Experiment: RCH2 defined with Thallium(I) acetate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1956 and Pf6N2E2_1957 are separated by 3 nucleotides Pf6N2E2_1957 and Pf6N2E2_1958 are separated by 170 nucleotides Pf6N2E2_1958 and Pf6N2E2_1959 are separated by 188 nucleotides
Pf6N2E2_1956 - Outer membrane protein romA, at 2,320,609 to 2,321,745
_1956
Pf6N2E2_1957 - putative inner membrane protein, at 2,321,749 to 2,323,065
_1957
Pf6N2E2_1958 - Predicted dinucleotide-binding enzymes, at 2,323,236 to 2,323,778
_1958
Pf6N2E2_1959 - D-sorbitol dehydrogenase (EC 1.1.1.14) (from data) , at 2,323,967 to 2,324,740
_1959
Position (kb)
2321
2322
2323
2324 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2320.891 kb on - strand, within Pf6N2E2_1956 at 2320.899 kb on + strand, within Pf6N2E2_1956 at 2320.899 kb on + strand, within Pf6N2E2_1956 at 2320.900 kb on - strand, within Pf6N2E2_1956 at 2320.900 kb on - strand, within Pf6N2E2_1956 at 2320.901 kb on + strand, within Pf6N2E2_1956 at 2320.902 kb on - strand, within Pf6N2E2_1956 at 2320.928 kb on - strand, within Pf6N2E2_1956 at 2320.981 kb on + strand, within Pf6N2E2_1956 at 2320.981 kb on + strand, within Pf6N2E2_1956 at 2320.982 kb on - strand, within Pf6N2E2_1956 at 2321.049 kb on - strand, within Pf6N2E2_1956 at 2321.087 kb on - strand, within Pf6N2E2_1956 at 2321.102 kb on - strand, within Pf6N2E2_1956 at 2321.159 kb on + strand, within Pf6N2E2_1956 at 2321.160 kb on - strand, within Pf6N2E2_1956 at 2321.160 kb on - strand, within Pf6N2E2_1956 at 2321.276 kb on - strand, within Pf6N2E2_1956 at 2321.276 kb on - strand, within Pf6N2E2_1956 at 2321.419 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956 at 2321.651 kb on - strand at 2321.743 kb on + strand at 2321.743 kb on + strand at 2321.744 kb on - strand at 2321.830 kb on + strand at 2321.830 kb on + strand at 2321.831 kb on - strand at 2321.858 kb on - strand at 2321.858 kb on - strand at 2321.932 kb on - strand, within Pf6N2E2_1957 at 2322.169 kb on - strand, within Pf6N2E2_1957 at 2322.206 kb on + strand, within Pf6N2E2_1957 at 2322.344 kb on + strand, within Pf6N2E2_1957 at 2322.451 kb on + strand, within Pf6N2E2_1957 at 2322.484 kb on + strand, within Pf6N2E2_1957 at 2322.508 kb on + strand, within Pf6N2E2_1957 at 2322.508 kb on + strand, within Pf6N2E2_1957 at 2322.611 kb on + strand, within Pf6N2E2_1957 at 2322.807 kb on + strand, within Pf6N2E2_1957 at 2322.966 kb on + strand at 2322.966 kb on + strand at 2322.967 kb on - strand at 2322.967 kb on - strand at 2322.967 kb on - strand at 2323.029 kb on + strand at 2323.071 kb on - strand at 2323.201 kb on + strand at 2323.201 kb on + strand at 2323.202 kb on - strand at 2323.202 kb on - strand at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.462 kb on - strand, within Pf6N2E2_1958 at 2323.507 kb on - strand, within Pf6N2E2_1958 at 2323.777 kb on - strand at 2323.777 kb on - strand at 2323.800 kb on + strand at 2323.804 kb on + strand at 2323.805 kb on - strand at 2323.805 kb on - strand at 2323.805 kb on - strand at 2323.824 kb on + strand at 2323.999 kb on - strand at 2324.016 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Thallium(I) acetate 0.002 mg/ml remove 2,320,891 - Pf6N2E2_1956 0.25 +0.4 2,320,899 + Pf6N2E2_1956 0.26 -0.4 2,320,899 + Pf6N2E2_1956 0.26 +0.8 2,320,900 - Pf6N2E2_1956 0.26 +0.2 2,320,900 - Pf6N2E2_1956 0.26 -0.5 2,320,901 + Pf6N2E2_1956 0.26 -1.5 2,320,902 - Pf6N2E2_1956 0.26 -0.4 2,320,928 - Pf6N2E2_1956 0.28 +1.6 2,320,981 + Pf6N2E2_1956 0.33 +1.4 2,320,981 + Pf6N2E2_1956 0.33 +1.6 2,320,982 - Pf6N2E2_1956 0.33 +1.8 2,321,049 - Pf6N2E2_1956 0.39 -0.6 2,321,087 - Pf6N2E2_1956 0.42 +0.1 2,321,102 - Pf6N2E2_1956 0.43 +0.2 2,321,159 + Pf6N2E2_1956 0.48 -0.9 2,321,160 - Pf6N2E2_1956 0.48 -0.1 2,321,160 - Pf6N2E2_1956 0.48 +0.5 2,321,276 - Pf6N2E2_1956 0.59 +0.6 2,321,276 - Pf6N2E2_1956 0.59 +1.6 2,321,419 + Pf6N2E2_1956 0.71 -1.0 2,321,443 + Pf6N2E2_1956 0.73 -0.4 2,321,443 + Pf6N2E2_1956 0.73 -0.4 2,321,443 + Pf6N2E2_1956 0.73 +0.2 2,321,651 - +0.9 2,321,743 + -0.4 2,321,743 + -0.3 2,321,744 - -0.8 2,321,830 + -0.6 2,321,830 + -2.4 2,321,831 - -1.0 2,321,858 - +1.6 2,321,858 - -1.5 2,321,932 - Pf6N2E2_1957 0.14 +0.5 2,322,169 - Pf6N2E2_1957 0.32 -0.2 2,322,206 + Pf6N2E2_1957 0.35 -1.4 2,322,344 + Pf6N2E2_1957 0.45 -1.4 2,322,451 + Pf6N2E2_1957 0.53 -0.4 2,322,484 + Pf6N2E2_1957 0.56 -0.6 2,322,508 + Pf6N2E2_1957 0.58 +0.6 2,322,508 + Pf6N2E2_1957 0.58 -0.4 2,322,611 + Pf6N2E2_1957 0.65 -2.3 2,322,807 + Pf6N2E2_1957 0.80 -1.2 2,322,966 + +0.6 2,322,966 + -0.0 2,322,967 - +0.2 2,322,967 - -1.0 2,322,967 - +0.4 2,323,029 + -1.7 2,323,071 - +0.6 2,323,201 + +1.4 2,323,201 + -0.3 2,323,202 - +0.8 2,323,202 - -1.1 2,323,459 + Pf6N2E2_1958 0.41 +0.4 2,323,459 + Pf6N2E2_1958 0.41 -1.2 2,323,459 + Pf6N2E2_1958 0.41 +0.9 2,323,459 + Pf6N2E2_1958 0.41 -0.6 2,323,462 - Pf6N2E2_1958 0.42 +0.3 2,323,507 - Pf6N2E2_1958 0.50 +0.4 2,323,777 - +1.5 2,323,777 - +0.6 2,323,800 + -1.4 2,323,804 + +0.0 2,323,805 - +0.7 2,323,805 - +3.2 2,323,805 - -1.0 2,323,824 + +1.6 2,323,999 - +0.2 2,324,016 + -1.0
Or see this region's nucleotide sequence