Experiment: Nitrate reduction, replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AO353_12575 and AO353_12580 overlap by 4 nucleotides AO353_12580 and AO353_12585 overlap by 4 nucleotides AO353_12585 and AO353_12590 overlap by 4 nucleotides AO353_12590 and AO353_12595 are separated by 10 nucleotides
AO353_12575: AO353_12575 - type II secretion system protein GspJ, at 2,770,671 to 2,771,216
_12575
AO353_12580: AO353_12580 - type II secretory protein PulK, at 2,771,213 to 2,772,067
_12580
AO353_12585: AO353_12585 - hypothetical protein, at 2,772,064 to 2,773,161
_12585
AO353_12590: AO353_12590 - hypothetical protein, at 2,773,158 to 2,773,625
_12590
AO353_12595: AO353_12595 - hypothetical protein, at 2,773,636 to 2,774,913
_12595
Position (kb)
2772
2773
2774 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2771.074 kb on - strand, within AO353_12575 at 2771.074 kb on - strand, within AO353_12575 at 2771.074 kb on - strand, within AO353_12575 at 2771.114 kb on + strand, within AO353_12575 at 2771.115 kb on - strand, within AO353_12575 at 2771.321 kb on - strand, within AO353_12580 at 2771.321 kb on - strand, within AO353_12580 at 2771.455 kb on + strand, within AO353_12580 at 2772.079 kb on + strand at 2772.079 kb on + strand at 2772.079 kb on + strand at 2772.079 kb on + strand at 2772.080 kb on - strand at 2772.147 kb on + strand at 2772.148 kb on - strand at 2772.188 kb on + strand, within AO353_12585 at 2772.188 kb on + strand, within AO353_12585 at 2772.189 kb on - strand, within AO353_12585 at 2772.239 kb on + strand, within AO353_12585 at 2772.239 kb on + strand, within AO353_12585 at 2772.239 kb on + strand, within AO353_12585 at 2772.239 kb on + strand, within AO353_12585 at 2772.239 kb on + strand, within AO353_12585 at 2772.240 kb on - strand, within AO353_12585 at 2772.243 kb on + strand, within AO353_12585 at 2772.243 kb on + strand, within AO353_12585 at 2772.438 kb on + strand, within AO353_12585 at 2772.439 kb on - strand, within AO353_12585 at 2772.490 kb on + strand, within AO353_12585 at 2772.491 kb on - strand, within AO353_12585 at 2772.491 kb on - strand, within AO353_12585 at 2772.839 kb on - strand, within AO353_12585 at 2772.839 kb on - strand, within AO353_12585 at 2772.855 kb on + strand, within AO353_12585 at 2772.855 kb on + strand, within AO353_12585 at 2772.855 kb on + strand, within AO353_12585 at 2772.856 kb on - strand, within AO353_12585 at 2772.856 kb on - strand, within AO353_12585 at 2773.180 kb on + strand at 2773.244 kb on - strand, within AO353_12590 at 2773.244 kb on - strand, within AO353_12590 at 2773.244 kb on - strand, within AO353_12590 at 2773.329 kb on + strand, within AO353_12590 at 2773.329 kb on + strand, within AO353_12590 at 2773.330 kb on - strand, within AO353_12590 at 2773.330 kb on - strand, within AO353_12590 at 2773.330 kb on - strand, within AO353_12590 at 2773.456 kb on + strand, within AO353_12590 at 2773.456 kb on + strand, within AO353_12590 at 2773.457 kb on - strand, within AO353_12590 at 2773.797 kb on - strand, within AO353_12595 at 2773.797 kb on - strand, within AO353_12595 at 2773.797 kb on - strand, within AO353_12595 at 2773.799 kb on + strand, within AO353_12595 at 2773.799 kb on + strand, within AO353_12595 at 2773.799 kb on + strand, within AO353_12595 at 2773.799 kb on + strand, within AO353_12595 at 2773.799 kb on + strand, within AO353_12595 at 2773.800 kb on - strand, within AO353_12595 at 2773.800 kb on - strand, within AO353_12595 at 2773.800 kb on - strand, within AO353_12595 at 2773.800 kb on - strand, within AO353_12595 at 2773.811 kb on + strand, within AO353_12595 at 2773.812 kb on - strand, within AO353_12595 at 2773.812 kb on - strand, within AO353_12595 at 2773.812 kb on - strand, within AO353_12595 at 2774.042 kb on + strand, within AO353_12595 at 2774.042 kb on + strand, within AO353_12595 at 2774.042 kb on + strand, within AO353_12595 at 2774.043 kb on - strand, within AO353_12595 at 2774.043 kb on - strand, within AO353_12595 at 2774.043 kb on - strand, within AO353_12595 at 2774.057 kb on + strand, within AO353_12595 at 2774.057 kb on + strand, within AO353_12595 at 2774.058 kb on - strand, within AO353_12595 at 2774.058 kb on - strand, within AO353_12595 at 2774.058 kb on - strand, within AO353_12595 at 2774.058 kb on - strand, within AO353_12595 at 2774.100 kb on - strand, within AO353_12595 at 2774.121 kb on - strand, within AO353_12595 at 2774.129 kb on + strand, within AO353_12595 at 2774.129 kb on + strand, within AO353_12595 at 2774.129 kb on + strand, within AO353_12595 at 2774.129 kb on + strand, within AO353_12595 at 2774.129 kb on + strand, within AO353_12595 at 2774.129 kb on + strand, within AO353_12595 at 2774.130 kb on - strand, within AO353_12595 at 2774.130 kb on - strand, within AO353_12595 at 2774.130 kb on - strand, within AO353_12595 at 2774.130 kb on - strand, within AO353_12595 at 2774.130 kb on - strand, within AO353_12595
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B remove 2,771,074 - AO353_12575 0.74 +1.8 2,771,074 - AO353_12575 0.74 +0.2 2,771,074 - AO353_12575 0.74 -0.2 2,771,114 + AO353_12575 0.81 +2.2 2,771,115 - AO353_12575 0.81 +0.3 2,771,321 - AO353_12580 0.13 -2.8 2,771,321 - AO353_12580 0.13 +0.5 2,771,455 + AO353_12580 0.28 -1.2 2,772,079 + -1.4 2,772,079 + -0.8 2,772,079 + +3.3 2,772,079 + -1.9 2,772,080 - -1.8 2,772,147 + -0.0 2,772,148 - +1.3 2,772,188 + AO353_12585 0.11 +0.8 2,772,188 + AO353_12585 0.11 +1.8 2,772,189 - AO353_12585 0.11 +1.8 2,772,239 + AO353_12585 0.16 -1.2 2,772,239 + AO353_12585 0.16 +0.0 2,772,239 + AO353_12585 0.16 +0.0 2,772,239 + AO353_12585 0.16 +0.2 2,772,239 + AO353_12585 0.16 -0.9 2,772,240 - AO353_12585 0.16 -0.9 2,772,243 + AO353_12585 0.16 -0.6 2,772,243 + AO353_12585 0.16 -0.6 2,772,438 + AO353_12585 0.34 +0.5 2,772,439 - AO353_12585 0.34 -0.6 2,772,490 + AO353_12585 0.39 -0.6 2,772,491 - AO353_12585 0.39 -0.7 2,772,491 - AO353_12585 0.39 +0.2 2,772,839 - AO353_12585 0.71 -1.2 2,772,839 - AO353_12585 0.71 -1.2 2,772,855 + AO353_12585 0.72 +2.8 2,772,855 + AO353_12585 0.72 -1.0 2,772,855 + AO353_12585 0.72 +0.2 2,772,856 - AO353_12585 0.72 +0.2 2,772,856 - AO353_12585 0.72 +0.2 2,773,180 + -1.8 2,773,244 - AO353_12590 0.18 -1.4 2,773,244 - AO353_12590 0.18 -0.7 2,773,244 - AO353_12590 0.18 +0.5 2,773,329 + AO353_12590 0.37 +0.8 2,773,329 + AO353_12590 0.37 -0.6 2,773,330 - AO353_12590 0.37 -1.2 2,773,330 - AO353_12590 0.37 +0.0 2,773,330 - AO353_12590 0.37 -0.1 2,773,456 + AO353_12590 0.64 +0.8 2,773,456 + AO353_12590 0.64 -0.8 2,773,457 - AO353_12590 0.64 +0.8 2,773,797 - AO353_12595 0.13 -0.8 2,773,797 - AO353_12595 0.13 -1.2 2,773,797 - AO353_12595 0.13 +0.4 2,773,799 + AO353_12595 0.13 -1.2 2,773,799 + AO353_12595 0.13 -0.2 2,773,799 + AO353_12595 0.13 -1.4 2,773,799 + AO353_12595 0.13 -0.2 2,773,799 + AO353_12595 0.13 -2.4 2,773,800 - AO353_12595 0.13 -0.8 2,773,800 - AO353_12595 0.13 -0.9 2,773,800 - AO353_12595 0.13 +0.4 2,773,800 - AO353_12595 0.13 +0.7 2,773,811 + AO353_12595 0.14 +0.2 2,773,812 - AO353_12595 0.14 -0.9 2,773,812 - AO353_12595 0.14 -0.4 2,773,812 - AO353_12595 0.14 -0.6 2,774,042 + AO353_12595 0.32 -2.1 2,774,042 + AO353_12595 0.32 -0.5 2,774,042 + AO353_12595 0.32 +1.4 2,774,043 - AO353_12595 0.32 -0.8 2,774,043 - AO353_12595 0.32 -1.8 2,774,043 - AO353_12595 0.32 +1.0 2,774,057 + AO353_12595 0.33 +0.5 2,774,057 + AO353_12595 0.33 +0.2 2,774,058 - AO353_12595 0.33 +0.8 2,774,058 - AO353_12595 0.33 +1.2 2,774,058 - AO353_12595 0.33 -2.4 2,774,058 - AO353_12595 0.33 -1.7 2,774,100 - AO353_12595 0.36 -2.3 2,774,121 - AO353_12595 0.38 -0.9 2,774,129 + AO353_12595 0.39 +0.2 2,774,129 + AO353_12595 0.39 +0.4 2,774,129 + AO353_12595 0.39 +0.2 2,774,129 + AO353_12595 0.39 -0.8 2,774,129 + AO353_12595 0.39 -1.0 2,774,129 + AO353_12595 0.39 +0.7 2,774,130 - AO353_12595 0.39 -0.0 2,774,130 - AO353_12595 0.39 -0.2 2,774,130 - AO353_12595 0.39 -2.8 2,774,130 - AO353_12595 0.39 +1.4 2,774,130 - AO353_12595 0.39 +2.1
Or see this region's nucleotide sequence