Experiment: RCH2 defined with copper (II) chloride 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_810 and Pf6N2E2_811 are separated by 340 nucleotides Pf6N2E2_811 and Pf6N2E2_812 are separated by 70 nucleotides
Pf6N2E2_810 - Various polyols ABC transporter, periplasmic substrate-binding protein, at 942,259 to 943,569
_810
Pf6N2E2_811 - Transcriptional regulator of various polyols utilization, AraC family, at 943,910 to 944,815
_811
Pf6N2E2_812 - Glycosyltransferase, at 944,886 to 946,178
_812
Position (kb) 943
944
945 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 943.037 kb on + strand, within Pf6N2E2_810 at 943.038 kb on - strand, within Pf6N2E2_810 at 943.038 kb on - strand, within Pf6N2E2_810 at 943.038 kb on - strand, within Pf6N2E2_810 at 943.148 kb on + strand, within Pf6N2E2_810 at 943.149 kb on - strand, within Pf6N2E2_810 at 943.149 kb on - strand, within Pf6N2E2_810 at 943.149 kb on - strand, within Pf6N2E2_810 at 943.169 kb on + strand, within Pf6N2E2_810 at 943.170 kb on - strand, within Pf6N2E2_810 at 943.307 kb on + strand, within Pf6N2E2_810 at 943.308 kb on - strand, within Pf6N2E2_810 at 943.308 kb on - strand, within Pf6N2E2_810 at 943.612 kb on + strand at 943.612 kb on + strand at 943.613 kb on - strand at 943.613 kb on - strand at 943.613 kb on - strand at 943.658 kb on - strand at 943.841 kb on + strand at 943.841 kb on + strand at 943.843 kb on + strand at 943.844 kb on - strand at 944.053 kb on + strand, within Pf6N2E2_811 at 944.053 kb on + strand, within Pf6N2E2_811 at 944.053 kb on + strand, within Pf6N2E2_811 at 944.054 kb on - strand, within Pf6N2E2_811 at 944.154 kb on + strand, within Pf6N2E2_811 at 944.154 kb on + strand, within Pf6N2E2_811 at 944.154 kb on + strand, within Pf6N2E2_811 at 944.155 kb on - strand, within Pf6N2E2_811 at 944.212 kb on - strand, within Pf6N2E2_811 at 944.227 kb on - strand, within Pf6N2E2_811 at 944.304 kb on + strand, within Pf6N2E2_811 at 944.486 kb on + strand, within Pf6N2E2_811 at 944.581 kb on + strand, within Pf6N2E2_811 at 944.622 kb on + strand, within Pf6N2E2_811 at 944.623 kb on - strand, within Pf6N2E2_811 at 944.637 kb on + strand, within Pf6N2E2_811 at 944.638 kb on - strand, within Pf6N2E2_811 at 944.638 kb on - strand, within Pf6N2E2_811 at 944.688 kb on + strand, within Pf6N2E2_811 at 944.742 kb on + strand at 944.742 kb on + strand at 944.761 kb on + strand at 944.761 kb on + strand at 944.781 kb on + strand at 944.781 kb on + strand at 944.781 kb on + strand at 944.807 kb on - strand at 944.831 kb on - strand at 944.926 kb on + strand at 944.926 kb on + strand at 944.926 kb on + strand at 944.926 kb on + strand at 944.926 kb on + strand at 944.926 kb on + strand at 944.926 kb on + strand at 944.926 kb on + strand at 944.926 kb on + strand at 944.926 kb on + strand at 944.926 kb on + strand at 944.926 kb on + strand at 944.926 kb on + strand at 944.927 kb on - strand at 944.927 kb on - strand at 944.927 kb on - strand at 944.927 kb on - strand at 944.927 kb on - strand at 944.927 kb on - strand at 944.927 kb on - strand at 944.927 kb on - strand at 944.927 kb on - strand at 944.927 kb on - strand at 944.927 kb on - strand at 945.118 kb on + strand, within Pf6N2E2_812 at 945.118 kb on + strand, within Pf6N2E2_812 at 945.119 kb on - strand, within Pf6N2E2_812 at 945.119 kb on - strand, within Pf6N2E2_812 at 945.119 kb on - strand, within Pf6N2E2_812 at 945.369 kb on - strand, within Pf6N2E2_812 at 945.397 kb on + strand, within Pf6N2E2_812 at 945.398 kb on - strand, within Pf6N2E2_812 at 945.398 kb on - strand, within Pf6N2E2_812 at 945.398 kb on - strand, within Pf6N2E2_812 at 945.407 kb on - strand, within Pf6N2E2_812
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with copper (II) chloride 0.03 mM remove 943,037 + Pf6N2E2_810 0.59 -0.3 943,038 - Pf6N2E2_810 0.59 -0.3 943,038 - Pf6N2E2_810 0.59 +0.7 943,038 - Pf6N2E2_810 0.59 -0.3 943,148 + Pf6N2E2_810 0.68 +1.7 943,149 - Pf6N2E2_810 0.68 +0.7 943,149 - Pf6N2E2_810 0.68 -0.1 943,149 - Pf6N2E2_810 0.68 -0.7 943,169 + Pf6N2E2_810 0.69 +0.7 943,170 - Pf6N2E2_810 0.69 -0.6 943,307 + Pf6N2E2_810 0.80 +1.4 943,308 - Pf6N2E2_810 0.80 -0.6 943,308 - Pf6N2E2_810 0.80 +0.5 943,612 + +0.7 943,612 + +1.2 943,613 - -0.3 943,613 - +1.1 943,613 - -0.3 943,658 - -0.4 943,841 + +0.7 943,841 + -0.1 943,843 + -0.1 943,844 - +1.3 944,053 + Pf6N2E2_811 0.16 +1.6 944,053 + Pf6N2E2_811 0.16 -1.3 944,053 + Pf6N2E2_811 0.16 -0.3 944,054 - Pf6N2E2_811 0.16 -0.9 944,154 + Pf6N2E2_811 0.27 -2.3 944,154 + Pf6N2E2_811 0.27 -0.3 944,154 + Pf6N2E2_811 0.27 -1.3 944,155 - Pf6N2E2_811 0.27 -0.3 944,212 - Pf6N2E2_811 0.33 -0.6 944,227 - Pf6N2E2_811 0.35 +0.7 944,304 + Pf6N2E2_811 0.43 +0.3 944,486 + Pf6N2E2_811 0.64 +0.7 944,581 + Pf6N2E2_811 0.74 +0.4 944,622 + Pf6N2E2_811 0.79 -1.5 944,623 - Pf6N2E2_811 0.79 -0.9 944,637 + Pf6N2E2_811 0.80 +3.5 944,638 - Pf6N2E2_811 0.80 -1.6 944,638 - Pf6N2E2_811 0.80 -1.9 944,688 + Pf6N2E2_811 0.86 -1.6 944,742 + +0.5 944,742 + +1.5 944,761 + +0.5 944,761 + -2.1 944,781 + -0.3 944,781 + -0.2 944,781 + +0.2 944,807 - +0.8 944,831 - -1.0 944,926 + -0.9 944,926 + -0.2 944,926 + +0.9 944,926 + -0.3 944,926 + -1.1 944,926 + -0.9 944,926 + +1.3 944,926 + -1.6 944,926 + +0.1 944,926 + +0.7 944,926 + +0.8 944,926 + -2.8 944,926 + +0.7 944,927 - -0.8 944,927 - -0.1 944,927 - +0.5 944,927 - +0.3 944,927 - -0.0 944,927 - +1.1 944,927 - +1.4 944,927 - +0.5 944,927 - +0.3 944,927 - +1.7 944,927 - +1.1 945,118 + Pf6N2E2_812 0.18 -1.3 945,118 + Pf6N2E2_812 0.18 -1.3 945,119 - Pf6N2E2_812 0.18 +0.7 945,119 - Pf6N2E2_812 0.18 +0.7 945,119 - Pf6N2E2_812 0.18 -1.6 945,369 - Pf6N2E2_812 0.37 -1.1 945,397 + Pf6N2E2_812 0.40 -2.1 945,398 - Pf6N2E2_812 0.40 -0.1 945,398 - Pf6N2E2_812 0.40 +0.3 945,398 - Pf6N2E2_812 0.40 -0.3 945,407 - Pf6N2E2_812 0.40 -0.6
Or see this region's nucleotide sequence