Experiment: RCH2 defined with Thallium(I) acetate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_516 and Pf6N2E2_517 are separated by 148 nucleotides Pf6N2E2_517 and Pf6N2E2_518 are separated by 31 nucleotides
Pf6N2E2_516 - Inosose isomerase (EC 5.3.99.-), at 585,558 to 586,379
_516
Pf6N2E2_517 - Epi-inositol hydrolase (EC 3.7.1.-) (from data) , at 586,528 to 588,459
_517
Pf6N2E2_518 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 588,491 to 589,501
_518
Position (kb)
586
587
588
589 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 585.553 kb on + strand at 585.553 kb on + strand at 585.553 kb on + strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.554 kb on - strand at 585.727 kb on + strand, within Pf6N2E2_516 at 585.728 kb on - strand, within Pf6N2E2_516 at 585.829 kb on - strand, within Pf6N2E2_516 at 585.969 kb on + strand, within Pf6N2E2_516 at 585.970 kb on - strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.063 kb on + strand, within Pf6N2E2_516 at 586.064 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.279 kb on - strand, within Pf6N2E2_516 at 586.441 kb on - strand at 586.441 kb on - strand at 586.585 kb on + strand at 586.585 kb on + strand at 586.586 kb on - strand at 586.780 kb on + strand, within Pf6N2E2_517 at 586.781 kb on - strand, within Pf6N2E2_517 at 586.781 kb on - strand, within Pf6N2E2_517 at 586.868 kb on + strand, within Pf6N2E2_517 at 586.869 kb on - strand at 586.869 kb on - strand, within Pf6N2E2_517 at 586.869 kb on - strand, within Pf6N2E2_517 at 586.885 kb on + strand, within Pf6N2E2_517 at 586.982 kb on - strand, within Pf6N2E2_517 at 587.111 kb on - strand, within Pf6N2E2_517 at 587.126 kb on - strand, within Pf6N2E2_517 at 587.183 kb on + strand, within Pf6N2E2_517 at 587.183 kb on + strand, within Pf6N2E2_517 at 587.512 kb on - strand, within Pf6N2E2_517 at 587.675 kb on - strand, within Pf6N2E2_517 at 587.686 kb on + strand, within Pf6N2E2_517 at 587.847 kb on + strand, within Pf6N2E2_517 at 587.895 kb on + strand, within Pf6N2E2_517 at 587.895 kb on + strand, within Pf6N2E2_517 at 587.895 kb on + strand, within Pf6N2E2_517 at 587.895 kb on + strand, within Pf6N2E2_517 at 587.895 kb on + strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.896 kb on - strand, within Pf6N2E2_517 at 587.899 kb on + strand at 587.899 kb on + strand, within Pf6N2E2_517 at 587.899 kb on + strand, within Pf6N2E2_517 at 587.900 kb on - strand, within Pf6N2E2_517 at 587.911 kb on + strand, within Pf6N2E2_517 at 587.912 kb on - strand, within Pf6N2E2_517 at 587.933 kb on - strand, within Pf6N2E2_517 at 587.986 kb on + strand, within Pf6N2E2_517 at 588.010 kb on + strand, within Pf6N2E2_517 at 588.010 kb on + strand, within Pf6N2E2_517 at 588.010 kb on + strand, within Pf6N2E2_517 at 588.010 kb on + strand, within Pf6N2E2_517 at 588.010 kb on + strand, within Pf6N2E2_517 at 588.011 kb on - strand, within Pf6N2E2_517 at 588.011 kb on - strand, within Pf6N2E2_517 at 588.011 kb on - strand, within Pf6N2E2_517 at 588.011 kb on - strand, within Pf6N2E2_517 at 588.011 kb on - strand, within Pf6N2E2_517 at 588.227 kb on - strand, within Pf6N2E2_517 at 588.241 kb on + strand, within Pf6N2E2_517 at 588.242 kb on - strand, within Pf6N2E2_517 at 588.242 kb on - strand, within Pf6N2E2_517 at 588.352 kb on + strand at 588.352 kb on + strand at 588.352 kb on + strand at 588.353 kb on - strand at 588.353 kb on - strand at 588.454 kb on + strand at 588.472 kb on + strand at 588.473 kb on - strand at 588.473 kb on - strand at 588.473 kb on - strand at 588.488 kb on + strand at 588.488 kb on + strand at 588.488 kb on + strand at 588.488 kb on + strand at 588.488 kb on + strand at 588.488 kb on + strand at 588.488 kb on + strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.489 kb on - strand at 588.541 kb on + strand at 588.653 kb on + strand, within Pf6N2E2_518 at 588.876 kb on - strand, within Pf6N2E2_518 at 588.876 kb on - strand, within Pf6N2E2_518 at 588.876 kb on - strand, within Pf6N2E2_518 at 588.981 kb on - strand, within Pf6N2E2_518 at 588.981 kb on - strand, within Pf6N2E2_518 at 588.981 kb on - strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.055 kb on + strand, within Pf6N2E2_518 at 589.056 kb on - strand, within Pf6N2E2_518 at 589.056 kb on - strand, within Pf6N2E2_518 at 589.056 kb on - strand, within Pf6N2E2_518 at 589.188 kb on - strand, within Pf6N2E2_518 at 589.329 kb on - strand, within Pf6N2E2_518 at 589.329 kb on - strand, within Pf6N2E2_518
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Thallium(I) acetate 0.002 mg/ml remove 585,553 + +0.6 585,553 + -0.5 585,553 + -2.3 585,554 - -1.1 585,554 - +0.1 585,554 - +1.1 585,554 - +0.8 585,554 - +2.5 585,554 - +0.5 585,554 - -0.7 585,554 - +0.6 585,727 + Pf6N2E2_516 0.21 +0.1 585,728 - Pf6N2E2_516 0.21 +1.1 585,829 - Pf6N2E2_516 0.33 +0.3 585,969 + Pf6N2E2_516 0.50 +0.5 585,970 - Pf6N2E2_516 0.50 -0.1 586,063 + Pf6N2E2_516 0.61 +0.1 586,063 + Pf6N2E2_516 0.61 +1.0 586,063 + Pf6N2E2_516 0.61 +0.7 586,064 - Pf6N2E2_516 0.62 +1.3 586,279 - Pf6N2E2_516 0.88 +0.1 586,279 - Pf6N2E2_516 0.88 -0.3 586,279 - Pf6N2E2_516 0.88 +0.4 586,441 - +2.1 586,441 - +0.5 586,585 + -0.8 586,585 + -2.7 586,586 - -0.1 586,780 + Pf6N2E2_517 0.13 -0.1 586,781 - Pf6N2E2_517 0.13 +0.8 586,781 - Pf6N2E2_517 0.13 -1.2 586,868 + Pf6N2E2_517 0.18 +0.5 586,869 - +2.1 586,869 - Pf6N2E2_517 0.18 -1.1 586,869 - Pf6N2E2_517 0.18 +0.3 586,885 + Pf6N2E2_517 0.18 -0.5 586,982 - Pf6N2E2_517 0.23 +0.8 587,111 - Pf6N2E2_517 0.30 -0.3 587,126 - Pf6N2E2_517 0.31 -2.5 587,183 + Pf6N2E2_517 0.34 -1.1 587,183 + Pf6N2E2_517 0.34 -0.7 587,512 - Pf6N2E2_517 0.51 -2.1 587,675 - Pf6N2E2_517 0.59 -1.2 587,686 + Pf6N2E2_517 0.60 +0.9 587,847 + Pf6N2E2_517 0.68 -1.5 587,895 + Pf6N2E2_517 0.71 -0.9 587,895 + Pf6N2E2_517 0.71 -1.8 587,895 + Pf6N2E2_517 0.71 +1.0 587,895 + Pf6N2E2_517 0.71 -1.5 587,895 + Pf6N2E2_517 0.71 -0.2 587,896 - Pf6N2E2_517 0.71 -0.0 587,896 - Pf6N2E2_517 0.71 -1.7 587,896 - Pf6N2E2_517 0.71 -1.7 587,896 - Pf6N2E2_517 0.71 -1.8 587,896 - Pf6N2E2_517 0.71 -0.2 587,896 - Pf6N2E2_517 0.71 +0.8 587,896 - Pf6N2E2_517 0.71 -0.2 587,899 + +1.5 587,899 + Pf6N2E2_517 0.71 +1.9 587,899 + Pf6N2E2_517 0.71 -0.7 587,900 - Pf6N2E2_517 0.71 -1.5 587,911 + Pf6N2E2_517 0.72 +0.3 587,912 - Pf6N2E2_517 0.72 -0.7 587,933 - Pf6N2E2_517 0.73 +0.3 587,986 + Pf6N2E2_517 0.75 -0.5 588,010 + Pf6N2E2_517 0.77 -0.8 588,010 + Pf6N2E2_517 0.77 -0.7 588,010 + Pf6N2E2_517 0.77 -0.5 588,010 + Pf6N2E2_517 0.77 -1.1 588,010 + Pf6N2E2_517 0.77 +0.2 588,011 - Pf6N2E2_517 0.77 +1.2 588,011 - Pf6N2E2_517 0.77 +2.8 588,011 - Pf6N2E2_517 0.77 -0.8 588,011 - Pf6N2E2_517 0.77 +0.7 588,011 - Pf6N2E2_517 0.77 -1.1 588,227 - Pf6N2E2_517 0.88 -1.2 588,241 + Pf6N2E2_517 0.89 -0.9 588,242 - Pf6N2E2_517 0.89 +0.1 588,242 - Pf6N2E2_517 0.89 -0.2 588,352 + -0.9 588,352 + -1.5 588,352 + +0.9 588,353 - +2.1 588,353 - -0.1 588,454 + +0.7 588,472 + +0.1 588,473 - -0.5 588,473 - +0.9 588,473 - +1.1 588,488 + -0.2 588,488 + +0.3 588,488 + -0.5 588,488 + -0.2 588,488 + +0.6 588,488 + +0.7 588,488 + +0.5 588,489 - -1.1 588,489 - -0.4 588,489 - -0.9 588,489 - +1.2 588,489 - -0.8 588,489 - -0.1 588,489 - -0.7 588,489 - +0.9 588,489 - +1.1 588,541 + +0.4 588,653 + Pf6N2E2_518 0.16 +0.9 588,876 - Pf6N2E2_518 0.38 +0.8 588,876 - Pf6N2E2_518 0.38 +0.8 588,876 - Pf6N2E2_518 0.38 +0.3 588,981 - Pf6N2E2_518 0.48 -0.2 588,981 - Pf6N2E2_518 0.48 +0.8 588,981 - Pf6N2E2_518 0.48 -1.1 589,055 + Pf6N2E2_518 0.56 -0.2 589,055 + Pf6N2E2_518 0.56 +0.0 589,055 + Pf6N2E2_518 0.56 +1.3 589,055 + Pf6N2E2_518 0.56 +0.3 589,055 + Pf6N2E2_518 0.56 +0.2 589,055 + Pf6N2E2_518 0.56 +0.5 589,055 + Pf6N2E2_518 0.56 +1.3 589,055 + Pf6N2E2_518 0.56 +0.3 589,056 - Pf6N2E2_518 0.56 -0.4 589,056 - Pf6N2E2_518 0.56 +0.4 589,056 - Pf6N2E2_518 0.56 +0.0 589,188 - Pf6N2E2_518 0.69 +1.0 589,329 - Pf6N2E2_518 0.83 +0.5 589,329 - Pf6N2E2_518 0.83 +0.4
Or see this region's nucleotide sequence