Experiment: RCH2 defined with Thallium(I) acetate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_349 and Pf6N2E2_350 are separated by 65 nucleotides Pf6N2E2_350 and Pf6N2E2_351 are separated by 5 nucleotides Pf6N2E2_351 and Pf6N2E2_352 are separated by 203 nucleotides
Pf6N2E2_349 - hypothetical protein, at 415,040 to 415,774
_349
Pf6N2E2_350 - Gfa-like protein, at 415,840 to 416,232
_350
Pf6N2E2_351 - Aldehyde dehydrogenase (EC 1.2.1.3), at 416,238 to 417,686
_351
Pf6N2E2_352 - Opine oxidase subunit B, at 417,890 to 419,071
_352
Position (kb) 416
417
418 Strain fitness (log2 ratio) -2
-1
0
1
2 at 415.347 kb on + strand, within Pf6N2E2_349 at 415.515 kb on + strand, within Pf6N2E2_349 at 415.516 kb on - strand, within Pf6N2E2_349 at 415.542 kb on - strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.660 kb on + strand, within Pf6N2E2_349 at 415.772 kb on + strand at 415.773 kb on - strand at 415.806 kb on + strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.807 kb on - strand at 415.865 kb on + strand at 416.131 kb on + strand, within Pf6N2E2_350 at 416.192 kb on - strand, within Pf6N2E2_350 at 416.231 kb on - strand at 416.231 kb on - strand at 416.272 kb on + strand at 416.272 kb on + strand at 416.272 kb on + strand at 416.297 kb on - strand at 416.323 kb on + strand at 416.449 kb on + strand, within Pf6N2E2_351 at 416.584 kb on + strand, within Pf6N2E2_351 at 416.584 kb on + strand, within Pf6N2E2_351 at 416.585 kb on - strand, within Pf6N2E2_351 at 417.212 kb on - strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.301 kb on + strand, within Pf6N2E2_351 at 417.302 kb on - strand, within Pf6N2E2_351 at 417.302 kb on - strand, within Pf6N2E2_351 at 417.330 kb on + strand, within Pf6N2E2_351 at 417.531 kb on + strand, within Pf6N2E2_351 at 417.640 kb on + strand at 417.640 kb on + strand at 417.640 kb on + strand at 417.640 kb on + strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.641 kb on - strand at 417.701 kb on + strand at 417.701 kb on + strand at 417.712 kb on + strand at 417.712 kb on + strand at 417.713 kb on - strand at 417.713 kb on - strand at 417.759 kb on + strand at 417.760 kb on - strand at 417.760 kb on - strand at 417.781 kb on + strand at 417.818 kb on + strand at 417.819 kb on - strand at 417.819 kb on - strand at 418.075 kb on - strand, within Pf6N2E2_352 at 418.075 kb on - strand, within Pf6N2E2_352 at 418.131 kb on + strand, within Pf6N2E2_352 at 418.131 kb on + strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.132 kb on - strand, within Pf6N2E2_352 at 418.317 kb on + strand, within Pf6N2E2_352 at 418.318 kb on - strand, within Pf6N2E2_352 at 418.318 kb on - strand, within Pf6N2E2_352 at 418.335 kb on - strand, within Pf6N2E2_352 at 418.496 kb on + strand, within Pf6N2E2_352 at 418.497 kb on - strand, within Pf6N2E2_352 at 418.653 kb on + strand, within Pf6N2E2_352 at 418.654 kb on - strand, within Pf6N2E2_352
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Thallium(I) acetate 0.002 mg/ml remove 415,347 + Pf6N2E2_349 0.42 -1.5 415,515 + Pf6N2E2_349 0.65 +0.1 415,516 - Pf6N2E2_349 0.65 +2.2 415,542 - Pf6N2E2_349 0.68 +1.8 415,660 + Pf6N2E2_349 0.84 +0.2 415,660 + Pf6N2E2_349 0.84 +0.4 415,772 + +2.0 415,773 - +0.8 415,806 + +0.2 415,807 - -1.4 415,807 - -1.4 415,807 - +0.0 415,807 - -0.2 415,865 + -1.0 416,131 + Pf6N2E2_350 0.74 +0.6 416,192 - Pf6N2E2_350 0.90 +0.6 416,231 - +0.6 416,231 - -0.1 416,272 + -0.7 416,272 + +1.8 416,272 + +0.1 416,297 - +0.2 416,323 + +0.0 416,449 + Pf6N2E2_351 0.15 -1.0 416,584 + Pf6N2E2_351 0.24 -1.0 416,584 + Pf6N2E2_351 0.24 -1.2 416,585 - Pf6N2E2_351 0.24 -0.7 417,212 - Pf6N2E2_351 0.67 -1.0 417,301 + Pf6N2E2_351 0.73 -0.2 417,301 + Pf6N2E2_351 0.73 -1.2 417,301 + Pf6N2E2_351 0.73 -0.4 417,302 - Pf6N2E2_351 0.73 -1.0 417,302 - Pf6N2E2_351 0.73 +0.6 417,330 + Pf6N2E2_351 0.75 +0.7 417,531 + Pf6N2E2_351 0.89 -0.1 417,640 + -0.0 417,640 + +1.2 417,640 + -0.3 417,640 + -1.5 417,641 - +1.4 417,641 - +1.5 417,641 - +1.2 417,641 - -0.7 417,641 - -0.2 417,701 + -0.2 417,701 + -0.7 417,712 + -0.1 417,712 + +0.1 417,713 - +0.0 417,713 - -1.4 417,759 + +1.3 417,760 - -0.4 417,760 - +0.6 417,781 + +1.6 417,818 + +1.2 417,819 - +0.4 417,819 - -0.2 418,075 - Pf6N2E2_352 0.16 -1.0 418,075 - Pf6N2E2_352 0.16 +0.4 418,131 + Pf6N2E2_352 0.20 +0.8 418,131 + Pf6N2E2_352 0.20 -2.2 418,132 - Pf6N2E2_352 0.20 +0.2 418,132 - Pf6N2E2_352 0.20 -0.8 418,132 - Pf6N2E2_352 0.20 -1.7 418,317 + Pf6N2E2_352 0.36 +0.5 418,318 - Pf6N2E2_352 0.36 +0.5 418,318 - Pf6N2E2_352 0.36 +0.0 418,335 - Pf6N2E2_352 0.38 +1.0 418,496 + Pf6N2E2_352 0.51 +1.0 418,497 - Pf6N2E2_352 0.51 +0.2 418,653 + Pf6N2E2_352 0.65 -2.0 418,654 - Pf6N2E2_352 0.65 +1.3
Or see this region's nucleotide sequence