Experiment: RCH2 defined with Thallium(I) acetate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_402 and Pf6N2E2_403 are separated by 39 nucleotides Pf6N2E2_403 and Pf6N2E2_404 are separated by 244 nucleotides
Pf6N2E2_402 - Carbonic anhydrase (EC 4.2.1.1), at 472,318 to 472,977
_402
Pf6N2E2_403 - Cyanate hydratase (EC 4.2.1.104), at 473,017 to 473,487
_403
Pf6N2E2_404 - Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit, at 473,732 to 473,854
_404
Position (kb) 473
474 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 472.751 kb on + strand, within Pf6N2E2_402 at 472.861 kb on + strand, within Pf6N2E2_402 at 472.861 kb on + strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.862 kb on - strand, within Pf6N2E2_402 at 472.985 kb on - strand at 473.012 kb on - strand at 473.299 kb on + strand, within Pf6N2E2_403 at 473.299 kb on + strand, within Pf6N2E2_403 at 473.299 kb on + strand, within Pf6N2E2_403 at 473.300 kb on - strand, within Pf6N2E2_403 at 473.300 kb on - strand, within Pf6N2E2_403 at 473.309 kb on - strand, within Pf6N2E2_403 at 473.309 kb on - strand, within Pf6N2E2_403 at 473.327 kb on - strand, within Pf6N2E2_403 at 473.327 kb on - strand, within Pf6N2E2_403 at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.464 kb on + strand at 473.465 kb on - strand at 473.465 kb on - strand at 473.465 kb on - strand at 473.472 kb on + strand at 473.473 kb on - strand at 473.490 kb on + strand at 473.491 kb on - strand at 473.491 kb on - strand at 473.491 kb on - strand at 473.491 kb on - strand at 473.523 kb on - strand at 473.523 kb on - strand at 473.540 kb on + strand at 473.540 kb on + strand at 473.586 kb on + strand at 473.586 kb on + strand at 473.587 kb on - strand at 473.587 kb on - strand at 473.587 kb on - strand at 473.587 kb on - strand at 473.620 kb on - strand at 473.620 kb on - strand at 473.620 kb on - strand at 473.665 kb on + strand at 473.665 kb on + strand at 473.666 kb on - strand at 473.684 kb on - strand at 473.755 kb on + strand, within Pf6N2E2_404 at 473.756 kb on - strand, within Pf6N2E2_404 at 473.756 kb on - strand, within Pf6N2E2_404 at 473.756 kb on - strand, within Pf6N2E2_404 at 473.766 kb on + strand, within Pf6N2E2_404 at 473.766 kb on + strand, within Pf6N2E2_404 at 473.766 kb on + strand, within Pf6N2E2_404 at 473.767 kb on - strand, within Pf6N2E2_404 at 473.787 kb on - strand, within Pf6N2E2_404 at 473.787 kb on - strand, within Pf6N2E2_404 at 473.823 kb on - strand, within Pf6N2E2_404 at 473.830 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on + strand, within Pf6N2E2_404 at 473.832 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.833 kb on - strand, within Pf6N2E2_404 at 473.836 kb on + strand, within Pf6N2E2_404 at 473.944 kb on + strand at 473.944 kb on + strand at 473.945 kb on - strand at 473.947 kb on + strand at 473.947 kb on + strand at 473.948 kb on - strand at 473.948 kb on - strand at 473.948 kb on - strand at 473.989 kb on - strand at 473.989 kb on - strand at 473.989 kb on - strand at 473.994 kb on + strand at 473.995 kb on - strand at 474.000 kb on - strand at 474.125 kb on - strand at 474.164 kb on + strand at 474.164 kb on + strand at 474.165 kb on - strand at 474.165 kb on - strand at 474.236 kb on + strand at 474.236 kb on + strand at 474.236 kb on + strand at 474.236 kb on + strand at 474.237 kb on - strand at 474.312 kb on + strand at 474.313 kb on - strand at 474.314 kb on + strand at 474.329 kb on + strand at 474.388 kb on + strand at 474.388 kb on + strand at 474.388 kb on + strand at 474.388 kb on + strand at 474.389 kb on - strand at 474.475 kb on + strand at 474.475 kb on + strand at 474.475 kb on + strand at 474.476 kb on - strand at 474.506 kb on - strand at 474.513 kb on + strand at 474.513 kb on + strand at 474.513 kb on + strand at 474.514 kb on - strand at 474.627 kb on + strand at 474.627 kb on + strand at 474.834 kb on + strand at 474.835 kb on - strand at 474.835 kb on - strand at 474.840 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Thallium(I) acetate 0.002 mg/ml remove 472,751 + Pf6N2E2_402 0.66 +0.1 472,861 + Pf6N2E2_402 0.82 -0.4 472,861 + Pf6N2E2_402 0.82 -2.0 472,862 - Pf6N2E2_402 0.82 +2.2 472,862 - Pf6N2E2_402 0.82 -0.3 472,862 - Pf6N2E2_402 0.82 +0.6 472,862 - Pf6N2E2_402 0.82 +1.9 472,862 - Pf6N2E2_402 0.82 -1.7 472,985 - -0.3 473,012 - +0.5 473,299 + Pf6N2E2_403 0.60 +0.6 473,299 + Pf6N2E2_403 0.60 -1.0 473,299 + Pf6N2E2_403 0.60 +0.6 473,300 - Pf6N2E2_403 0.60 +0.2 473,300 - Pf6N2E2_403 0.60 -2.0 473,309 - Pf6N2E2_403 0.62 +0.2 473,309 - Pf6N2E2_403 0.62 -0.0 473,327 - Pf6N2E2_403 0.66 +0.5 473,327 - Pf6N2E2_403 0.66 -0.4 473,464 + +0.7 473,464 + +0.6 473,464 + +1.1 473,464 + +0.9 473,464 + +0.3 473,464 + +0.8 473,464 + +0.6 473,465 - -0.3 473,465 - -0.4 473,465 - +0.6 473,472 + -0.5 473,473 - -2.6 473,490 + +1.3 473,491 - -0.2 473,491 - +1.1 473,491 - -0.5 473,491 - -3.1 473,523 - -2.2 473,523 - -0.6 473,540 + -1.4 473,540 + -1.7 473,586 + +0.8 473,586 + -0.4 473,587 - -2.0 473,587 - -0.4 473,587 - -2.4 473,587 - -0.4 473,620 - -1.1 473,620 - -0.2 473,620 - +0.9 473,665 + -1.0 473,665 + +1.6 473,666 - +1.3 473,684 - -0.1 473,755 + Pf6N2E2_404 0.19 -0.0 473,756 - Pf6N2E2_404 0.20 -1.4 473,756 - Pf6N2E2_404 0.20 +0.6 473,756 - Pf6N2E2_404 0.20 -1.1 473,766 + Pf6N2E2_404 0.28 -0.6 473,766 + Pf6N2E2_404 0.28 -1.0 473,766 + Pf6N2E2_404 0.28 +0.2 473,767 - Pf6N2E2_404 0.28 -0.7 473,787 - Pf6N2E2_404 0.45 +2.2 473,787 - Pf6N2E2_404 0.45 -1.0 473,823 - Pf6N2E2_404 0.74 +0.1 473,830 + Pf6N2E2_404 0.80 +0.1 473,832 + Pf6N2E2_404 0.81 +0.5 473,832 + Pf6N2E2_404 0.81 -0.0 473,832 + Pf6N2E2_404 0.81 +0.7 473,832 + Pf6N2E2_404 0.81 +0.3 473,832 + Pf6N2E2_404 0.81 -0.7 473,832 + Pf6N2E2_404 0.81 -0.1 473,832 + Pf6N2E2_404 0.81 +0.7 473,832 + Pf6N2E2_404 0.81 +0.4 473,832 + Pf6N2E2_404 0.81 -1.2 473,832 + Pf6N2E2_404 0.81 -0.4 473,832 - Pf6N2E2_404 0.81 +0.1 473,833 - Pf6N2E2_404 0.82 +0.6 473,833 - Pf6N2E2_404 0.82 +0.7 473,833 - Pf6N2E2_404 0.82 -1.3 473,833 - Pf6N2E2_404 0.82 +0.6 473,833 - Pf6N2E2_404 0.82 -0.9 473,836 + Pf6N2E2_404 0.85 -1.4 473,944 + +0.6 473,944 + -0.2 473,945 - +0.4 473,947 + -1.0 473,947 + -1.7 473,948 - -0.3 473,948 - -0.2 473,948 - +1.0 473,989 - -0.2 473,989 - +0.1 473,989 - +0.3 473,994 + +0.3 473,995 - -1.0 474,000 - +1.0 474,125 - -0.8 474,164 + +0.3 474,164 + -0.7 474,165 - -0.4 474,165 - +0.3 474,236 + +0.6 474,236 + -1.7 474,236 + -1.5 474,236 + +1.0 474,237 - -0.4 474,312 + -0.4 474,313 - -1.0 474,314 + +0.6 474,329 + -3.3 474,388 + +1.9 474,388 + -1.0 474,388 + -0.9 474,388 + -0.4 474,389 - -0.3 474,475 + -0.5 474,475 + -0.3 474,475 + -0.3 474,476 - +0.6 474,506 - -0.2 474,513 + +0.1 474,513 + +1.6 474,513 + +0.2 474,514 - +1.3 474,627 + +0.5 474,627 + +0.6 474,834 + +0.6 474,835 - +1.6 474,835 - +0.0 474,840 + -1.4
Or see this region's nucleotide sequence