 4346.044 kb on + strand at 4346.044 kb on + strand at 4346.044 kb on + strand at 4346.045 kb on - strand at 4346.045 kb on - strand at 4346.078 kb on - strand at 4346.078 kb on - strand at 4346.091 kb on + strand at 4346.091 kb on + strand at 4346.092 kb on - strand at 4346.092 kb on - strand at 4346.092 kb on - strand at 4346.108 kb on + strand at 4346.108 kb on + strand at 4346.113 kb on + strand at 4346.114 kb on - strand at 4346.114 kb on - strand at 4346.114 kb on - strand at 4346.114 kb on - strand at 4346.114 kb on - strand at 4346.114 kb on - strand at 4346.122 kb on + strand at 4346.122 kb on + strand at 4346.123 kb on - strand at 4346.123 kb on - strand at 4346.146 kb on + strand at 4346.147 kb on - strand at 4346.147 kb on - strand at 4346.147 kb on - strand at 4346.150 kb on + strand at 4346.150 kb on + strand at 4346.150 kb on + strand at 4346.150 kb on + strand at 4346.150 kb on + strand at 4346.150 kb on + strand at 4346.150 kb on + strand at 4346.150 kb on + strand at 4346.150 kb on + strand at 4346.150 kb on + strand at 4346.150 kb on + strand at 4346.150 kb on + strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.151 kb on - strand at 4346.161 kb on - strand at 4346.161 kb on - strand at 4346.174 kb on - strand at 4346.174 kb on - strand at 4346.326 kb on - strand, within AO353_19770 at 4346.366 kb on - strand, within AO353_19770 at 4346.366 kb on - strand, within AO353_19770 at 4346.371 kb on + strand, within AO353_19770 at 4346.372 kb on - strand, within AO353_19770 at 4346.372 kb on - strand, within AO353_19770 at 4346.495 kb on - strand at 4346.539 kb on - strand, within AO353_19775 at 4346.578 kb on - strand, within AO353_19775 at 4346.598 kb on + strand, within AO353_19775 at 4346.599 kb on - strand, within AO353_19775 at 4346.599 kb on - strand, within AO353_19775 at 4346.757 kb on + strand, within AO353_19775 at 4346.757 kb on + strand, within AO353_19775 at 4346.757 kb on + strand, within AO353_19775 at 4346.757 kb on + strand, within AO353_19775 at 4346.758 kb on - strand, within AO353_19775 at 4346.946 kb on + strand, within AO353_19775 at 4346.947 kb on - strand, within AO353_19775 at 4346.947 kb on - strand, within AO353_19775
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 4,344,420 + -0.6 4,344,421 - +0.4 4,344,421 - +0.9 4,344,421 - +0.7 4,344,421 - -1.6 4,344,426 + -0.3 4,344,426 + -0.4 4,344,426 + -1.4 4,344,469 + +1.2 4,344,470 - -0.9 4,344,470 - +0.2 4,344,481 + +1.4 4,344,481 + -1.2 4,344,481 + -0.1 4,344,481 + -0.9 4,344,481 + +0.9 4,344,482 - +0.6 4,344,482 - +1.1 4,344,616 + AO353_19760 0.41 -1.2 4,344,616 + AO353_19760 0.41 -0.4 4,344,616 + AO353_19760 0.41 -0.6 4,344,616 + AO353_19760 0.41 +2.9 4,344,616 + AO353_19760 0.41 -0.4 4,344,617 - AO353_19760 0.42 +0.9 4,344,617 - AO353_19760 0.42 +0.9 4,344,617 - AO353_19760 0.42 +0.1 4,344,803 + +2.3 4,344,803 + -0.9 4,344,883 + -1.4 4,344,920 + +0.3 4,344,920 + +0.9 4,344,920 + +0.1 4,344,921 - +4.3 4,344,921 - -0.4 4,345,016 + -0.1 4,345,017 - -0.4 4,345,072 + AO353_19765 0.14 +0.4 4,345,101 - AO353_19765 0.17 -1.1 4,345,101 - AO353_19765 0.17 -0.1 4,345,238 + AO353_19765 0.30 -0.1 4,345,238 + AO353_19765 0.30 -0.9 4,345,256 + AO353_19765 0.31 -0.2 4,345,256 + AO353_19765 0.31 -1.6 4,345,257 - AO353_19765 0.31 +0.8 4,345,257 - AO353_19765 0.31 +1.2 4,345,281 - AO353_19765 0.34 +0.7 4,345,281 - AO353_19765 0.34 -0.4 4,345,312 + AO353_19765 0.36 -0.6 4,345,312 + AO353_19765 0.36 -1.2 4,345,312 + AO353_19765 0.36 +0.9 4,345,312 + AO353_19765 0.36 -2.4 4,345,312 + AO353_19765 0.36 +1.2 4,345,312 + AO353_19765 0.36 +0.3 4,345,313 - AO353_19765 0.36 +2.2 4,345,313 - AO353_19765 0.36 +0.2 4,345,313 - AO353_19765 0.36 -1.5 4,345,313 - AO353_19765 0.36 +0.4 4,345,313 - AO353_19765 0.36 -1.2 4,345,313 - AO353_19765 0.36 -0.4 4,345,706 + AO353_19765 0.73 +0.4 4,345,706 + AO353_19765 0.73 +2.9 4,345,706 + AO353_19765 0.73 +1.4 4,345,706 + AO353_19765 0.73 -1.5 4,345,706 + AO353_19765 0.73 +2.9 4,345,706 + AO353_19765 0.73 +0.6 4,345,707 - AO353_19765 0.73 -1.2 4,345,938 + -0.2 4,345,998 + -0.4 4,346,027 + +0.7 4,346,044 + -0.6 4,346,044 + +1.9 4,346,044 + -1.6 4,346,045 - +1.9 4,346,045 - -0.4 4,346,078 - -0.4 4,346,078 - +1.4 4,346,091 + -1.8 4,346,091 + -0.9 4,346,092 - -0.1 4,346,092 - -0.4 4,346,092 - -1.4 4,346,108 + -0.4 4,346,108 + -1.4 4,346,113 + -1.2 4,346,114 - -0.2 4,346,114 - +1.9 4,346,114 - -0.8 4,346,114 - +0.5 4,346,114 - +0.4 4,346,114 - -2.2 4,346,122 + -1.2 4,346,122 + -2.1 4,346,123 - +0.4 4,346,123 - +0.9 4,346,146 + -0.2 4,346,147 - +0.9 4,346,147 - +1.9 4,346,147 - +0.9 4,346,150 + -1.4 4,346,150 + +1.9 4,346,150 + -1.1 4,346,150 + -0.2 4,346,150 + -1.0 4,346,150 + -1.2 4,346,150 + +1.9 4,346,150 + -0.9 4,346,150 + +0.7 4,346,150 + -0.6 4,346,150 + +1.9 4,346,150 + -0.1 4,346,151 - -0.4 4,346,151 - -0.6 4,346,151 - +0.4 4,346,151 - -0.6 4,346,151 - -1.1 4,346,151 - -1.2 4,346,151 - +1.9 4,346,151 - -1.2 4,346,151 - -0.1 4,346,151 - -1.5 4,346,151 - +1.9 4,346,151 - -1.2 4,346,151 - +0.4 4,346,151 - +0.5 4,346,151 - -1.9 4,346,151 - +2.9 4,346,151 - +0.1 4,346,151 - +0.1 4,346,151 - +0.9 4,346,151 - +0.6 4,346,151 - -1.5 4,346,151 - +0.9 4,346,151 - -2.5 4,346,151 - -1.9 4,346,151 - -0.1 4,346,151 - -1.2 4,346,151 - +1.6 4,346,151 - -1.4 4,346,161 - -0.1 4,346,161 - -1.1 4,346,174 - -1.2 4,346,174 - +0.9 4,346,326 - AO353_19770 0.55 +0.9 4,346,366 - AO353_19770 0.68 +1.2 4,346,366 - AO353_19770 0.68 +0.6 4,346,371 + AO353_19770 0.70 +1.9 4,346,372 - AO353_19770 0.70 +0.6 4,346,372 - AO353_19770 0.70 +1.9 4,346,495 - -0.4 4,346,539 - AO353_19775 0.11 +0.5 4,346,578 - AO353_19775 0.17 +1.4 4,346,598 + AO353_19775 0.20 -1.1 4,346,599 - AO353_19775 0.20 +1.1 4,346,599 - AO353_19775 0.20 -0.1 4,346,757 + AO353_19775 0.43 +0.6 4,346,757 + AO353_19775 0.43 +2.5 4,346,757 + AO353_19775 0.43 -0.6 4,346,757 + AO353_19775 0.43 -1.6 4,346,758 - AO353_19775 0.44 -0.9 4,346,946 + AO353_19775 0.72 -0.1 4,346,947 - AO353_19775 0.72 +1.9 4,346,947 - AO353_19775 0.72 +0.4
Or see this region's nucleotide sequence