Experiment: RCH2 defined with copper (II) chloride 0.03 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4978 and Pf6N2E2_4979 are separated by 274 nucleotides Pf6N2E2_4979 and Pf6N2E2_4980 are separated by 68 nucleotides Pf6N2E2_4980 and Pf6N2E2_4981 overlap by 27 nucleotides
Pf6N2E2_4978 - Ethanolamine permease, at 5,743,333 to 5,744,703
_4978
Pf6N2E2_4979 - Aldehyde dehydrogenase (EC 1.2.1.3), at 5,744,978 to 5,746,498
_4979
Pf6N2E2_4980 - hypothetical protein, at 5,746,567 to 5,747,094
_4980
Pf6N2E2_4981 - Nitrogen regulation protein NR(I), at 5,747,068 to 5,748,975
_4981
Position (kb) 5744
5745
5746
5747 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5743.993 kb on + strand, within Pf6N2E2_4978 at 5743.993 kb on + strand, within Pf6N2E2_4978 at 5743.994 kb on - strand, within Pf6N2E2_4978 at 5743.994 kb on - strand, within Pf6N2E2_4978 at 5744.015 kb on + strand, within Pf6N2E2_4978 at 5744.016 kb on - strand, within Pf6N2E2_4978 at 5744.181 kb on + strand, within Pf6N2E2_4978 at 5744.312 kb on + strand, within Pf6N2E2_4978 at 5744.313 kb on - strand, within Pf6N2E2_4978 at 5744.313 kb on - strand, within Pf6N2E2_4978 at 5744.367 kb on - strand, within Pf6N2E2_4978 at 5744.471 kb on + strand, within Pf6N2E2_4978 at 5744.472 kb on - strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.540 kb on + strand, within Pf6N2E2_4978 at 5744.541 kb on - strand, within Pf6N2E2_4978 at 5744.541 kb on - strand, within Pf6N2E2_4978 at 5744.541 kb on - strand, within Pf6N2E2_4978 at 5744.541 kb on - strand, within Pf6N2E2_4978 at 5744.541 kb on - strand, within Pf6N2E2_4978 at 5744.541 kb on - strand, within Pf6N2E2_4978 at 5744.603 kb on + strand at 5744.604 kb on - strand at 5744.694 kb on + strand at 5744.694 kb on + strand at 5744.695 kb on - strand at 5744.695 kb on - strand at 5744.703 kb on + strand at 5744.703 kb on + strand at 5744.703 kb on + strand at 5744.703 kb on + strand at 5744.704 kb on - strand at 5744.704 kb on - strand at 5744.704 kb on - strand at 5744.704 kb on - strand at 5744.704 kb on - strand at 5744.724 kb on - strand at 5744.724 kb on - strand at 5744.980 kb on - strand at 5744.980 kb on - strand at 5744.980 kb on - strand at 5744.980 kb on - strand at 5744.980 kb on - strand at 5745.006 kb on + strand at 5745.006 kb on + strand at 5745.036 kb on + strand at 5745.036 kb on + strand at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.348 kb on + strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.349 kb on - strand, within Pf6N2E2_4979 at 5745.351 kb on + strand, within Pf6N2E2_4979 at 5745.559 kb on - strand, within Pf6N2E2_4979 at 5745.559 kb on - strand, within Pf6N2E2_4979 at 5745.559 kb on - strand, within Pf6N2E2_4979 at 5746.053 kb on + strand, within Pf6N2E2_4979 at 5746.384 kb on + strand at 5746.384 kb on + strand at 5746.384 kb on + strand at 5746.384 kb on + strand at 5746.384 kb on + strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.385 kb on - strand at 5746.395 kb on + strand at 5746.395 kb on + strand at 5746.395 kb on + strand at 5746.395 kb on + strand at 5746.395 kb on + strand at 5746.396 kb on - strand at 5746.396 kb on - strand at 5746.396 kb on - strand at 5746.396 kb on - strand at 5746.434 kb on + strand at 5746.434 kb on + strand at 5746.434 kb on + strand at 5746.434 kb on + strand at 5746.434 kb on + strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.435 kb on - strand at 5746.438 kb on + strand at 5746.443 kb on + strand at 5746.443 kb on + strand at 5746.444 kb on - strand at 5746.444 kb on - strand at 5746.444 kb on - strand at 5746.444 kb on - strand at 5746.465 kb on + strand at 5746.525 kb on + strand at 5746.525 kb on + strand at 5746.525 kb on + strand at 5746.526 kb on - strand at 5746.526 kb on - strand at 5746.527 kb on + strand at 5746.527 kb on + strand at 5746.527 kb on + strand at 5746.528 kb on - strand at 5746.528 kb on - strand at 5746.528 kb on - strand at 5746.562 kb on + strand at 5746.562 kb on + strand at 5746.562 kb on + strand at 5746.563 kb on - strand at 5746.563 kb on - strand at 5746.563 kb on - strand at 5746.568 kb on + strand at 5746.784 kb on + strand, within Pf6N2E2_4980 at 5746.784 kb on + strand, within Pf6N2E2_4980 at 5746.784 kb on + strand, within Pf6N2E2_4980 at 5746.785 kb on - strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.786 kb on + strand, within Pf6N2E2_4980 at 5746.787 kb on - strand, within Pf6N2E2_4980 at 5746.956 kb on + strand, within Pf6N2E2_4980 at 5746.957 kb on - strand, within Pf6N2E2_4980 at 5746.957 kb on - strand, within Pf6N2E2_4980 at 5746.957 kb on - strand, within Pf6N2E2_4980 at 5747.059 kb on + strand at 5747.059 kb on + strand at 5747.062 kb on + strand at 5747.062 kb on + strand at 5747.063 kb on - strand at 5747.063 kb on - strand at 5747.063 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 defined with copper (II) chloride 0.03 mM remove 5,743,993 + Pf6N2E2_4978 0.48 -2.0 5,743,993 + Pf6N2E2_4978 0.48 +0.3 5,743,994 - Pf6N2E2_4978 0.48 -0.8 5,743,994 - Pf6N2E2_4978 0.48 -0.5 5,744,015 + Pf6N2E2_4978 0.50 +0.0 5,744,016 - Pf6N2E2_4978 0.50 +1.3 5,744,181 + Pf6N2E2_4978 0.62 -0.4 5,744,312 + Pf6N2E2_4978 0.71 +0.6 5,744,313 - Pf6N2E2_4978 0.71 -0.3 5,744,313 - Pf6N2E2_4978 0.71 -0.4 5,744,367 - Pf6N2E2_4978 0.75 -0.1 5,744,471 + Pf6N2E2_4978 0.83 -0.3 5,744,472 - Pf6N2E2_4978 0.83 +1.3 5,744,540 + Pf6N2E2_4978 0.88 -0.5 5,744,540 + Pf6N2E2_4978 0.88 +0.5 5,744,540 + Pf6N2E2_4978 0.88 -1.1 5,744,540 + Pf6N2E2_4978 0.88 +0.7 5,744,540 + Pf6N2E2_4978 0.88 +0.8 5,744,540 + Pf6N2E2_4978 0.88 +1.2 5,744,540 + Pf6N2E2_4978 0.88 -1.0 5,744,541 - Pf6N2E2_4978 0.88 -1.2 5,744,541 - Pf6N2E2_4978 0.88 -0.9 5,744,541 - Pf6N2E2_4978 0.88 +0.3 5,744,541 - Pf6N2E2_4978 0.88 +0.5 5,744,541 - Pf6N2E2_4978 0.88 +2.3 5,744,541 - Pf6N2E2_4978 0.88 +1.3 5,744,603 + -0.1 5,744,604 - +0.4 5,744,694 + +0.3 5,744,694 + +0.2 5,744,695 - +1.0 5,744,695 - +0.1 5,744,703 + -2.1 5,744,703 + +0.6 5,744,703 + +1.8 5,744,703 + -0.7 5,744,704 - +1.3 5,744,704 - +0.6 5,744,704 - +0.1 5,744,704 - -1.2 5,744,704 - +0.8 5,744,724 - -0.7 5,744,724 - -0.4 5,744,980 - +1.1 5,744,980 - +0.1 5,744,980 - -0.2 5,744,980 - -2.0 5,744,980 - -0.3 5,745,006 + +1.9 5,745,006 + +0.4 5,745,036 + -0.1 5,745,036 + +0.3 5,745,348 + Pf6N2E2_4979 0.24 -0.8 5,745,348 + Pf6N2E2_4979 0.24 +0.3 5,745,348 + Pf6N2E2_4979 0.24 -0.4 5,745,348 + Pf6N2E2_4979 0.24 -0.1 5,745,348 + Pf6N2E2_4979 0.24 -1.9 5,745,348 + Pf6N2E2_4979 0.24 -1.0 5,745,348 + Pf6N2E2_4979 0.24 -1.2 5,745,348 + Pf6N2E2_4979 0.24 +0.7 5,745,348 + Pf6N2E2_4979 0.24 -0.5 5,745,348 + Pf6N2E2_4979 0.24 +0.6 5,745,348 + Pf6N2E2_4979 0.24 -0.3 5,745,349 - Pf6N2E2_4979 0.24 +0.3 5,745,349 - Pf6N2E2_4979 0.24 +1.0 5,745,349 - Pf6N2E2_4979 0.24 +0.3 5,745,349 - Pf6N2E2_4979 0.24 -0.1 5,745,349 - Pf6N2E2_4979 0.24 +0.8 5,745,349 - Pf6N2E2_4979 0.24 +0.3 5,745,349 - Pf6N2E2_4979 0.24 +0.2 5,745,349 - Pf6N2E2_4979 0.24 -0.7 5,745,349 - Pf6N2E2_4979 0.24 -0.0 5,745,349 - Pf6N2E2_4979 0.24 +0.8 5,745,349 - Pf6N2E2_4979 0.24 +0.5 5,745,349 - Pf6N2E2_4979 0.24 -1.3 5,745,349 - Pf6N2E2_4979 0.24 -1.4 5,745,349 - Pf6N2E2_4979 0.24 -0.5 5,745,349 - Pf6N2E2_4979 0.24 -1.5 5,745,351 + Pf6N2E2_4979 0.25 -0.1 5,745,559 - Pf6N2E2_4979 0.38 -0.2 5,745,559 - Pf6N2E2_4979 0.38 +1.3 5,745,559 - Pf6N2E2_4979 0.38 -0.3 5,746,053 + Pf6N2E2_4979 0.71 -0.1 5,746,384 + +0.9 5,746,384 + -0.1 5,746,384 + +0.6 5,746,384 + -2.1 5,746,384 + +0.3 5,746,385 - +0.4 5,746,385 - -0.7 5,746,385 - +0.2 5,746,385 - -0.3 5,746,385 - -2.2 5,746,385 - +0.3 5,746,385 - -0.2 5,746,385 - +0.3 5,746,385 - -1.0 5,746,385 - -0.2 5,746,385 - -0.7 5,746,395 + +1.9 5,746,395 + -0.4 5,746,395 + -0.7 5,746,395 + +1.3 5,746,395 + +0.8 5,746,396 - -2.2 5,746,396 - -0.8 5,746,396 - +0.3 5,746,396 - -1.7 5,746,434 + +1.3 5,746,434 + +0.6 5,746,434 + -0.1 5,746,434 + -0.5 5,746,434 + +1.3 5,746,435 - +1.3 5,746,435 - +0.5 5,746,435 - +0.5 5,746,435 - -1.5 5,746,435 - -0.4 5,746,435 - +0.4 5,746,438 + -0.2 5,746,443 + -1.8 5,746,443 + +0.5 5,746,444 - +1.3 5,746,444 - -1.2 5,746,444 - -0.7 5,746,444 - +0.8 5,746,465 + -0.0 5,746,525 + -0.2 5,746,525 + +1.0 5,746,525 + +0.3 5,746,526 - -0.2 5,746,526 - +0.6 5,746,527 + +0.8 5,746,527 + +0.2 5,746,527 + -1.2 5,746,528 - -0.4 5,746,528 - +0.2 5,746,528 - -1.2 5,746,562 + -1.4 5,746,562 + +1.0 5,746,562 + -2.6 5,746,563 - +1.5 5,746,563 - -0.9 5,746,563 - +0.3 5,746,568 + -0.6 5,746,784 + Pf6N2E2_4980 0.41 -1.2 5,746,784 + Pf6N2E2_4980 0.41 +1.3 5,746,784 + Pf6N2E2_4980 0.41 +0.4 5,746,785 - Pf6N2E2_4980 0.41 +1.3 5,746,786 + Pf6N2E2_4980 0.41 -0.4 5,746,786 + Pf6N2E2_4980 0.41 +1.0 5,746,786 + Pf6N2E2_4980 0.41 -0.8 5,746,786 + Pf6N2E2_4980 0.41 -0.5 5,746,786 + Pf6N2E2_4980 0.41 +1.5 5,746,786 + Pf6N2E2_4980 0.41 +0.3 5,746,787 - Pf6N2E2_4980 0.42 +1.3 5,746,956 + Pf6N2E2_4980 0.74 -0.1 5,746,957 - Pf6N2E2_4980 0.74 +1.0 5,746,957 - Pf6N2E2_4980 0.74 -0.2 5,746,957 - Pf6N2E2_4980 0.74 +0.3 5,747,059 + +0.8 5,747,059 + -1.8 5,747,062 + -1.0 5,747,062 + +0.0 5,747,063 - -1.0 5,747,063 - +0.3 5,747,063 - +0.3
Or see this region's nucleotide sequence