Experiment: RCH2 defined with Thallium(I) acetate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_425 and Pf6N2E2_426 are separated by 4 nucleotides Pf6N2E2_426 and Pf6N2E2_427 are separated by 161 nucleotides Pf6N2E2_427 and Pf6N2E2_428 are separated by 125 nucleotides Pf6N2E2_428 and Pf6N2E2_429 are separated by 117 nucleotides
Pf6N2E2_425 - FIG00957304: hypothetical protein, at 494,309 to 494,842
_425
Pf6N2E2_426 - Arsenate reductase (EC 1.20.4.1), at 494,847 to 495,302
_426
Pf6N2E2_427 - Conidiation-specific protein 10, at 495,464 to 495,643
_427
Pf6N2E2_428 - Protease subunit of ATP-dependent Clp proteases, at 495,769 to 496,332
_428
Pf6N2E2_429 - Hypothetical protein PA2244 (similar to DNA topoisomerase IB, but possibly involved in glycosyl-transfer), at 496,450 to 497,505
_429
Position (kb)
495
496
497 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 494.828 kb on + strand at 495.046 kb on + strand, within Pf6N2E2_426 at 495.047 kb on - strand, within Pf6N2E2_426 at 495.047 kb on - strand, within Pf6N2E2_426 at 495.047 kb on - strand, within Pf6N2E2_426 at 495.147 kb on + strand, within Pf6N2E2_426 at 495.147 kb on + strand, within Pf6N2E2_426 at 495.148 kb on - strand, within Pf6N2E2_426 at 495.148 kb on - strand, within Pf6N2E2_426 at 495.148 kb on - strand, within Pf6N2E2_426 at 495.148 kb on - strand, within Pf6N2E2_426 at 495.358 kb on + strand at 495.358 kb on + strand at 495.359 kb on - strand at 495.359 kb on - strand at 495.359 kb on - strand at 495.360 kb on + strand at 495.377 kb on - strand at 495.378 kb on + strand at 495.689 kb on - strand at 495.689 kb on - strand at 495.770 kb on + strand at 495.771 kb on - strand at 495.771 kb on - strand at 495.818 kb on - strand at 495.906 kb on + strand, within Pf6N2E2_428 at 495.944 kb on + strand, within Pf6N2E2_428 at 495.944 kb on + strand, within Pf6N2E2_428 at 495.944 kb on + strand, within Pf6N2E2_428 at 495.990 kb on - strand, within Pf6N2E2_428 at 495.990 kb on - strand, within Pf6N2E2_428 at 496.031 kb on + strand, within Pf6N2E2_428 at 496.032 kb on - strand, within Pf6N2E2_428 at 496.097 kb on + strand, within Pf6N2E2_428 at 496.098 kb on - strand, within Pf6N2E2_428 at 496.098 kb on - strand, within Pf6N2E2_428 at 496.098 kb on - strand, within Pf6N2E2_428 at 496.098 kb on - strand, within Pf6N2E2_428 at 496.098 kb on - strand, within Pf6N2E2_428 at 496.098 kb on - strand, within Pf6N2E2_428 at 496.144 kb on + strand, within Pf6N2E2_428 at 496.145 kb on - strand, within Pf6N2E2_428 at 496.145 kb on - strand, within Pf6N2E2_428 at 496.175 kb on + strand, within Pf6N2E2_428 at 496.175 kb on + strand, within Pf6N2E2_428 at 496.266 kb on + strand, within Pf6N2E2_428 at 496.387 kb on + strand at 496.388 kb on - strand at 496.508 kb on - strand at 496.542 kb on + strand at 496.542 kb on + strand at 496.543 kb on - strand at 496.543 kb on - strand at 496.613 kb on + strand, within Pf6N2E2_429 at 496.613 kb on + strand, within Pf6N2E2_429 at 496.614 kb on - strand, within Pf6N2E2_429 at 496.614 kb on - strand, within Pf6N2E2_429 at 497.027 kb on + strand, within Pf6N2E2_429 at 497.027 kb on + strand, within Pf6N2E2_429 at 497.028 kb on - strand, within Pf6N2E2_429 at 497.028 kb on - strand, within Pf6N2E2_429 at 497.201 kb on + strand, within Pf6N2E2_429 at 497.202 kb on - strand, within Pf6N2E2_429 at 497.202 kb on - strand, within Pf6N2E2_429 at 497.202 kb on - strand, within Pf6N2E2_429 at 497.202 kb on - strand, within Pf6N2E2_429 at 497.202 kb on - strand, within Pf6N2E2_429 at 497.247 kb on - strand, within Pf6N2E2_429 at 497.322 kb on + strand, within Pf6N2E2_429 at 497.324 kb on + strand, within Pf6N2E2_429 at 497.324 kb on + strand, within Pf6N2E2_429 at 497.324 kb on + strand, within Pf6N2E2_429
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Thallium(I) acetate 0.002 mg/ml remove 494,828 + +0.4 495,046 + Pf6N2E2_426 0.44 -0.2 495,047 - Pf6N2E2_426 0.44 +0.3 495,047 - Pf6N2E2_426 0.44 -0.7 495,047 - Pf6N2E2_426 0.44 -0.2 495,147 + Pf6N2E2_426 0.66 +2.9 495,147 + Pf6N2E2_426 0.66 +0.9 495,148 - Pf6N2E2_426 0.66 +0.8 495,148 - Pf6N2E2_426 0.66 +0.3 495,148 - Pf6N2E2_426 0.66 -0.4 495,148 - Pf6N2E2_426 0.66 -0.4 495,358 + -0.3 495,358 + +0.9 495,359 - -0.7 495,359 - -0.4 495,359 - +0.9 495,360 + +1.7 495,377 - +2.4 495,378 + +0.3 495,689 - +0.4 495,689 - -1.9 495,770 + +0.3 495,771 - +0.0 495,771 - +0.4 495,818 - -0.1 495,906 + Pf6N2E2_428 0.24 -2.0 495,944 + Pf6N2E2_428 0.31 -2.0 495,944 + Pf6N2E2_428 0.31 -0.4 495,944 + Pf6N2E2_428 0.31 -0.5 495,990 - Pf6N2E2_428 0.39 -1.7 495,990 - Pf6N2E2_428 0.39 +0.0 496,031 + Pf6N2E2_428 0.46 -0.4 496,032 - Pf6N2E2_428 0.47 -0.6 496,097 + Pf6N2E2_428 0.58 +0.0 496,098 - Pf6N2E2_428 0.58 +0.6 496,098 - Pf6N2E2_428 0.58 -1.9 496,098 - Pf6N2E2_428 0.58 -2.0 496,098 - Pf6N2E2_428 0.58 +0.4 496,098 - Pf6N2E2_428 0.58 -1.7 496,098 - Pf6N2E2_428 0.58 +0.1 496,144 + Pf6N2E2_428 0.66 -2.4 496,145 - Pf6N2E2_428 0.67 -0.9 496,145 - Pf6N2E2_428 0.67 +0.6 496,175 + Pf6N2E2_428 0.72 +1.4 496,175 + Pf6N2E2_428 0.72 +0.9 496,266 + Pf6N2E2_428 0.88 -0.4 496,387 + +0.0 496,388 - -0.4 496,508 - -1.6 496,542 + -0.5 496,542 + +1.3 496,543 - +1.3 496,543 - +0.0 496,613 + Pf6N2E2_429 0.15 +0.5 496,613 + Pf6N2E2_429 0.15 +1.1 496,614 - Pf6N2E2_429 0.16 +0.6 496,614 - Pf6N2E2_429 0.16 +0.6 497,027 + Pf6N2E2_429 0.55 -2.0 497,027 + Pf6N2E2_429 0.55 -1.4 497,028 - Pf6N2E2_429 0.55 +0.7 497,028 - Pf6N2E2_429 0.55 -0.3 497,201 + Pf6N2E2_429 0.71 +1.7 497,202 - Pf6N2E2_429 0.71 +2.2 497,202 - Pf6N2E2_429 0.71 -0.4 497,202 - Pf6N2E2_429 0.71 +1.5 497,202 - Pf6N2E2_429 0.71 -1.0 497,202 - Pf6N2E2_429 0.71 +0.6 497,247 - Pf6N2E2_429 0.75 +0.1 497,322 + Pf6N2E2_429 0.83 -1.7 497,324 + Pf6N2E2_429 0.83 +1.6 497,324 + Pf6N2E2_429 0.83 +0.7 497,324 + Pf6N2E2_429 0.83 -0.4
Or see this region's nucleotide sequence