Experiment: RCH2 defined with Thallium(I) acetate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_677 and Pf6N2E2_678 are separated by 120 nucleotides Pf6N2E2_678 and Pf6N2E2_679 overlap by 4 nucleotides Pf6N2E2_679 and Pf6N2E2_680 are separated by 191 nucleotides
Pf6N2E2_677 - Transcriptional regulator, AraC family, at 782,169 to 782,918
_677
Pf6N2E2_678 - Putative oxidoreductase in 4-hydroxyproline catabolic gene cluster, at 783,039 to 784,295
_678
Pf6N2E2_679 - Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain, at 784,292 to 784,528
_679
Pf6N2E2_680 - D-amino-acid oxidase (EC 1.4.3.3), at 784,720 to 785,835
_680
Position (kb) 783
784
785 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 782.060 kb on + strand at 782.060 kb on + strand at 782.121 kb on - strand at 782.252 kb on - strand, within Pf6N2E2_677 at 782.456 kb on + strand, within Pf6N2E2_677 at 782.457 kb on - strand, within Pf6N2E2_677 at 782.592 kb on + strand, within Pf6N2E2_677 at 782.592 kb on + strand, within Pf6N2E2_677 at 782.592 kb on + strand, within Pf6N2E2_677 at 782.593 kb on - strand, within Pf6N2E2_677 at 782.593 kb on - strand, within Pf6N2E2_677 at 782.611 kb on + strand, within Pf6N2E2_677 at 782.611 kb on + strand, within Pf6N2E2_677 at 782.914 kb on + strand at 782.914 kb on + strand at 782.914 kb on + strand at 782.914 kb on + strand at 782.914 kb on + strand at 782.914 kb on + strand at 782.915 kb on - strand at 782.915 kb on - strand at 782.915 kb on - strand at 782.915 kb on - strand at 782.915 kb on - strand at 782.915 kb on - strand at 783.240 kb on + strand, within Pf6N2E2_678 at 783.241 kb on - strand, within Pf6N2E2_678 at 783.466 kb on + strand, within Pf6N2E2_678 at 783.466 kb on + strand, within Pf6N2E2_678 at 783.546 kb on + strand, within Pf6N2E2_678 at 783.546 kb on + strand, within Pf6N2E2_678 at 783.622 kb on + strand, within Pf6N2E2_678 at 783.623 kb on - strand, within Pf6N2E2_678 at 784.000 kb on + strand, within Pf6N2E2_678 at 784.001 kb on - strand, within Pf6N2E2_678 at 784.047 kb on - strand, within Pf6N2E2_678 at 784.089 kb on + strand, within Pf6N2E2_678 at 784.089 kb on + strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.387 kb on + strand, within Pf6N2E2_679 at 784.387 kb on + strand, within Pf6N2E2_679 at 784.517 kb on + strand at 784.518 kb on - strand at 784.760 kb on + strand at 784.760 kb on + strand at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.094 kb on + strand, within Pf6N2E2_680 at 785.094 kb on + strand, within Pf6N2E2_680 at 785.095 kb on - strand, within Pf6N2E2_680 at 785.095 kb on - strand, within Pf6N2E2_680 at 785.142 kb on - strand, within Pf6N2E2_680 at 785.142 kb on - strand, within Pf6N2E2_680
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Thallium(I) acetate 0.002 mg/ml remove 782,060 + +0.8 782,060 + -0.8 782,121 - -0.3 782,252 - Pf6N2E2_677 0.11 -0.3 782,456 + Pf6N2E2_677 0.38 -0.0 782,457 - Pf6N2E2_677 0.38 +1.1 782,592 + Pf6N2E2_677 0.56 -0.8 782,592 + Pf6N2E2_677 0.56 -0.2 782,592 + Pf6N2E2_677 0.56 -0.2 782,593 - Pf6N2E2_677 0.57 +0.8 782,593 - Pf6N2E2_677 0.57 +1.1 782,611 + Pf6N2E2_677 0.59 +0.2 782,611 + Pf6N2E2_677 0.59 +0.4 782,914 + -2.2 782,914 + +0.8 782,914 + +0.2 782,914 + -0.6 782,914 + -1.1 782,914 + +1.4 782,915 - +0.4 782,915 - -0.1 782,915 - -1.1 782,915 - -0.3 782,915 - +0.6 782,915 - -0.5 783,240 + Pf6N2E2_678 0.16 +0.9 783,241 - Pf6N2E2_678 0.16 +1.8 783,466 + Pf6N2E2_678 0.34 +0.4 783,466 + Pf6N2E2_678 0.34 +1.8 783,546 + Pf6N2E2_678 0.40 +1.4 783,546 + Pf6N2E2_678 0.40 +0.6 783,622 + Pf6N2E2_678 0.46 -1.6 783,623 - Pf6N2E2_678 0.46 +1.5 784,000 + Pf6N2E2_678 0.76 +1.2 784,001 - Pf6N2E2_678 0.77 +0.7 784,047 - Pf6N2E2_678 0.80 +0.1 784,089 + Pf6N2E2_678 0.84 -0.6 784,089 + Pf6N2E2_678 0.84 +0.4 784,090 - Pf6N2E2_678 0.84 -0.6 784,090 - Pf6N2E2_678 0.84 +1.2 784,090 - Pf6N2E2_678 0.84 -1.3 784,090 - Pf6N2E2_678 0.84 -3.7 784,387 + Pf6N2E2_679 0.40 -1.6 784,387 + Pf6N2E2_679 0.40 +0.4 784,517 + +0.0 784,518 - +1.2 784,760 + +0.8 784,760 + +0.9 785,092 + Pf6N2E2_680 0.33 -0.5 785,092 + Pf6N2E2_680 0.33 +1.3 785,092 + Pf6N2E2_680 0.33 -0.9 785,092 + Pf6N2E2_680 0.33 +2.4 785,092 + Pf6N2E2_680 0.33 +1.4 785,093 - Pf6N2E2_680 0.33 +1.3 785,093 - Pf6N2E2_680 0.33 +1.4 785,093 - Pf6N2E2_680 0.33 +0.3 785,093 - Pf6N2E2_680 0.33 +2.3 785,093 - Pf6N2E2_680 0.33 +0.4 785,093 - Pf6N2E2_680 0.33 +1.1 785,093 - Pf6N2E2_680 0.33 +2.0 785,094 + Pf6N2E2_680 0.34 -1.6 785,094 + Pf6N2E2_680 0.34 -0.6 785,095 - Pf6N2E2_680 0.34 -0.6 785,095 - Pf6N2E2_680 0.34 +0.3 785,142 - Pf6N2E2_680 0.38 +0.9 785,142 - Pf6N2E2_680 0.38 -2.4
Or see this region's nucleotide sequence