Experiment: RCH2 defined with copper (II) chloride 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1318 and Pf6N2E2_1319 are separated by 182 nucleotides Pf6N2E2_1319 and Pf6N2E2_1320 are separated by 51 nucleotides Pf6N2E2_1320 and Pf6N2E2_1321 are separated by 78 nucleotides Pf6N2E2_1321 and Pf6N2E2_1322 overlap by 8 nucleotides
Pf6N2E2_1318 - FIG00553873: hypothetical protein, at 1,552,204 to 1,553,502
_1318
Pf6N2E2_1319 - FIG074102: hypothetical protein, at 1,553,685 to 1,554,050
_1319
Pf6N2E2_1320 - Putative response regulator, at 1,554,102 to 1,554,761
_1320
Pf6N2E2_1321 - MoaF protein precursor, at 1,554,840 to 1,555,661
_1321
Pf6N2E2_1322 - MoaE protein, at 1,555,654 to 1,556,454
_1322
Position (kb)
1554
1555 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.519 kb on + strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.520 kb on - strand at 1553.839 kb on - strand, within Pf6N2E2_1319 at 1553.874 kb on + strand, within Pf6N2E2_1319 at 1553.874 kb on + strand, within Pf6N2E2_1319 at 1553.874 kb on + strand, within Pf6N2E2_1319 at 1553.952 kb on + strand, within Pf6N2E2_1319 at 1554.033 kb on + strand at 1554.033 kb on + strand at 1554.153 kb on - strand at 1554.187 kb on - strand, within Pf6N2E2_1320 at 1554.187 kb on - strand, within Pf6N2E2_1320 at 1554.258 kb on + strand, within Pf6N2E2_1320 at 1554.258 kb on + strand, within Pf6N2E2_1320 at 1554.271 kb on - strand, within Pf6N2E2_1320 at 1554.379 kb on - strand, within Pf6N2E2_1320 at 1554.716 kb on - strand at 1554.779 kb on - strand at 1554.838 kb on + strand at 1554.839 kb on - strand at 1554.839 kb on - strand at 1555.073 kb on - strand, within Pf6N2E2_1321 at 1555.073 kb on - strand, within Pf6N2E2_1321 at 1555.075 kb on - strand, within Pf6N2E2_1321 at 1555.075 kb on - strand, within Pf6N2E2_1321 at 1555.366 kb on - strand, within Pf6N2E2_1321 at 1555.378 kb on - strand, within Pf6N2E2_1321 at 1555.459 kb on - strand, within Pf6N2E2_1321 at 1555.459 kb on - strand, within Pf6N2E2_1321 at 1555.480 kb on - strand, within Pf6N2E2_1321 at 1555.480 kb on - strand, within Pf6N2E2_1321 at 1555.630 kb on + strand at 1555.648 kb on - strand at 1555.669 kb on - strand at 1555.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with copper (II) chloride 0.03 mM remove 1,553,519 + +1.8 1,553,519 + +0.4 1,553,519 + -0.3 1,553,519 + +0.6 1,553,519 + -1.3 1,553,520 - -0.4 1,553,520 - -2.3 1,553,520 - +3.1 1,553,520 - -1.4 1,553,520 - -0.8 1,553,520 - +1.2 1,553,839 - Pf6N2E2_1319 0.42 +0.3 1,553,874 + Pf6N2E2_1319 0.52 +0.8 1,553,874 + Pf6N2E2_1319 0.52 -1.6 1,553,874 + Pf6N2E2_1319 0.52 -1.0 1,553,952 + Pf6N2E2_1319 0.73 +1.1 1,554,033 + +0.6 1,554,033 + +0.6 1,554,153 - -1.0 1,554,187 - Pf6N2E2_1320 0.13 +1.5 1,554,187 - Pf6N2E2_1320 0.13 +0.5 1,554,258 + Pf6N2E2_1320 0.24 +2.0 1,554,258 + Pf6N2E2_1320 0.24 -2.8 1,554,271 - Pf6N2E2_1320 0.26 +1.1 1,554,379 - Pf6N2E2_1320 0.42 +1.7 1,554,716 - -2.5 1,554,779 - -0.0 1,554,838 + -1.0 1,554,839 - -0.3 1,554,839 - -0.3 1,555,073 - Pf6N2E2_1321 0.28 +1.1 1,555,073 - Pf6N2E2_1321 0.28 +1.4 1,555,075 - Pf6N2E2_1321 0.29 -3.0 1,555,075 - Pf6N2E2_1321 0.29 -0.5 1,555,366 - Pf6N2E2_1321 0.64 +0.5 1,555,378 - Pf6N2E2_1321 0.65 -1.0 1,555,459 - Pf6N2E2_1321 0.75 -0.6 1,555,459 - Pf6N2E2_1321 0.75 -1.5 1,555,480 - Pf6N2E2_1321 0.78 -1.0 1,555,480 - Pf6N2E2_1321 0.78 -0.4 1,555,630 + -1.5 1,555,648 - -1.5 1,555,669 - +1.7 1,555,723 + -0.5
Or see this region's nucleotide sequence