Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_15840 and AO353_15845 are separated by 34 nucleotides AO353_15845 and AO353_15850 are separated by 8 nucleotides AO353_15850 and AO353_15855 are separated by 51 nucleotides AO353_15855 and AO353_15860 are separated by 3 nucleotides AO353_15860 and AO353_15865 are separated by 14 nucleotides AO353_15865 and AO353_15870 are separated by 90 nucleotides
AO353_15840: AO353_15840 - scaffolding protein, at 3,498,636 to 3,499,022
_15840
AO353_15845: AO353_15845 - iron-sulfur cluster assembly protein, at 3,499,057 to 3,499,380
_15845
AO353_15850: AO353_15850 - co-chaperone HscB, at 3,499,389 to 3,499,910
_15850
AO353_15855: AO353_15855 - chaperone protein HscA, at 3,499,962 to 3,501,824
_15855
AO353_15860: AO353_15860 - 2Fe-2S ferredoxin, at 3,501,828 to 3,502,169
_15860
AO353_15865: AO353_15865 - Fe-S assembly protein IscX, at 3,502,184 to 3,502,384
_15865
AO353_15870: AO353_15870 - nucleoside diphosphate kinase, at 3,502,475 to 3,502,900
_15870
Position (kb)
3499
3500
3501
3502 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3499.031 kb on + strand at 3499.031 kb on + strand at 3499.044 kb on + strand at 3502.192 kb on + strand at 3502.192 kb on + strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.193 kb on - strand at 3502.259 kb on + strand, within AO353_15865 at 3502.393 kb on + strand at 3502.393 kb on + strand at 3502.393 kb on + strand at 3502.393 kb on + strand at 3502.393 kb on + strand at 3502.393 kb on + strand at 3502.424 kb on + strand at 3502.426 kb on + strand at 3502.426 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 3,499,031 + -0.2 3,499,031 + +0.8 3,499,044 + -1.3 3,502,192 + +1.0 3,502,192 + +1.1 3,502,193 - +0.2 3,502,193 - -0.5 3,502,193 - -0.5 3,502,193 - -0.2 3,502,193 - -2.0 3,502,193 - -1.0 3,502,193 - +0.2 3,502,193 - -1.4 3,502,193 - +1.8 3,502,193 - -0.8 3,502,193 - +0.2 3,502,193 - -2.0 3,502,259 + AO353_15865 0.37 -1.2 3,502,393 + +0.8 3,502,393 + -1.4 3,502,393 + -0.5 3,502,393 + -1.5 3,502,393 + -0.8 3,502,393 + -0.6 3,502,424 + -0.5 3,502,426 + -0.8 3,502,426 + -0.2
Or see this region's nucleotide sequence