Experiment: RCH2 defined with Thallium(I) acetate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1382 and Pf6N2E2_1383 are separated by 408 nucleotides Pf6N2E2_1383 and Pf6N2E2_1384 are separated by 386 nucleotides
Pf6N2E2_1382 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 1,622,393 to 1,624,168
_1382
Pf6N2E2_1383 - YD repeat protein, at 1,624,577 to 1,625,677
_1383
Pf6N2E2_1384 - SoxH protein, homolog, at 1,626,064 to 1,626,993
_1384
Position (kb)
1624
1625
1626 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1623.811 kb on - strand, within Pf6N2E2_1382 at 1623.861 kb on + strand, within Pf6N2E2_1382 at 1623.861 kb on + strand, within Pf6N2E2_1382 at 1623.862 kb on - strand, within Pf6N2E2_1382 at 1623.877 kb on - strand, within Pf6N2E2_1382 at 1623.877 kb on - strand, within Pf6N2E2_1382 at 1623.877 kb on - strand, within Pf6N2E2_1382 at 1623.877 kb on - strand, within Pf6N2E2_1382 at 1624.002 kb on + strand at 1624.003 kb on - strand at 1624.195 kb on + strand at 1624.288 kb on - strand at 1624.288 kb on - strand at 1624.288 kb on - strand at 1624.288 kb on - strand at 1624.359 kb on - strand at 1624.441 kb on - strand at 1624.441 kb on - strand at 1624.467 kb on - strand at 1624.472 kb on - strand at 1624.505 kb on + strand at 1624.506 kb on - strand at 1624.506 kb on - strand at 1624.660 kb on - strand at 1624.673 kb on - strand at 1624.692 kb on - strand, within Pf6N2E2_1383 at 1624.696 kb on - strand, within Pf6N2E2_1383 at 1624.796 kb on - strand, within Pf6N2E2_1383 at 1624.796 kb on - strand, within Pf6N2E2_1383 at 1624.833 kb on - strand, within Pf6N2E2_1383 at 1624.900 kb on - strand, within Pf6N2E2_1383 at 1625.015 kb on - strand, within Pf6N2E2_1383 at 1625.170 kb on + strand, within Pf6N2E2_1383 at 1625.194 kb on - strand, within Pf6N2E2_1383 at 1625.298 kb on + strand, within Pf6N2E2_1383 at 1625.477 kb on - strand, within Pf6N2E2_1383 at 1625.501 kb on + strand, within Pf6N2E2_1383 at 1625.515 kb on - strand, within Pf6N2E2_1383 at 1625.608 kb on + strand at 1625.608 kb on + strand at 1625.609 kb on - strand at 1625.677 kb on + strand at 1625.677 kb on + strand at 1625.678 kb on - strand at 1625.689 kb on + strand at 1625.689 kb on + strand at 1625.690 kb on - strand at 1625.690 kb on - strand at 1625.690 kb on - strand at 1625.693 kb on - strand at 1625.693 kb on - strand at 1625.745 kb on - strand at 1625.899 kb on + strand at 1625.997 kb on + strand at 1625.998 kb on - strand at 1625.998 kb on - strand at 1626.141 kb on - strand at 1626.254 kb on + strand, within Pf6N2E2_1384 at 1626.404 kb on + strand, within Pf6N2E2_1384 at 1626.404 kb on + strand, within Pf6N2E2_1384 at 1626.404 kb on + strand, within Pf6N2E2_1384 at 1626.405 kb on - strand, within Pf6N2E2_1384 at 1626.405 kb on - strand, within Pf6N2E2_1384 at 1626.590 kb on + strand, within Pf6N2E2_1384 at 1626.591 kb on - strand, within Pf6N2E2_1384 at 1626.591 kb on - strand, within Pf6N2E2_1384
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Thallium(I) acetate 0.002 mg/ml remove 1,623,811 - Pf6N2E2_1382 0.80 -1.0 1,623,861 + Pf6N2E2_1382 0.83 +0.0 1,623,861 + Pf6N2E2_1382 0.83 -0.7 1,623,862 - Pf6N2E2_1382 0.83 +1.1 1,623,877 - Pf6N2E2_1382 0.84 +0.8 1,623,877 - Pf6N2E2_1382 0.84 -0.7 1,623,877 - Pf6N2E2_1382 0.84 +2.3 1,623,877 - Pf6N2E2_1382 0.84 -0.7 1,624,002 + -3.2 1,624,003 - +1.5 1,624,195 + +0.3 1,624,288 - +0.1 1,624,288 - -0.0 1,624,288 - -0.7 1,624,288 - -1.6 1,624,359 - +4.6 1,624,441 - +0.3 1,624,441 - +1.3 1,624,467 - +0.1 1,624,472 - +2.7 1,624,505 + +0.5 1,624,506 - +1.3 1,624,506 - -0.7 1,624,660 - -1.0 1,624,673 - -0.8 1,624,692 - Pf6N2E2_1383 0.10 -1.5 1,624,696 - Pf6N2E2_1383 0.11 +0.3 1,624,796 - Pf6N2E2_1383 0.20 -0.9 1,624,796 - Pf6N2E2_1383 0.20 -2.0 1,624,833 - Pf6N2E2_1383 0.23 +1.5 1,624,900 - Pf6N2E2_1383 0.29 -2.0 1,625,015 - Pf6N2E2_1383 0.40 +0.3 1,625,170 + Pf6N2E2_1383 0.54 +0.3 1,625,194 - Pf6N2E2_1383 0.56 -0.7 1,625,298 + Pf6N2E2_1383 0.65 -0.7 1,625,477 - Pf6N2E2_1383 0.82 -0.7 1,625,501 + Pf6N2E2_1383 0.84 -1.7 1,625,515 - Pf6N2E2_1383 0.85 -0.5 1,625,608 + -0.1 1,625,608 + -1.7 1,625,609 - -1.8 1,625,677 + -0.1 1,625,677 + -2.2 1,625,678 - -1.7 1,625,689 + +0.6 1,625,689 + -1.0 1,625,690 - -0.0 1,625,690 - +0.9 1,625,690 - -0.7 1,625,693 - +0.3 1,625,693 - +0.0 1,625,745 - +1.0 1,625,899 + -0.7 1,625,997 + +1.0 1,625,998 - -1.2 1,625,998 - +1.7 1,626,141 - -1.2 1,626,254 + Pf6N2E2_1384 0.20 +0.8 1,626,404 + Pf6N2E2_1384 0.37 +2.3 1,626,404 + Pf6N2E2_1384 0.37 +1.2 1,626,404 + Pf6N2E2_1384 0.37 +0.3 1,626,405 - Pf6N2E2_1384 0.37 -0.7 1,626,405 - Pf6N2E2_1384 0.37 +1.0 1,626,590 + Pf6N2E2_1384 0.57 +0.7 1,626,591 - Pf6N2E2_1384 0.57 +0.3 1,626,591 - Pf6N2E2_1384 0.57 -2.2
Or see this region's nucleotide sequence