Strain Fitness in Pseudomonas fluorescens FW300-N2E3 around AO353_07375

Experiment: Nitrate reduction, replicate B

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAO353_07370 and AO353_07375 are separated by 199 nucleotidesAO353_07375 and AO353_07380 are separated by 489 nucleotides AO353_07370: AO353_07370 - tRNA threonylcarbamoyl adenosine modification protein TsaD, at 1,618,530 to 1,619,555 _07370 AO353_07375: AO353_07375 - 30S ribosomal protein S21, at 1,619,755 to 1,619,970 _07375 AO353_07380: AO353_07380 - DNA primase, at 1,620,460 to 1,622,421 _07380 Position (kb) 1619 1620Strain fitness (log2 ratio) -2 -1 0 1 2at 1619.599 kb on - strandat 1619.599 kb on - strandat 1620.022 kb on + strandat 1620.022 kb on + strandat 1620.022 kb on + strandat 1620.226 kb on + strandat 1620.226 kb on + strandat 1620.226 kb on + strandat 1620.226 kb on + strandat 1620.226 kb on + strandat 1620.226 kb on + strandat 1620.226 kb on + strandat 1620.226 kb on + strandat 1620.233 kb on + strandat 1620.304 kb on + strandat 1620.304 kb on + strandat 1620.328 kb on + strandat 1620.328 kb on + strandat 1620.328 kb on + strandat 1620.328 kb on + strandat 1620.358 kb on + strandat 1620.358 kb on + strandat 1620.394 kb on + strandat 1620.394 kb on + strandat 1620.394 kb on + strandat 1620.394 kb on + strandat 1620.394 kb on + strandat 1620.394 kb on + strandat 1620.394 kb on + strandat 1620.394 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate B
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1,619,599 - -0.2
1,619,599 - +1.8
1,620,022 + -1.3
1,620,022 + -0.2
1,620,022 + +0.3
1,620,226 + +0.3
1,620,226 + +0.3
1,620,226 + +0.8
1,620,226 + -0.2
1,620,226 + +1.8
1,620,226 + +1.0
1,620,226 + +1.3
1,620,226 + -1.3
1,620,233 + -1.7
1,620,304 + -0.2
1,620,304 + -0.3
1,620,328 + -1.2
1,620,328 + +1.1
1,620,328 + +0.8
1,620,328 + -0.7
1,620,358 + -1.5
1,620,358 + -1.6
1,620,394 + +1.8
1,620,394 + -1.5
1,620,394 + +0.4
1,620,394 + -0.6
1,620,394 + -1.9
1,620,394 + -1.0
1,620,394 + -0.4
1,620,394 + -1.2

Or see this region's nucleotide sequence