Experiment: RCH2 defined with copper (II) chloride 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1988 and Pf6N2E2_1989 are separated by 118 nucleotides Pf6N2E2_1989 and Pf6N2E2_1990 are separated by 404 nucleotides Pf6N2E2_1990 and Pf6N2E2_1991 are separated by 257 nucleotides Pf6N2E2_1991 and Pf6N2E2_1992 are separated by 177 nucleotides Pf6N2E2_1992 and Pf6N2E2_1993 are separated by 160 nucleotides
Pf6N2E2_1988 - hypothetical protein, at 2,354,318 to 2,354,551
_1988
Pf6N2E2_1989 - hypothetical protein, at 2,354,670 to 2,354,927
_1989
Pf6N2E2_1990 - Possible exported protein, at 2,355,332 to 2,356,015
_1990
Pf6N2E2_1991 - hypothetical protein, at 2,356,273 to 2,356,407
_1991
Pf6N2E2_1992 - hypothetical protein, at 2,356,585 to 2,356,728
_1992
Pf6N2E2_1993 - putative exported protein, at 2,356,889 to 2,358,316
_1993
Position (kb)
2355
2356
2357 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2354.519 kb on + strand, within Pf6N2E2_1988 at 2354.724 kb on + strand, within Pf6N2E2_1989 at 2354.725 kb on - strand, within Pf6N2E2_1989 at 2354.725 kb on - strand, within Pf6N2E2_1989 at 2354.800 kb on - strand, within Pf6N2E2_1989 at 2355.140 kb on - strand at 2355.252 kb on + strand at 2355.294 kb on - strand at 2355.294 kb on - strand at 2355.294 kb on - strand at 2355.460 kb on - strand, within Pf6N2E2_1990 at 2355.503 kb on - strand, within Pf6N2E2_1990 at 2355.548 kb on + strand, within Pf6N2E2_1990 at 2355.549 kb on - strand, within Pf6N2E2_1990 at 2355.596 kb on + strand, within Pf6N2E2_1990 at 2355.596 kb on + strand, within Pf6N2E2_1990 at 2355.627 kb on - strand, within Pf6N2E2_1990 at 2355.665 kb on + strand, within Pf6N2E2_1990 at 2355.665 kb on + strand, within Pf6N2E2_1990 at 2355.684 kb on + strand, within Pf6N2E2_1990 at 2355.684 kb on + strand, within Pf6N2E2_1990 at 2355.685 kb on - strand, within Pf6N2E2_1990 at 2355.717 kb on - strand, within Pf6N2E2_1990 at 2355.717 kb on - strand, within Pf6N2E2_1990 at 2355.717 kb on - strand, within Pf6N2E2_1990 at 2355.769 kb on - strand, within Pf6N2E2_1990 at 2355.793 kb on + strand, within Pf6N2E2_1990 at 2355.873 kb on + strand, within Pf6N2E2_1990 at 2355.977 kb on - strand at 2356.088 kb on + strand at 2356.089 kb on - strand at 2356.162 kb on - strand at 2356.167 kb on - strand at 2356.167 kb on - strand at 2356.167 kb on - strand at 2356.167 kb on - strand at 2356.202 kb on + strand at 2356.232 kb on - strand at 2356.232 kb on - strand at 2356.326 kb on - strand, within Pf6N2E2_1991 at 2356.438 kb on - strand at 2356.439 kb on + strand at 2356.440 kb on - strand at 2356.440 kb on - strand at 2356.440 kb on - strand at 2356.464 kb on - strand at 2356.504 kb on - strand at 2356.511 kb on + strand at 2356.511 kb on + strand at 2356.512 kb on - strand at 2356.512 kb on - strand at 2356.512 kb on - strand at 2356.512 kb on - strand at 2356.568 kb on + strand at 2356.569 kb on - strand at 2356.579 kb on - strand at 2356.627 kb on + strand, within Pf6N2E2_1992
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with copper (II) chloride 0.03 mM remove 2,354,519 + Pf6N2E2_1988 0.86 +0.8 2,354,724 + Pf6N2E2_1989 0.21 +0.8 2,354,725 - Pf6N2E2_1989 0.21 +0.4 2,354,725 - Pf6N2E2_1989 0.21 +0.4 2,354,800 - Pf6N2E2_1989 0.50 +1.1 2,355,140 - +0.8 2,355,252 + -0.9 2,355,294 - -1.2 2,355,294 - +0.8 2,355,294 - +0.1 2,355,460 - Pf6N2E2_1990 0.19 -0.8 2,355,503 - Pf6N2E2_1990 0.25 -1.8 2,355,548 + Pf6N2E2_1990 0.32 -1.4 2,355,549 - Pf6N2E2_1990 0.32 -1.2 2,355,596 + Pf6N2E2_1990 0.39 +0.2 2,355,596 + Pf6N2E2_1990 0.39 -3.0 2,355,627 - Pf6N2E2_1990 0.43 +0.1 2,355,665 + Pf6N2E2_1990 0.49 -0.1 2,355,665 + Pf6N2E2_1990 0.49 -0.8 2,355,684 + Pf6N2E2_1990 0.51 +0.4 2,355,684 + Pf6N2E2_1990 0.51 -0.4 2,355,685 - Pf6N2E2_1990 0.52 -1.5 2,355,717 - Pf6N2E2_1990 0.56 +0.4 2,355,717 - Pf6N2E2_1990 0.56 -1.2 2,355,717 - Pf6N2E2_1990 0.56 -0.5 2,355,769 - Pf6N2E2_1990 0.64 -1.2 2,355,793 + Pf6N2E2_1990 0.67 +1.5 2,355,873 + Pf6N2E2_1990 0.79 -0.2 2,355,977 - +1.9 2,356,088 + -1.2 2,356,089 - -1.7 2,356,162 - -0.8 2,356,167 - -0.6 2,356,167 - +0.8 2,356,167 - +0.3 2,356,167 - +0.6 2,356,202 + +0.8 2,356,232 - -0.1 2,356,232 - -0.7 2,356,326 - Pf6N2E2_1991 0.39 -0.3 2,356,438 - +0.3 2,356,439 + -0.8 2,356,440 - +1.8 2,356,440 - -0.8 2,356,440 - -1.5 2,356,464 - -1.6 2,356,504 - +0.1 2,356,511 + -0.8 2,356,511 + -2.0 2,356,512 - +0.8 2,356,512 - +1.8 2,356,512 - +1.5 2,356,512 - -1.2 2,356,568 + +0.8 2,356,569 - +0.8 2,356,579 - -0.5 2,356,627 + Pf6N2E2_1992 0.29 -0.2
Or see this region's nucleotide sequence