Experiment: RCH2 defined with Thallium(I) acetate 0.002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_999 and Pf6N2E2_1000 are separated by 77 nucleotides Pf6N2E2_1000 and Pf6N2E2_1001 are separated by 765 nucleotides
Pf6N2E2_999 - Alcohol dehydrogenase (EC 1.1.1.1), at 1,168,005 to 1,169,069
_999
Pf6N2E2_1000 - hypothetical protein, at 1,169,147 to 1,169,530
_1000
Pf6N2E2_1001 - 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27), at 1,170,296 to 1,171,150
_1001
Position (kb)
1169
1170 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1168.270 kb on + strand, within Pf6N2E2_999 at 1168.270 kb on + strand, within Pf6N2E2_999 at 1168.270 kb on + strand, within Pf6N2E2_999 at 1168.270 kb on + strand, within Pf6N2E2_999 at 1168.270 kb on + strand, within Pf6N2E2_999 at 1168.271 kb on - strand, within Pf6N2E2_999 at 1168.271 kb on - strand, within Pf6N2E2_999 at 1168.271 kb on - strand, within Pf6N2E2_999 at 1168.271 kb on - strand, within Pf6N2E2_999 at 1168.271 kb on - strand, within Pf6N2E2_999 at 1168.275 kb on + strand, within Pf6N2E2_999 at 1168.296 kb on - strand, within Pf6N2E2_999 at 1168.371 kb on + strand, within Pf6N2E2_999 at 1168.371 kb on + strand, within Pf6N2E2_999 at 1168.371 kb on + strand, within Pf6N2E2_999 at 1168.371 kb on + strand, within Pf6N2E2_999 at 1168.372 kb on - strand, within Pf6N2E2_999 at 1168.408 kb on + strand, within Pf6N2E2_999 at 1168.408 kb on + strand, within Pf6N2E2_999 at 1168.408 kb on + strand, within Pf6N2E2_999 at 1168.531 kb on + strand, within Pf6N2E2_999 at 1168.531 kb on + strand, within Pf6N2E2_999 at 1168.618 kb on + strand, within Pf6N2E2_999 at 1168.619 kb on - strand, within Pf6N2E2_999 at 1168.619 kb on - strand, within Pf6N2E2_999 at 1168.619 kb on - strand, within Pf6N2E2_999 at 1168.619 kb on - strand, within Pf6N2E2_999 at 1168.714 kb on + strand, within Pf6N2E2_999 at 1168.714 kb on + strand, within Pf6N2E2_999 at 1168.714 kb on + strand, within Pf6N2E2_999 at 1168.714 kb on + strand, within Pf6N2E2_999 at 1168.715 kb on - strand, within Pf6N2E2_999 at 1168.715 kb on - strand, within Pf6N2E2_999 at 1168.715 kb on - strand, within Pf6N2E2_999 at 1168.715 kb on - strand, within Pf6N2E2_999 at 1169.053 kb on + strand at 1169.054 kb on - strand at 1169.058 kb on - strand at 1169.058 kb on - strand at 1169.122 kb on - strand at 1169.122 kb on - strand at 1169.148 kb on + strand at 1169.148 kb on + strand at 1169.149 kb on - strand at 1169.340 kb on + strand, within Pf6N2E2_1000 at 1169.340 kb on + strand, within Pf6N2E2_1000 at 1169.341 kb on - strand, within Pf6N2E2_1000 at 1169.341 kb on - strand, within Pf6N2E2_1000 at 1169.341 kb on - strand, within Pf6N2E2_1000 at 1169.341 kb on - strand, within Pf6N2E2_1000 at 1169.346 kb on + strand, within Pf6N2E2_1000 at 1169.346 kb on + strand, within Pf6N2E2_1000 at 1169.346 kb on + strand, within Pf6N2E2_1000 at 1169.347 kb on - strand, within Pf6N2E2_1000 at 1169.347 kb on - strand, within Pf6N2E2_1000 at 1169.347 kb on - strand, within Pf6N2E2_1000 at 1169.347 kb on - strand, within Pf6N2E2_1000 at 1169.373 kb on + strand, within Pf6N2E2_1000 at 1169.373 kb on + strand, within Pf6N2E2_1000 at 1169.374 kb on - strand, within Pf6N2E2_1000 at 1169.374 kb on - strand, within Pf6N2E2_1000 at 1169.374 kb on - strand, within Pf6N2E2_1000 at 1169.434 kb on - strand, within Pf6N2E2_1000 at 1169.451 kb on + strand, within Pf6N2E2_1000 at 1169.451 kb on + strand, within Pf6N2E2_1000 at 1169.476 kb on - strand, within Pf6N2E2_1000 at 1169.534 kb on - strand at 1169.562 kb on + strand at 1169.563 kb on - strand at 1169.563 kb on - strand at 1169.563 kb on - strand at 1169.563 kb on - strand at 1169.563 kb on - strand at 1169.563 kb on - strand at 1169.563 kb on - strand at 1169.563 kb on - strand at 1169.563 kb on - strand at 1169.563 kb on - strand at 1169.563 kb on - strand at 1169.563 kb on - strand at 1169.563 kb on - strand at 1169.611 kb on - strand at 1169.611 kb on - strand at 1169.645 kb on - strand at 1169.683 kb on - strand at 1169.738 kb on + strand at 1169.808 kb on + strand at 1169.808 kb on + strand at 1169.808 kb on + strand at 1169.808 kb on + strand at 1169.808 kb on + strand at 1169.808 kb on + strand at 1169.808 kb on + strand at 1169.808 kb on + strand at 1169.809 kb on - strand at 1169.809 kb on - strand at 1169.809 kb on - strand at 1169.809 kb on - strand at 1169.857 kb on - strand at 1169.882 kb on - strand at 1169.938 kb on + strand at 1169.938 kb on + strand at 1169.938 kb on + strand at 1169.938 kb on + strand at 1169.938 kb on + strand at 1169.938 kb on + strand at 1169.938 kb on + strand at 1169.939 kb on - strand at 1169.939 kb on - strand at 1169.939 kb on - strand at 1169.939 kb on - strand at 1169.939 kb on - strand at 1169.939 kb on - strand at 1169.939 kb on - strand at 1169.939 kb on - strand at 1169.939 kb on - strand at 1169.939 kb on - strand at 1169.939 kb on - strand at 1169.939 kb on - strand at 1169.942 kb on + strand at 1169.942 kb on + strand at 1169.942 kb on + strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.943 kb on - strand at 1169.946 kb on + strand at 1169.947 kb on - strand at 1169.947 kb on - strand at 1169.947 kb on - strand at 1169.947 kb on - strand at 1169.947 kb on - strand at 1169.947 kb on - strand at 1169.947 kb on - strand at 1170.015 kb on - strand at 1170.045 kb on - strand at 1170.070 kb on + strand at 1170.077 kb on + strand at 1170.098 kb on + strand at 1170.098 kb on + strand at 1170.098 kb on + strand at 1170.099 kb on - strand at 1170.099 kb on - strand at 1170.205 kb on - strand at 1170.224 kb on + strand at 1170.225 kb on - strand at 1170.227 kb on + strand at 1170.228 kb on - strand at 1170.228 kb on - strand at 1170.228 kb on - strand at 1170.245 kb on + strand at 1170.245 kb on + strand at 1170.246 kb on - strand at 1170.291 kb on + strand at 1170.292 kb on - strand at 1170.330 kb on - strand at 1170.330 kb on - strand at 1170.353 kb on + strand at 1170.402 kb on + strand, within Pf6N2E2_1001 at 1170.403 kb on - strand, within Pf6N2E2_1001 at 1170.425 kb on + strand, within Pf6N2E2_1001
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with Thallium(I) acetate 0.002 mg/ml remove 1,168,270 + Pf6N2E2_999 0.25 -0.2 1,168,270 + Pf6N2E2_999 0.25 +1.2 1,168,270 + Pf6N2E2_999 0.25 +0.6 1,168,270 + Pf6N2E2_999 0.25 +1.4 1,168,270 + Pf6N2E2_999 0.25 +0.4 1,168,271 - Pf6N2E2_999 0.25 +0.4 1,168,271 - Pf6N2E2_999 0.25 -0.9 1,168,271 - Pf6N2E2_999 0.25 -0.8 1,168,271 - Pf6N2E2_999 0.25 -1.4 1,168,271 - Pf6N2E2_999 0.25 +1.1 1,168,275 + Pf6N2E2_999 0.25 -0.2 1,168,296 - Pf6N2E2_999 0.27 -0.4 1,168,371 + Pf6N2E2_999 0.34 -0.0 1,168,371 + Pf6N2E2_999 0.34 -1.2 1,168,371 + Pf6N2E2_999 0.34 -1.2 1,168,371 + Pf6N2E2_999 0.34 +0.7 1,168,372 - Pf6N2E2_999 0.34 -1.1 1,168,408 + Pf6N2E2_999 0.38 -2.2 1,168,408 + Pf6N2E2_999 0.38 -0.4 1,168,408 + Pf6N2E2_999 0.38 +1.0 1,168,531 + Pf6N2E2_999 0.49 +0.9 1,168,531 + Pf6N2E2_999 0.49 -0.1 1,168,618 + Pf6N2E2_999 0.58 -0.6 1,168,619 - Pf6N2E2_999 0.58 +0.4 1,168,619 - Pf6N2E2_999 0.58 -2.5 1,168,619 - Pf6N2E2_999 0.58 +0.4 1,168,619 - Pf6N2E2_999 0.58 +0.4 1,168,714 + Pf6N2E2_999 0.67 +0.4 1,168,714 + Pf6N2E2_999 0.67 -0.8 1,168,714 + Pf6N2E2_999 0.67 +1.7 1,168,714 + Pf6N2E2_999 0.67 +0.4 1,168,715 - Pf6N2E2_999 0.67 +0.6 1,168,715 - Pf6N2E2_999 0.67 -0.5 1,168,715 - Pf6N2E2_999 0.67 +0.1 1,168,715 - Pf6N2E2_999 0.67 +1.8 1,169,053 + -0.2 1,169,054 - +1.9 1,169,058 - -0.3 1,169,058 - +0.9 1,169,122 - -0.6 1,169,122 - +0.4 1,169,148 + +1.7 1,169,148 + +0.1 1,169,149 - +1.1 1,169,340 + Pf6N2E2_1000 0.50 -0.2 1,169,340 + Pf6N2E2_1000 0.50 -0.2 1,169,341 - Pf6N2E2_1000 0.51 -1.6 1,169,341 - Pf6N2E2_1000 0.51 +1.4 1,169,341 - Pf6N2E2_1000 0.51 -0.2 1,169,341 - Pf6N2E2_1000 0.51 -1.4 1,169,346 + Pf6N2E2_1000 0.52 -0.6 1,169,346 + Pf6N2E2_1000 0.52 +0.4 1,169,346 + Pf6N2E2_1000 0.52 +0.4 1,169,347 - Pf6N2E2_1000 0.52 +0.4 1,169,347 - Pf6N2E2_1000 0.52 -0.3 1,169,347 - Pf6N2E2_1000 0.52 -0.6 1,169,347 - Pf6N2E2_1000 0.52 -1.5 1,169,373 + Pf6N2E2_1000 0.59 +0.1 1,169,373 + Pf6N2E2_1000 0.59 +0.6 1,169,374 - Pf6N2E2_1000 0.59 +0.4 1,169,374 - Pf6N2E2_1000 0.59 -1.2 1,169,374 - Pf6N2E2_1000 0.59 -1.4 1,169,434 - Pf6N2E2_1000 0.75 +0.4 1,169,451 + Pf6N2E2_1000 0.79 -1.2 1,169,451 + Pf6N2E2_1000 0.79 -2.0 1,169,476 - Pf6N2E2_1000 0.86 -2.2 1,169,534 - +0.2 1,169,562 + -1.2 1,169,563 - +0.3 1,169,563 - +0.4 1,169,563 - -1.2 1,169,563 - -0.6 1,169,563 - +1.0 1,169,563 - -1.2 1,169,563 - -0.4 1,169,563 - -1.2 1,169,563 - -2.4 1,169,563 - -1.2 1,169,563 - -0.3 1,169,563 - -1.6 1,169,563 - -1.6 1,169,611 - +0.0 1,169,611 - -1.2 1,169,645 - -1.2 1,169,683 - -1.8 1,169,738 + +0.9 1,169,808 + -2.0 1,169,808 + -0.6 1,169,808 + -0.6 1,169,808 + -1.6 1,169,808 + +1.2 1,169,808 + +0.8 1,169,808 + -0.1 1,169,808 + -0.1 1,169,809 - +0.5 1,169,809 - -0.6 1,169,809 - -0.6 1,169,809 - -0.4 1,169,857 - +0.4 1,169,882 - -0.6 1,169,938 + +0.6 1,169,938 + -0.6 1,169,938 + -0.2 1,169,938 + -0.4 1,169,938 + +0.4 1,169,938 + +0.4 1,169,938 + -0.6 1,169,939 - +0.1 1,169,939 - +1.7 1,169,939 - +0.3 1,169,939 - +0.4 1,169,939 - +0.7 1,169,939 - -0.3 1,169,939 - -0.6 1,169,939 - -0.4 1,169,939 - +0.4 1,169,939 - +0.4 1,169,939 - -0.6 1,169,939 - -0.5 1,169,942 + +1.4 1,169,942 + -0.1 1,169,942 + +0.9 1,169,943 - -1.6 1,169,943 - -0.9 1,169,943 - +0.5 1,169,943 - +1.4 1,169,943 - -1.1 1,169,943 - +0.4 1,169,943 - -1.6 1,169,943 - -0.1 1,169,943 - +0.4 1,169,943 - -0.6 1,169,943 - +0.4 1,169,943 - -0.6 1,169,943 - +0.4 1,169,943 - +0.4 1,169,943 - -0.6 1,169,946 + +0.6 1,169,947 - -0.6 1,169,947 - +2.1 1,169,947 - -2.0 1,169,947 - -2.6 1,169,947 - -1.6 1,169,947 - +0.4 1,169,947 - +0.4 1,170,015 - +0.4 1,170,045 - -3.0 1,170,070 + +2.4 1,170,077 + -2.2 1,170,098 + +0.9 1,170,098 + +3.8 1,170,098 + +0.9 1,170,099 - -2.1 1,170,099 - -1.0 1,170,205 - +0.4 1,170,224 + -0.4 1,170,225 - -0.5 1,170,227 + -0.2 1,170,228 - -2.0 1,170,228 - +0.4 1,170,228 - -0.0 1,170,245 + +2.5 1,170,245 + +0.4 1,170,246 - +1.5 1,170,291 + -0.1 1,170,292 - +0.4 1,170,330 - -0.8 1,170,330 - +0.1 1,170,353 + -1.2 1,170,402 + Pf6N2E2_1001 0.12 -0.6 1,170,403 - Pf6N2E2_1001 0.13 -0.8 1,170,425 + Pf6N2E2_1001 0.15 +0.7
Or see this region's nucleotide sequence